Name: Verbetering van chimere antigeenreceptor-extracellulaire blaasjes (CAR-EV) -technologie: de toekomst van kankertherapie (Video) | JoVE Journal
Uploaded: 2025-09-19T13:20:00.000+00:00
Duration: 7 min 33 s
Description: Ontdek de ontwikkeling van CAR-EV-technologie voor gerichte kankertherapie met verbeterde isolatiemethoden. Deze innovatieve aanpak levert veilige, effectieve celvrije behandelingen voor solide tumoren.

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<li>Bachurski, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extracellular+vesicle+measurements+with+nanoparticle+tracking+analysis-an+accuracy+and+repeatability+comparison+between+NanoSight+NS300+and+ZetaView%5BTitle%5D)+AND+%22JEV%22%5BJournal%5D)">Extracellular vesicle measurements with nanoparticle tracking analysis-an accuracy and repeatability comparison between NanoSight NS300 and ZetaView.</a> JEV. 8 (1), 1596016(2019).</li>
<li>Khanabdali, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+throughput+surface+epitope+immunoaffinity+isolation+of+extracellular+vesicles+and+downstream+analysis%5BTitle%5D)+AND+%22Biol+Methods+Protoc%22%5BJournal%5D)">High throughput surface epitope immunoaffinity isolation of extracellular vesicles and downstream analysis.</a> Biol Methods Protoc. 9 (1), bpae032(2024).</li>
<li>Salomon, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Early+detection+of+ovarian+cancer%3A+An+accurate+high-throughput+extracellular+vesicle+test%5BTitle%5D)+AND+%22J+Clin+Oncol%22%5BJournal%5D)">Early detection of ovarian cancer: An accurate high-throughput extracellular vesicle test.</a> J Clin Oncol. 43 (16_suppl), 5582-5582 (2025).</li>
<li>Fu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CAR+exosomes+derived+from+effector+CAR-T+cells+have+potent+antitumour+effects+and+low+toxicity%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity.</a> Nat Commun. 10 (1), 1-12 (2019).</li>
<li>Zheng, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhalable+CAR-T+cell-derived+exosomes+as+paclitaxel+carriers+for+treating+lung+cancer%5BTitle%5D)+AND+%22J+Transl+Med%22%5BJournal%5D)">Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer.</a> J Transl Med. 21 (1), 383(2023).</li>
<li>Tian, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Engineered+exosome+for+drug+delivery%3A+Recent+development+and+clinical+applications%5BTitle%5D)+AND+%22Int+J+Nanomed%22%5BJournal%5D)">Engineered exosome for drug delivery: Recent development and clinical applications.</a> Int J Nanomed. 18, 7923-7940 (2023).</li>
<li>Chen, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Encapsulation+and+assessment+of+therapeutic+cargo+in+engineered+exosomes%3Aa+systematic+review%5BTitle%5D)+AND+%22J+Nanobiotechnology%22%5BJournal%5D)">Encapsulation and assessment of therapeutic cargo in engineered exosomes:a systematic review.</a> J Nanobiotechnology. 22 (1), 18(2024).</li>
<li>Basyoni, A. E., Atta, A., Salem, M. M., Mohamed, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Harnessing+exosomes+for+targeted+drug+delivery+systems+to+combat+brain+cancer%5BTitle%5D)+AND+%22Cancer+Cell+Int%22%5BJournal%5D)">Harnessing exosomes for targeted drug delivery systems to combat brain cancer.</a> Cancer Cell Int. 25 (1), 150(2025).</li>
<li>Coughlan, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exosome+isolation+by+ultracentrifugation+and+precipitation+and+techniques+for+downstream+analyses%5BTitle%5D)+AND+%22Curr+Protoc+Cell+Biol%22%5BJournal%5D)">Exosome isolation by ultracentrifugation and precipitation and techniques for downstream analyses.</a> Curr Protoc Cell Biol. 88 (1), e110(2020).</li>
<li>Zhang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Large-scale+separation+and+purification+of+exosomes+using+ion-exchange+chromatography%5BTitle%5D)+AND+%22Biomed+Res+Ther%22%5BJournal%5D)">Large-scale separation and purification of exosomes using ion-exchange chromatography.</a> Biomed Res Ther. 11 (4), 6348-6356 (2024).</li>
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<li>Dobersberger, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+engineering+strategy+to+target+activated+EGFR+with+CAR+T+cells%5BTitle%5D)+AND+%22Cell+Rep+Methods%22%5BJournal%5D)">An engineering strategy to target activated EGFR with CAR T cells.</a> Cell Rep Methods. 4 (4), 100728-100728 (2024).</li>
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</ol>

<table><tbody><tr><td>Materials</td><td></td><td></td><td></td></tr><tr><td>1x phosphate buffered saline (PBS), pH 7.4</td><td>Gibco</td><td>10010049</td><td>EV verrijking</td></tr><tr><td>1% sodium dodecyl sulfate (SDS)</td><td>Sigma</td><td>L3771-100G</td><td>Los 1g SDS poeder op in 10 mL ultrapuur water</td></tr><tr><td>1x tris buffered saline met Tween-20 (TBST)</td><td>Thermo Scientific Chemicals</td><td>J77500.K2</td><td>Verdun met ultrapuur water tot een 1x oplossing (alternatief, bereid een 0,1% polysorbaat-20/Tween-20 oplossing in 1x tris buffered saline)</td></tr><tr><td>96-wells heldere platte bodem polystyreen TC-behandelde microplaat, steriele</td><td>Corning</td><td>3595</td><td>In vitro functioneel assay</td></tr><tr><td>Bolt Bis-Tris Plus Mini Protein Gels, 4-12%, 1,0 mm, WedgeWell formaat</td><td>Invitrogen</td><td></td><td>Cel/EV karakterisering</td></tr><tr><td>Bovien serum albumine</td><td>Sigma-Aldrich</td><td>A9647-100G</td><td>Cel/EV karakterisering</td></tr><tr><td>Calnexin antilichaam</td><td>Cell Signaling</td><td>2433S</td><td>Cel/EV karakterisering</td></tr><tr><td>CAR-NK/T cellen</td><td>ProMab Biotechnologies</td><td>Varieert</td><td>CAR extracellulaire ligand-bindende domein hangt af van de voorkeursdoelwit op recipient cellen</td></tr><tr><td>CellTiter 96 AQueous One Solution Cel Proliferatie Assay (MTS)</td><td>Promega</td><td>G3580</td><td>In vitro functioneel assay</td></tr><tr><td>cOmplete, Mini, EDTA-vrije Protease Inhibitor Cocktail</td><td>Roche</td><td>11836170001</td><td>Cel lysis</td></tr><tr><td>ECL Western blotting detectiereagens</td><td>Amersham</td><td>RPN2232</td><td>Cel/EV karakterisering</td></tr><tr><td>Elutie buffer</td><td>INOVIQ</td><td></td><td>EV verrijking/isolatie</td></tr><tr><td>Equilibratie buffer</td><td>INOVIQ</td><td></td><td>EV verrijking/isolatie</td></tr><tr><td>EXO-ACE IEX kolom</td><td>INOVIQ</td><td></td><td>EV verrijking/isolatie voor downstream functionele assay Patent-ID AU2024901931</td></tr><tr><td>EXO-NET pan-exosome opvangkralen</td><td>INOVIQ</td><td></td><td>EV isolatie voor downstream karakterisering Patent-ID WO/2014/011673</td></tr><tr><td>Granzyme B antilichaam</td><td>Cell Signaling</td><td>4275</td><td>Cel/EV karakterisering</td></tr><tr><td>Human IL-2 IS, premium grade</td><td>Miltenyi Biotec</td><td>130-097-744</td><td>Xeno-vrije cultuur van T/NK cellen</td></tr><tr><td>iBlot 2 Transfer Stacks, PVDF</td><td>Invitrogen</td><td></td><td>Cel/EV karakterisering</td></tr><tr><td>ImmunoCult-XF (cGMP)</td><td>Stemcell Technologies</td><td>100-0956</td><td>Xeno-vrije cultuur van T/NK cellen</td></tr><tr><td>K-562</td><td>ATCC</td><td>CCL-243</td><td>In vitro functioneel assay</td></tr><tr><td>MCF-7</td><td>ATCC</td><td>HTB-22</td><td>In vitro functioneel assay</td></tr><tr><td>Millex-GP spuitfilterunit, 0,22 &micro;m</td><td>Merck Millipore</td><td>SLGPR33RS</td><td>Terminale filtratie</td></tr><tr><td>Regeneratie buffer</td><td>INOVIQ</td><td></td><td>EV verrijking/isolatie</td></tr><tr><td>RIPA buffer (10X)</td><td>Cell Signaling Technology</td><td>9806</td><td>Cel lysis</td></tr><tr><td>Trypan blauw oplossing, 0,4%</td><td>Gibco</td><td>15250061</td><td>Beoordeling van cellevensvatbaarheid en dichtheid</td></tr><tr><td>Wash buffer</td><td>INOVIQ</td><td></td><td>EV verrijking/isolatie</td></tr><tr><td>Equipment</td><td></td><td></td><td></td></tr><tr><td>Automatische celteller</td><td>Invitrogen</td><td>Countess II FL</td><td>Beoordeling van cellevensvatbaarheid en dichtheid</td></tr><tr><td>Benchtop geautomatiseerd extractie-instrument</td><td>Invitrogen</td><td>KingFisher Apex System</td><td>Hoge doorvoer geautomatiseerd zuiveringssysteem Zie link voor protocol details: https://www.inoviq.com/site/technology/resources</td></tr><tr><td>Fluorescentiemicroscoop</td><td>Zeiss</td><td>Axio Observer 7</td><td>Cel cultuur</td></tr><tr><td>Nanoparticle tracking analyse (NTA)</td><td>Particle Metrix GmbH</td><td>Zetaview PMX-130</td><td>Deeltjesanalyse</td></tr><tr><td>Plate reader</td><td>BMG Labtech</td><td>SPECTROstar Nano</td><td>In vitro functioneel assay</td></tr><tr><td>Gekoelde benchtop centrifuge</td><td>Beckman Coulter</td><td>Avanti J-15R</td><td>Cel cultuur & EV verrijking verwerking</td></tr><tr><td>SDS-PAGE mini gel tank</td><td>Invitrogen</td><td>A25977</td><td>Cel/EV karakterisering</td></tr><tr><td>Western blot transfer systeem</td><td>Invitrogen</td><td>iBlot 2</td><td>Cel/EV karakterisering</td></tr></tbody></table>

verbetering van chimere antigeenreceptor-extracellulaire blaasjes (car-ev) -technologie: de toekomst van kankertherapie

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Ontdek de ontwikkeling van CAR-EV-technologie voor gerichte kankertherapie met verbeterde isolatiemethoden. Deze innovatieve aanpak levert veilige, effectieve celvrije behandelingen voor solide tumoren.

Verbetering van chimere antigeenreceptor-extracellulaire blaasjes (CAR-EV) -technologie: de toekomst van kankertherapie

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