Name: Niet-invasieve endotracheale toediening van lipopolysaccharide om acute longschade bij knaagdieren te induceren (Video) | JoVE Journal
Uploaded: 2025-12-05T12:40:00.000+00:00
Duration: 4 min 10 s
Description: Leer een niet-invasieve endotracheale instillatietechniek om acute longschade bij ratten te induceren met lipopolysaccharide. Deze methode maakt herhaalde dosering mogelijk voor onderzoek naar longherstel.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Rayees, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Macrophage+TLR4+and+PAR2+signaling%3A+Role+in+regulating+vascular+inflammatory+injury+and+repair%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Macrophage TLR4 and PAR2 signaling: Role in regulating vascular inflammatory injury and repair.</a> Front Immunol. 18 (11), 2091(2020).</li>
<li>Bernard, G. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+American-European+Consensus+Conference+on+ARDS.+Definitions%2C+mechanisms%2C+relevant+outcomes%2C+and+clinical+trial+coordination%5BTitle%5D)+AND+%22Am+J+Respir+Crit+Care+Med%22%5BJournal%5D)">The American-European Consensus Conference on ARDS. Definitions, mechanisms, relevant outcomes, and clinical trial coordination.</a> Am J Respir Crit Care Med. 149 (3), 818-824 (1994).</li>
<li>Banavasi, H., Nguyen, P., Osman, H., Soubani, A. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Management+of+ARDS+-+What+works+and+what+does+not%5BTitle%5D)+AND+%22Am+J+Med+Sci%22%5BJournal%5D)">Management of ARDS - What works and what does not.</a> Am J Med Sci. 362 (1), 13-23 (2021).</li>
<li>Bellani, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Epidemiology%2C+patterns+of+care%2C+and+mortality+for+patients+with+acute+respiratory+distress+syndrome+in+intensive+care+units+in+50+countries%5BTitle%5D)+AND+%22JAMA%22%5BJournal%5D)">Epidemiology, patterns of care, and mortality for patients with acute respiratory distress syndrome in intensive care units in 50 countries.</a> JAMA. 315 (8), 788-800 (2016).</li>
<li>Rubenfeld, G. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Incidence+and+outcomes+of+acute+lung+injury%5BTitle%5D)+AND+%22N+Engl+J+Med%22%5BJournal%5D)">Incidence and outcomes of acute lung injury.</a> N Engl J Med. 353 (16), 1685-1693 (2005).</li>
<li>Aliberti, S., Dela Cruz, C. S., Amati, F., Sotgiu, G., Restrepo, M. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Community-acquired+pneumonia%5BTitle%5D)+AND+%22Lancet%22%5BJournal%5D)">Community-acquired pneumonia.</a> Lancet. 398 (10303), 906-919 (2021).</li>
<li>Gregorakos, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Near-drowning%3A+clinical+course+of+lung+injury+in+adults%5BTitle%5D)+AND+%22Lung%22%5BJournal%5D)">Near-drowning: clinical course of lung injury in adults.</a> Lung. 187 (2), 93-97 (2009).</li>
<li>Hu, X., Lee, J. S., Pianosi, P. T., Ryu, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aspiration-related+pulmonary+syndromes%5BTitle%5D)+AND+%22Chest%22%5BJournal%5D)">Aspiration-related pulmonary syndromes.</a> Chest. 147 (3), 815-823 (2015).</li>
<li>Wang, L., Wu, D., Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chlorine+gas+inhalation+manifesting+with+severe+acute+respiratory+distress+syndrome+successfully+treated+by+high-volume+hemofiltration%3A+A+case+report%5BTitle%5D)+AND+%22Medicine%22%5BJournal%5D)">Chlorine gas inhalation manifesting with severe acute respiratory distress syndrome successfully treated by high-volume hemofiltration: A case report.</a> Medicine. 97 (30), e11708(2018).</li>
<li>Sung, P. S., Peng, Y. C., Yang, S. P., Chiu, C. H., Hsieh, S. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CLEC5A+is+critical+in+Pseudomonas+aeruginosa-induced+NET+formation+and+acute+lung+injury%5BTitle%5D)+AND+%22JCI+Insight%22%5BJournal%5D)">CLEC5A is critical in Pseudomonas aeruginosa-induced NET formation and acute lung injury.</a> JCI Insight. 7 (18), e156613(2022).</li>
<li>Al-Kuraishy, H. M., Al-Gareeb, A. I., Alblihed, M., Cruz-Martins, N., Batiha, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((COVID-19+and+risk+of+acute+ischemic+stroke+and+acute+lung+injury+in+patients+with+type+II+diabetes+mellitus%3A+The+anti-inflammatory+role+of+metformin%5BTitle%5D)+AND+%22Frontiers+Med%22%5BJournal%5D)">COVID-19 and risk of acute ischemic stroke and acute lung injury in patients with type II diabetes mellitus: The anti-inflammatory role of metformin.</a> Frontiers Med. 19 (8), 644295(2021).</li>
<li>Al-Kuraishy, H. M., Al-Gareeb, A. I., Al-Niemi, M. S., Alexiou, A., Batiha, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Calprotectin%3A+The+link+between+acute+lung+injury+and+gastrointestinal+injury+in+Covid-19%3A+Ban+or+boon%5BTitle%5D)+AND+%22Curr+Protein+Peptide+Sci%22%5BJournal%5D)">Calprotectin: The link between acute lung injury and gastrointestinal injury in Covid-19: Ban or boon.</a> Curr Protein Peptide Sci. 23 (5), 310-320 (2022).</li>
<li>Al-Kuraishy, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((COVID-19+and+phosphodiesterase+enzyme+type+5+inhibitors%5BTitle%5D)+AND+%22J+Micros+Ultrastruc%22%5BJournal%5D)">COVID-19 and phosphodiesterase enzyme type 5 inhibitors.</a> J Micros Ultrastruc. 8 (4), 141-145 (2020).</li>
<li>Al-Kuraishy, H. M., Al-Gareeb, A. I., Qusty, N., Alexiou, A., Batiha, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+sitagliptin+on+non-diabetic+COVID-19+patients%5BTitle%5D)+AND+%22Current+Mol+Pharmacol%22%5BJournal%5D)">Impact of sitagliptin on non-diabetic COVID-19 patients.</a> Current Mol Pharmacol. 15 (4), 683-692 (2022).</li>
<li>Yu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Loss+of+PADI2+and+PADI4+ameliorates+sepsis-induced+acute+lung+injury+by+suppressing+NLRP3%2B+macrophages%5BTitle%5D)+AND+%22JCI+Insight%22%5BJournal%5D)">Loss of PADI2 and PADI4 ameliorates sepsis-induced acute lung injury by suppressing NLRP3+ macrophages.</a> JCI Insight. 9 (22), e181686(2024).</li>
<li>Calfee, C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+respiratory+distress+syndrome+network%2C+national+heart%2C+lung%2C+and+blood+institute.+Trauma-associated+lung+injury+differs+clinically+and+biologically+from+acute+lung+injury+due+to+other+clinical+disorders%5BTitle%5D)+AND+%22Crit+Care+Med%22%5BJournal%5D)">Acute respiratory distress syndrome network, national heart, lung, and blood institute. Trauma-associated lung injury differs clinically and biologically from acute lung injury due to other clinical disorders.</a> Crit Care Med. 35 (10), 2243-2250 (2007).</li>
<li>Zhou, M. T., Chen, C. S., Chen, B. C., Zhang, Q. Y., Andersson, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+lung+injury+and+ARDS+in+acute+pancreatitis%3A+mechanisms+and+potential+intervention%5BTitle%5D)+AND+%22World+J+Gastroenterol%22%5BJournal%5D)">Acute lung injury and ARDS in acute pancreatitis: mechanisms and potential intervention.</a> World J Gastroenterol. 16 (17), 2094-2099 (2010).</li>
<li>Roubinian, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((TACO+and+TRALI%3A+biology%2C+risk+factors%2C+and+prevention+strategies%5BTitle%5D)+AND+%22Hematology+Am+Soc+Hematol+Educ+Program%22%5BJournal%5D)">TACO and TRALI: biology, risk factors, and prevention strategies.</a> Hematology Am Soc Hematol Educ Program. 1, 585-594 (2018).</li>
<li>Fernandes, C. A., Vanbever, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pre-clinical+models+for+pulmonary+drug+delivery%5BTitle%5D)+AND+%22Expert+Opin+Drug+Deliv%22%5BJournal%5D)">Pre-clinical models for pulmonary drug delivery.</a> Expert Opin Drug Deliv. 6 (11), 1231-1245 (2010).</li>
<li>Yu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-invasive+intratracheal+lipopolysaccharide+instillation+in+mice%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Non-invasive intratracheal lipopolysaccharide instillation in mice.</a> J Vis Exp. (193), e65151(2023).</li>
<li>Price, D. N., Muttil, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Delivery+of+therapeutics+to+the+lung%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Delivery of therapeutics to the lung.</a> Methods Mol Biol. 1809, 415-429 (2018).</li>
<li>Driscoll, K. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intratracheal+instillation+as+an+exposure+technique+for+the+evaluation+of+respiratory+tract+toxicity%3A+uses+and+limitations%5BTitle%5D)+AND+%22Toxicol+Sci%22%5BJournal%5D)">Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: uses and limitations.</a> Toxicol Sci. 55 (1), 24-35 (2000).</li>
<li>Hendrix, J. M., Regunath, H. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aHendrix%2C+JM+%22Intubation+endotracheal+tube+medications.+%5BUpdated+2025+Jan+19%5D%22">Intubation endotracheal tube medications. [Updated 2025 Jan 19]</a>. , StatPearls Publishing. Treasure Island (FL). (2025).</li>
<li>DeMasi, S. C., Casey, J. D., Semler, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evidence-based+emergency+tracheal+intubation%5BTitle%5D)+AND+%22Am+J+Respir+Crit+Care+Med%22%5BJournal%5D)">Evidence-based emergency tracheal intubation.</a> Am J Respir Crit Care Med. 211 (7), 1156-1164 (2025).</li>
<li>Greenberg, M. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+use+of+endotracheal+medication+in+cardiac+emergencies%5BTitle%5D)+AND+%22Resuscitation%22%5BJournal%5D)">The use of endotracheal medication in cardiac emergencies.</a> Resuscitation. 12 (3), 155-165 (1984).</li>
<li>Rayees, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((PAR2-Mediated+cAMP+generation+suppresses+TRPV4-dependent+Ca2%2B+signaling+in+alveolar+macrophages+to+resolve+TLR4-induced+inflammation%5BTitle%5D)+AND+%22Cell+Rep%22%5BJournal%5D)">PAR2-Mediated cAMP generation suppresses TRPV4-dependent Ca2+ signaling in alveolar macrophages to resolve TLR4-induced inflammation.</a> Cell Rep. 27 (3), 793-805 (2019).</li>
<li>Arfath, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((TRPV4+facilitates+the+reprogramming+of+inflamed+macrophages+by+regulating+IL-10+production+via+CREB%5BTitle%5D)+AND+%22Inflamm+Res%22%5BJournal%5D)">TRPV4 facilitates the reprogramming of inflamed macrophages by regulating IL-10 production via CREB.</a> Inflamm Res. 73 (10), 1687-1697 (2024).</li>
<li>Rochford, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evidence+for+reprogramming+of+monocytes+into+reparative+alveolar+macrophages+in+vivo+by+targeting+PDE4b%5BTitle%5D)+AND+%22Am+J+Physiol+Lung+Cell+Mol+Physiol%22%5BJournal%5D)">Evidence for reprogramming of monocytes into reparative alveolar macrophages in vivo by targeting PDE4b.</a> Am J Physiol Lung Cell Mol Physiol. 321 (4), L686-L702 (2021).</li>
<li>Liu, F., Li, W., Pauluhn, J., Trübel, H., Wang, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lipopolysaccharide-induced+acute+lung+injury+in+rats%3A+comparative+assessment+of+intratracheal+instillation+and+aerosol+inhalation%5BTitle%5D)+AND+%22Toxicology%22%5BJournal%5D)">Lipopolysaccharide-induced acute lung injury in rats: comparative assessment of intratracheal instillation and aerosol inhalation.</a> Toxicology. 8 (304), 158-166 (2013).</li>
<li>Hagawane, T. N., Gaikwad, R. V., Kshirsagar, N. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dual+hit+lipopolysaccharide+and+oleic+acid+combination+induced+rat+model+of+acute+lung+injury%2Facute+respiratory+distress+syndrome%5BTitle%5D)+AND+%22J+Med+Res%22%5BJournal%5D)">Dual hit lipopolysaccharide and oleic acid combination induced rat model of acute lung injury/acute respiratory distress syndrome.</a> J Med Res. 143 (5), 624-632 (2016).</li>
<li>Rayees, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protease-activated+receptor+2+promotes+clearance+of+Pseudomonas+aeruginosa+infection+by+inducing+cAMP-Rac1+signaling+in+alveolar+macrophages%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">Protease-activated receptor 2 promotes clearance of Pseudomonas aeruginosa infection by inducing cAMP-Rac1 signaling in alveolar macrophages.</a> Front Pharmacol. 20 (13), 874197(2022).</li>
<li>Rayees, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Therapeutic+effects+of+R8%2C+a+semi-synthetic+analogue+of+Vasicine%2C+on+a+murine+model+of+allergic+airway+inflammation+via+STAT6+inhibition%5BTitle%5D)+AND+%22Int+Immunopharmacol%22%5BJournal%5D)">Therapeutic effects of R8, a semi-synthetic analogue of Vasicine, on a murine model of allergic airway inflammation via STAT6 inhibition.</a> Int Immunopharmacol. 26 (1), 246-256 (2015).</li>
<li>Joshi, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((SPHK2-Generated+S1P+in+CD11b%2B+Macrophages+blocks+STING+to+suppress+the+inflammatory+function+of+alveolar+macrophages%5BTitle%5D)+AND+%22Cell+Rep%22%5BJournal%5D)">SPHK2-Generated S1P in CD11b+ Macrophages blocks STING to suppress the inflammatory function of alveolar macrophages.</a> Cell Rep. 30 (12), 4096-4109 (2020).</li>
<li>Balzer, C., Cleveland, W. J., Jinka, T. R., Riess, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Video+laryngoscopic+oral+intubation+in+rats%3A+A+simple+and+effective+method%5BTitle%5D)+AND+%22Am+J+Physiol+Lung+Cell+Mol+Physiol%22%5BJournal%5D)">Video laryngoscopic oral intubation in rats: A simple and effective method.</a> Am J Physiol Lung Cell Mol Physiol. 318 (5), L1032-L1035 (2020).</li>
</ol>

<table><tbody><tr><td>&nbsp;Prism 8</td><td>Graphpad</td><td>https://www.graphpad.com/features</td><td></td></tr><tr><td>Bradford Reagens&nbsp;</td><td>Himedia, India</td><td>ML106-500 ML&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</td><td></td></tr><tr><td>DL-Dithiothreitol</td><td>Himedia, India&nbsp;</td><td>RM525-25G</td><td></td></tr><tr><td>Eosine&nbsp;</td><td>Loba Chemie, India</td><td>3660</td><td></td></tr><tr><td>Ethyleen Diamine Tetra Acetic Acid (EDTA)</td><td>Loba Chemie, India</td><td>6381926</td><td></td></tr><tr><td>Forceps&nbsp;</td><td></td><td>-</td><td></td></tr><tr><td>Formaline 10%&nbsp;</td><td>Sigma Aldrich, USA&nbsp;</td><td>HT501320</td><td></td></tr><tr><td>Hematoxyline</td><td>SDFCL</td><td>74108G05</td><td></td></tr><tr><td>HEPES</td><td>Himedia, India</td><td>MB016</td><td></td></tr><tr><td>Waterstofperoxide&nbsp;&nbsp;</td><td>Avra, India</td><td>7722841</td><td></td></tr><tr><td>Ketamine&nbsp;</td><td>Neon Laboratories Ltd, India</td><td>-&nbsp;</td><td></td></tr><tr><td>Laryngoscope</td><td>Medical Sources Co., Ltd., China&nbsp;</td><td>MS-LA500FV&nbsp;</td><td></td></tr><tr><td>Lipopolysaccharide van Escherichia coli O55:B5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</td><td>Sigma Aldrich, USA</td><td>L2880&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</td><td></td></tr><tr><td>o-Dianisidine dihydrochloride&nbsp;</td><td>Sigma Aldrich, USA</td><td>D3252-5G&nbsp;</td><td></td></tr><tr><td>Fosfaat Buffered Saline (PBS)&nbsp;</td><td>Invitrogen, USA</td><td>3002</td><td></td></tr><tr><td>Kaliumdiwaterstoffosfaat</td><td>Himedia, India</td><td>IN26185530500</td><td></td></tr><tr><td>Proteïne inhibitor cocktail</td><td>Sigma Aldrich</td><td>P8340</td><td></td></tr><tr><td>Rat IL-1&beta; ELISA Kit&nbsp;</td><td>Thermofisher, USA</td><td>BMS630</td><td></td></tr><tr><td>Natriumchloride</td><td>Himedia, India</td><td>TC046M</td><td></td></tr><tr><td>Natriumfluoride</td><td>Himedia, India</td><td>GRM7502</td><td></td></tr><tr><td>Natriumorthovanadate</td><td>Sigma Aldrich, USA</td><td>S6508</td><td></td></tr><tr><td>Natriumpyrofosfaat</td><td>Merck, USA</td><td>MA3M722243</td><td></td></tr><tr><td>Zwavelzuur</td><td>Sigma Aldrich, USA</td><td>339741</td><td></td></tr><tr><td>Triton X-100</td><td>Thermofisher, USA</td><td>T8787</td><td></td></tr><tr><td>Xylazijn&nbsp;</td><td>Indian Immunologicals Ltd, India</td><td>-</td><td></td></tr></tbody></table>

niet-invasieve endotracheale toediening van lipopolysaccharide om acute longschade bij knaagdieren te induceren

Non-Invasive Endotracheal Administration of Lipopolysaccharide to Induce Acute Lung Injury in Rodents

Niet-invasieve endotracheale toediening van lipopolysaccharide om acute longschade bij knaagdieren te induceren

Acute Lung Injury (ALI) is a life-threatening inflammatory condition characterized by arterial hypoxemia, alveolar edema, compromised ...

non-invasive-endotracheal-administration-lipopolysaccharide-to-induce

Research

JoVE Journal

Biology

Biologie

Een muismodel voor de ventilator geïnduceerde longschade is een belangrijk instrument om een ​​acute longbeschadiging...

Druk gecontroleerde ventilatie te Acute Lung Injury in Muizen Braken

Source: Radulovic, K., et al. Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach. J. Vis. Exp. (2018)This video demonstrates a step-by-step procedure for injecting lipopolysaccharide (LPS) into mice intraperitoneally to induce systemic inflammation.

Intraperitoneal Injection of Bacterial Lipopolysaccharide in Mice to Study Systemic Immune Responses

Source: Yuan, Z., et al. Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury. J. Vis. Exp. (2018)This video demonstrates an in vivo mouse model for inducing and analyzing acute lung injury (ALI) using intraperitoneal administration of bacterial lipopolysaccharide. The inflammatory cascade triggered by alveolar macrophages and neutrophils leads to epithelial and endothelial barrier disruption, allowing fluid and immune cells to accumulate in the alveoli. Alveolar...

Lipopolysaccharide-Induced Acute Lung Injury in Mice for Exosome and Immune Analysis

De gastheer immuunrespons op pathogene infectie een strak gereguleerd proces. Met behulp van een lipopolysaccharide long exposure model in muizen, is het mogelijk om hoge resolutie evaluaties van de complexe mechanismen van pathogenese voeren.

Het gebruik van Oropharyngeal Intratracheaal PAMP Administratie en bronchoalveolaire lavage om de immuunrespons van de gastheer in Muizen Evalueer

We beschrijven een werkwijze voor het induceren neutrofiele longontsteking door uitdaging om vernevelde lipopolysacharide door verneveling, te modelleren acute longbeschadiging. Daarnaast worden fundamentele chirurgische technieken long isolatie, tracheale intubatie en bronchoalveolaire lavage beschreven.

Een methode voor het genereren van Pulmonale neutrofilie behulp Aerosolized Lipopolysaccharide

Dit protocol induceert acute longschade bij een muis met een hoge nauwkeurigheid die vergelijkbaar is met de pathogenese van acide pneumonitis die bij mensen wordt waargenomen. We genereren een maximale acute niet-dodelijke lage pH-longschade en houden rekening met verschillen in de anatomische ademhalingsstructuur van knaagdieren-mensen door middel van een open tracheale opening gekoppeld aan circumferentiële drukontlasting.

Open Tracheostomie Maagzuur Aspiration muizenmodel van Acute Lung Injury Resultaten in Maximal Acute Lung Injury nonlethal

Hier gepresenteerd is een stap-voor-stap procedure om acute longschade in muizen induceren door directe intratracheal lipopolysaccharide instillatie en uit te voeren FACS analyse van bloedmonsters, bronchoalveolaire lavage vloeistof, en longweefsel. Minimale invasieve, eenvoudige handling, goede reproduceerbaarheid, en titratie van de ziekte ernst zijn voordelen van deze aanpak.

Inducerende acute Long blessure bij muizen door directe Intratracheal lipopolysaccharide instillatie

Gecombineerde ozon- en bacteriële endotoxine blootgestelde muizen vertonen wijdverspreide celdood, waaronder die van neutrofielen. We zagen cellulaire aanpassingen zoals verstoring van cytoskelet lamellipodie, verhoogde cellulaire expressie van complexe V ATP synthase subeenheid β en angiostatine in broncho-alveolaire lavage, onderdrukking van de immuunrespons van de longen en vertraagde neutrofiele rekrutering.

Visualiseren van long cellulaire aanpassingen tijdens gecombineerde ozon en LPS geïnduceerde murine acute longletsel

Hier stellen we een protocol voor intratracheale lipopolysaccharide (LPS) levering via niet-invasieve orofaryngeale endotracheale intubatie. Deze methode minimaliseert het trauma van de chirurgische procedure voor het dier en levert LPS nauwkeurig af aan de luchtpijp en vervolgens aan de longen.

Niet-invasieve intratracheale lipopolysaccharide-instillatie bij muizen

We bieden een protocol voor een experimenteel endotoxemisch shockmodel bij varkens door infusie van lipopolysaccharide.

Lipopolysaccharide-infusie als een endotoxemisch shockmodel bij varkens

Dit protocol beschrijft de ontwikkeling van een neonataal biggenmodel van acuut longletsel dat vroege pathogene gebeurtenissen modelleert die optreden in de premature long, waaronder een subadequate hoeveelheid oppervlakteactieve stoffen, hyperoxie, hogedrukventilatie en ontsteking, om het begrip van moleculaire triggers van bronchopulmonale dysplasie te vergemakkelijken en de therapeutische vertaling te verbeteren.

Ontwikkeling van een model voor acuut longletsel bij neonatale biggen dat de vroege omgeving van te vroeg geboren longen nabootst

Leer een niet-invasieve endotracheale instillatietechniek om acute longschade bij ratten te induceren met lipopolysaccharide. Deze methode maakt herhaalde dosering mogelijk voor onderzoek naar longherstel.

Niet-invasieve endotracheale toediening van lipopolysaccharide om acute longschade bij knaagdieren te induceren

Meer video's verkennen