Name: Onderzoek van anatomische kenmerken van retinale ganglioncellen onder door N-methyl-D-asparaginezuur (NMDA) geïnduceerde excitotoxiciteit (Video) | JoVE Journal
Uploaded: 2025-09-19T12:40:00.000+00:00
Duration: 7 min 11 s
Description: Ontdek hoe retinale ganglioncellen degenereren onder NMDA-geïnduceerde excitotoxiciteit met behulp van genetisch gerichte alkalische fosfatase-labeling in muizen. Deze methode onthult nieuwe fenotypen en inzichten in overlevingsmechanismen.

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onderzoek van anatomische kenmerken van retinale ganglioncellen onder door n-methyl-d-asparaginezuur (nmda) geïnduceerde excitotoxiciteit

Examination of Anatomical Features of Retinal Ganglion Cells Under N-methyl-D-aspartic Acid (NMDA)-induced Excitotoxicity

Onderzoek van anatomische kenmerken van retinale ganglioncellen onder door N-methyl-D-asparaginezuur (NMDA) geïnduceerde excitotoxiciteit

Retinal ganglion cells (RGCs) are the output neurons of the retina, responsible for transmitting visual information to the brain. In the mature mouse ...

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JoVE Journal

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Biologie

Intravitreale injectie is een veel gebruikte techniek in de visuele wetenschappen voor oculaire ziekten of als directe toepassing van lokale behandeling. Deze video toonde een protocol voor intravitreale injectie met een 1 ml injectiespuit met glazen pipet. Handige tips over het vermijden van grote bloeden en de lens beschadigd worden gegeven.

Intravitreale Injectie voor de oprichting van oogziekten Model

Deze video toont drie soorten morfometrische analyses van het netvlies, waaronder het meten van de binnenste nucleaire laag dikte, het kwantificeren van het aantal retinale ganglioncellen (RGC) en het meten van de afmetingen van RGC. De techniek kan bieden een eenvoudige, maar wetenschappelijk platform voor morfometrische analyses.

Morfometrische analyses van het netvlies secties

Deze video illustreert een methode, met behulp van een klinische 3 T scanner, voor contrast-versterkte MRI van de naïeve muis visuele projectie en voor herhaalde en longitudinale in vivo studies van de oogzenuw degeneratie geassocieerd met acute oogzenuw verpletteren letsel en chronische oogzenuw degeneratie in knock-out muizen (p50 KO).

In vivo beeldvorming van Optic Nerve Fiber Integrity by-Contrast Enhanced MRI in Muizen

Immunohistochemie protocollen worden gebruikt voor de lokalisatie van een specifiek eiwit in het netvlies te bestuderen. Calcium beeldvormende technieken worden gebruikt om calcium dynamiek in retinale ganglioncellen en hun axonen te bestuderen.

Immunohistochemische en Calcium Imaging Methods in Wholemount Rat Retina

Dit protocol beschrijft een eenvoudige methode om te isoleren en primaire muisknop cerebrale submodule neuronen (CGNs) van 6-7 dag oud pups, efficiënte transductie van CGNs voor verlies en winst van functie studies, kweken en modellering NMDA-geïnduceerde neuronale excitotoxicity, lage-kalium-veroorzaakte celdood, DNA-schade en oxidatieve stress met behulp van de zelfde cultuur-model.

Modelleren van neuronale dood en degeneratie in muis primaire cerebellaire submodule neuronen

Het doel van dit protocol is te vergemakkelijken van de studie van NMDA-receptoren (NMDAR) op een grotere schaal en het onderzoek van f effecten van kleine moleculen en hun therapeutische toepassingen.

Een High-throughput Calcium-flux Assay te studeren NMDA-receptoren met gevoeligheid voor Glycine/D-serine en glutamaat

In vivo beeldvorming is een krachtig hulpmiddel voor de studie van biologie in gezondheid en ziekte. Dit protocol beschrijft transpupillaire beeldvorming van het netvlies van de muis met een standaard microscoop met twee fotonen. Het demonstreert ook verschillende in vivoimaging-methoden om fluorescerend meerdere cellulaire cohorten van het netvlies te labelen.

Transpupillaire twee-fotonen in vivo beeldvorming van het netvlies van de muis

Dit artikel beschrijft een protocol om verschillen in basale redoxtoestand en redoxresponsen op acute verstoringen in primaire hippocampus- en corticale neuronen te bepalen met behulp van confocale levende microscopie. Het protocol kan met minimale aanpassingen worden toegepast op andere celtypen en microscopen.

Live imaging van de mitochondriale glutathion redoxtoestand in primaire neuronen met behulp van een ratiometrische indicator

Methanol kan worden gebruikt als een hulpmedium voor retinale preparaten voor de gehele montage van het netvlies en langdurige opslag, wat nuttig is voor het onderzoek van retinale ganglioncellen.

Op methanol gebaseerd hele-mountpreparaat voor het onderzoek van retinale ganglioncellen

Deze video beschrijft de methode van retrograde etikettering van RGC door het toepassen van fluoro-goud (FG) op het oppervlak van superieure colliculus (SC). Techniek omvat het boren van de schedel, opzuigen van de cortex, en het toepassen van gelatine spons over de gehele dorsale oppervlak van de SC.

Retrograde Labeling van retinale ganglioncellen door toepassing van fluor-Gold op het oppervlak van Superior colliculus

Ontdek hoe retinale ganglioncellen degenereren onder NMDA-geïnduceerde excitotoxiciteit met behulp van genetisch gerichte alkalische fosfatase-labeling in muizen. Deze methode onthult nieuwe fenotypen en inzichten in overlevingsmechanismen.

Onderzoek van anatomische kenmerken van retinale ganglioncellen onder door N-methyl-D-asparaginezuur (NMDA) geïnduceerde excitotoxiciteit (Video) | JoVE Journal

Onderzoek van anatomische kenmerken van retinale ganglioncellen onder door N-methyl-D-asparaginezuur (NMDA) geïnduceerde excitotoxiciteit

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