Name: Translationeel relevant tumorresectiemodel voor muispreklinische modellen van orale plaveiselcelcarcinoom (Video) | JoVE Journal
Uploaded: 2026-04-03T12:45:00.000+00:00
Duration: 7 min 59 s
Description: Leer een orthotopisch tumorresectiemodel voor oraal plaveiselcelcarcinoom bij muizen. Deze methode gebruikt Mach Two-cellen en subtotale resectie om tumorrecidief en resistentie te bestuderen.

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<li>Lee, A. M., Weaver, A. N., Acosta, P., Harris, L., Bowles, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Review+of+current+and+future+medical+treatments+in+head+and+neck+squamous+cell+carcinoma%5BTitle%5D)+AND+%22Cancers+%28Basel%29%22%5BJournal%5D)">Review of current and future medical treatments in head and neck squamous cell carcinoma.</a> Cancers (Basel). 16 (20), 3488(2024).</li>
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<li>Cossarizza, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Guidelines+for+the+use+of+flow+cytometry+and+cell+sorting+in+immunological+studies+%28third+edition%29%5BTitle%5D)+AND+%22Eur+J+Immunol%22%5BJournal%5D)">Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).</a> Eur J Immunol. 51 (12), 2708-3145 (2021).</li>
<li>Gurina, T. S., Simms, L. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aGurina%2C+TS+%22Histology%2C+staining%22">Histology, staining</a>. , StatPearls Publishing. (2025).</li>
<li>Cognetti, D. M., Weber, R. S., Lai, S. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Head+and+neck+cancer%3A+An+evolving+treatment+paradigm%5BTitle%5D)+AND+%22Cancer%22%5BJournal%5D)">Head and neck cancer: An evolving treatment paradigm.</a> Cancer. 113 (7 Suppl), 1911-1932 (2008).</li>
<li>Anderson, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+updated+review+on+head+and+neck+cancer+treatment+with+radiation+therapy%5BTitle%5D)+AND+%22Cancers+%28Basel%29%22%5BJournal%5D)">An updated review on head and neck cancer treatment with radiation therapy.</a> Cancers (Basel). 13 (19), 4912(2021).</li>
<li>Johnson, D. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Head+and+neck+squamous+cell+carcinoma%5BTitle%5D)+AND+%22Nat+Rev+Dis+Primers%22%5BJournal%5D)">Head and neck squamous cell carcinoma.</a> Nat Rev Dis Primers. 6 (1), 92(2020).</li>
<li>Friedman, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neoadjuvant+PD-1+immune+checkpoint+blockade+reverses+functional+immunodominance+among+tumor+antigen-specific+T+cells%5BTitle%5D)+AND+%22Clin+Cancer+Res%22%5BJournal%5D)">Neoadjuvant PD-1 immune checkpoint blockade reverses functional immunodominance among tumor antigen-specific T cells.</a> Clin Cancer Res. 26 (3), 679-689 (2020).</li>
<li>Zhou, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neoadjuvant+and+adjuvant+immunotherapy+in+the+treatment+of+oral+squamous+cell+carcinoma%5BTitle%5D)+AND+%22J+Biochem+Mol+Toxicol%22%5BJournal%5D)">Neoadjuvant and adjuvant immunotherapy in the treatment of oral squamous cell carcinoma.</a> J Biochem Mol Toxicol. 39 (3), e70199(2025).</li>
<li>Shibata, H., Saito, S., Uppaluri, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunotherapy+for+head+and+neck+cancer%3A+a+paradigm+shift+from+induction+chemotherapy+to+neoadjuvant+immunotherapy%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Immunotherapy for head and neck cancer: a paradigm shift from induction chemotherapy to neoadjuvant immunotherapy.</a> Front Oncol. 11, 727433(2021).</li>
<li>Saddawi-Konefka, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lymphatic-preserving+treatment+sequencing+with+immune+checkpoint+inhibition+unleashes+cDC1-dependent+antitumor+immunity+in+HNSCC%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">Lymphatic-preserving treatment sequencing with immune checkpoint inhibition unleashes cDC1-dependent antitumor immunity in HNSCC.</a> Nat Commun. 13 (1), 4298(2022).</li>
<li>Darragh, L. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Elective+nodal+irradiation+mitigates+local+and+systemic+immunity+generated+by+combination+radiation+and+immunotherapy+in+head+and+neck+tumors%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">Elective nodal irradiation mitigates local and systemic immunity generated by combination radiation and immunotherapy in head and neck tumors.</a> Nat Commun. 13 (1), 7015(2022).</li>
<li>Saddawi-Konefka, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Defining+the+role+of+immunotherapy+in+the+curative+treatment+of+locoregionally+advanced+head+and+neck+cancer%3A+Promises%2C+challenges%2C+and+opportunities%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Defining the role of immunotherapy in the curative treatment of locoregionally advanced head and neck cancer: Promises, challenges, and opportunities.</a> Front Oncol. 11, 738262(2021).</li>
</ol>

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translationeel relevant tumorresectiemodel voor muispreklinische modellen van orale plaveiselcelcarcinoom

Translationally-Relevant Tumor Resection Model for Murine Preclinical Models of Oral Squamous Cell Carcinoma

Translationeel relevant tumorresectiemodel voor muispreklinische modellen van orale plaveiselcelcarcinoom

Currently, the first-line treatment for the most common form of head and neck squamous cell carcinoma (HNSCC), oral cavity squamous cell carcinoma (OSCC), ...

translationally-relevant-tumor-resection-model-for-murine-preclinical

Research

JoVE Journal

Cancer Research

Kankeronderzoek

Twee technieken kunnen worden gebruikt om dit model te stellen: injectie van een kankercel suspensie in de blindedarmkeutels muur of transplantatie van een stukje van subcutane tumor op de blindedarm. Dit model is handig voor het bestuderen van de natuurlijke progressie van colorectale kanker en testen van nieuwe therapeutische middelen tegen colorectale kanker.

Orthotope Muis Model van colorectale kanker

Een uitgebreid overzicht van de technieken die betrokken zijn bij het genereren van een muismodel van orale kanker en kwantitatieve controle van de tumor invasie in de tong door middel van multi-foton microscopie van gelabelde cellen wordt gepresenteerd. Dit systeem kan dienen als een nuttig platform voor de moleculaire beoordeling en werkzaamheid van het geneesmiddel van anti-invasieve verbindingen.

Multi-foton Imaging van de tumorcel invasie in een orthotope Muis Model van Oral plaveiselcelcarcinoom

In this video, we develop an orthotopic head and neck cancer murine model by intramucosal inoculation of the squamous cell carcinoma cell line. The tumor-bearing mouse would show resemblance to clinically relevant symptoms of human disease and can thus be used to study cancer progression and discover novel therapeutic strategies.

Intramucosal Inoculation of Cancer Cells in Murine Model: A Procedure to Establish an Orthotopic Head and Neck Cancer Mouse Model

Source: Alomari, S., et al. Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection. J. Vis. Exp. (2022)This video demonstrates the use of a minimally invasive resection system (MIRS) to excise tumor tissue from an anesthetized mouse implanted with a tumor. The procedure involves securing the mouse onto a stereotactic frame, reopening the previous surgical site to access the brain, and inserting a specialized cannula that utilizes suction and a...

Brain Tumor Resection in a Mouse Using a Minimally Invasive Resection System

Preklinische modellen die adjuvante therapie beoordelen die gericht zijn op metastase van borstkanker, ontbreken. Om dit aan te pakken, ontwikkelden we een muizenmodel van de novo pulmonale mammary adenocarcinoma metastase, waarbij therapieën toegediend in de adjuvante omgeving (post-chirurgische resectie van primaire tumoren) kunnen worden geëvalueerd voor werkzaamheid bij het beïnvloeden van eerder gecoede pulmonale metastasen.

Chirurgische Procedures en Methodologie voor een Preklinisch Murine Model van De Novo Maagkanker Metastase

Implementatie van een orthotope model niercelcarcinoom in immunocompetente muizen levert de onderzoeker een klinisch relevante systeem gedefinieerd door de aanwezigheid van een primaire niertumor en longmetastasen in hetzelfde dier. Dit systeem kan worden gebruikt om een verscheidenheid aan behandelingen in vivo preklinisch testen.

Een Syngenische muismodel van gemetastaseerd niercelcarcinoom voor Kwantitatieve en Longitudinale Evaluatie van de preklinische therapieën

We beschrijven de oprichting van orthotopische colorectale tumoren via injectie van tumorcellen of organoïden in de cecum van muizen en de daaropvolgende isolatie van circulerende tumorcellen (CTC's) uit dit model.

Isolatie van circulerende tumorcellen in een orthotopische muismodel van colorectale kanker

Wij introduceren een lymfkliertest orthotopic borst kanker en radicale mastectomie model met bioluminescentie technologie te kwantificeren van de last van de tumor om na te bootsen menselijke borst kanker progressie.

Het genereren van een lymfkliertest Orthotopic uitgezaaide borst kanker Model en het uitvoeren van lymfkliertest radicale mastectomie

Hier presenteren we een reproduceerbare methode voor het ontwikkelen van een model orthotopic lymfkliertest van hoofd en nek plaveiselcelcarcinoom. Tumoren tonen klinisch relevante histopathologische kenmerken van de ziekte, met inbegrip van necrose, arme differentiatie, knooppunten metastasen en immuun infiltratie. Tumor-dragende muizen ontwikkelen klinisch relevante symptomen zoals dysfagie, verplaatsing van de kaak en gewichtsverlies.

Intramucosal enten van plaveiselcelcarcinoom cellen in muizen voor Tumor immuun profileren en evaluatie van de reactie van de behandeling

In dit protocol beschrijven we een muismodel van onvolledige chirurgische resectie van weke delen sarcoom voor het testen van (neo)adjuvante therapieën.

Een muismodel van onvolledig gereseceerde weke delen Sarcoom voor het testen van (Neo)adjuvante therapieën

In de klinische context zullen patiënten met gelokaliseerde alvleesklierkanker pancreatectomie ondergaan, gevolgd door adjuvante behandeling. Dit hier gerapporteerde protocol is bedoeld om een veilige en effectieve methode vast te stellen om dit klinische scenario bij naakte muizen te modelleren, door orthotopische implantatie van alvleesklierkanker gevolgd door distale pancreatectomie en splenectomie.

Een orthotopisch resectiemuismodel van alvleesklierkanker

Leer een orthotopisch tumorresectiemodel voor oraal plaveiselcelcarcinoom bij muizen. Deze methode gebruikt Mach Two-cellen en subtotale resectie om tumorrecidief en resistentie te bestuderen.

Translationeel relevant tumorresectiemodel voor muispreklinische modellen van orale plaveiselcelcarcinoom

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