Name: TD-DFT Geleide geavanceerde E-Eye Sensing-techniek voor kwantificering ter plaatse van Fe, Cr, F en As in de milieu-, biologische en voedselmonsters (Video) | JoVE Journal
Uploaded: 2025-09-19T13:50:00.000+00:00
Duration: 9 min 51 s
Description: Leer de e-Eye-sensortechniek voor kwantificering van Fe, Cr, F en As in milieu-, biologische en voedselmonsters. Dit draagbare apparaat detecteert toxische stoffen met resultaten die overeenkomen met goudstandaardmethoden.

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</ol>

<table><tbody><tr><td>1,10-phenanthroline monohydrate (C12H8N2.H2O)</td><td>5144-89-8</td><td>Sigma Aldrich, India</td><td></td></tr><tr><td>1,5-diphenylcarbazide ( C13H14N4O)</td><td>140-22-7</td><td>Sigma Aldrich, India</td><td></td></tr><tr><td>Acetone (C3H6O)</td><td>67-64-1</td><td>Sigma Aldrich, India</td><td></td></tr><tr><td>Ammonium molybdate tetrahydrate ((NH?)?Mo?O??&middot;4H?O)</td><td>12054-85-2</td><td>Sigma Aldrich, India</td><td></td></tr><tr><td>Arduino UNO ontwikkelingsbord</td><td>SKU&nbsp;A000066</td><td>Robu.in</td><td></td></tr><tr><td>Ascorbic acid (C6H8O6)</td><td>50-81-7&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Breadboard</td><td>SKU: 24441</td><td>Robu.in</td><td></td></tr><tr><td>Ferric chloride (FeCL3)</td><td>7705-08-0&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Ferrous sulphate heptahydrate (FeSO4.7H2O)</td><td>7782-63-0&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Hydrochloric acid (HCl)</td><td>7647-01-0&nbsp;</td><td>Finar, India</td><td></td></tr><tr><td>Hydrogen peroxide (H2O2)</td><td>7722-84-1&nbsp;</td><td>Finar, India</td><td></td></tr><tr><td>Jumper wires</td><td>SKU: 44281</td><td>Robu.in</td><td></td></tr><tr><td>Light emitting diode (LED)</td><td>SKU: R110553</td><td>Robu.in</td><td></td></tr><tr><td>Light-dependent resistor (LDR)</td><td>SKU: 1496181</td><td>Robu.in</td><td></td></tr><tr><td>Liquid crystal display (LCD)</td><td>SKU: 11497</td><td>Robu.in</td><td></td></tr><tr><td>Milli-Q water (18.2&nbsp; M&Omega;)&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Multimeter</td><td>SKU: 1720374</td><td>Robu.in</td><td></td></tr><tr><td>Nitric acid (HNO3)</td><td>7697-37-2&nbsp;</td><td>Finar, India</td><td></td></tr><tr><td>Phosphate Buffer Saline (PBS)</td><td>SKU:&nbsp;M1866</td><td>Himedia, India</td><td></td></tr><tr><td>Potassium dichromate (K2Cr2O7)</td><td>7778-50-9&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Potentiometer</td><td>SKU: 790452</td><td>Robu.in</td><td></td></tr><tr><td>Resistors</td><td>SKU: R225238</td><td>Robu.in</td><td></td></tr><tr><td>Sodium arsenate heptahydrate (Na2HAsO4&middot;7H2O)</td><td>10048-95-0&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Sodium dihydrogen phosphate (NaH2PO4)&nbsp;</td><td>13472-35-0&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Sodium fluoride (NaF)</td><td>7681-49-4</td><td>Himedia, India</td><td></td></tr><tr><td>Sodium thiocyanate (NaSCN)</td><td>540-72-7&nbsp;</td><td>Himedia, India</td><td></td></tr><tr><td>Sulfuric acid (H2SO4)</td><td>7664-93-9&nbsp;</td><td>Finar, India</td><td></td></tr><tr><td>Trisodium citrate dihydrate (Na3C6H5O7.2H2O)</td><td>03-04-6132</td><td>Himedia, India</td><td></td></tr></tbody></table>

td-dft geleide geavanceerde e-eye sensing-techniek voor kwantificering ter plaatse van fe, cr, f en as in de milieu-, biologische en voedselmonsters

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Geleide geavanceerde E-Eye Sensing-techniek voor kwantificering ter plaatse van Fe, Cr, F en As in de milieu-, biologische en voedselmonsters

This paper demonstrates the step-by-step protocol of designing and developing an E-Eye-enabled multiplexed point-of-care testing (POCT) kit. We use ...

td-dft-guided-advanced-e-eye-sensing-technique-for-on-site

Research

JoVE Journal

Engineering

Techniek

Beschrijven we een procedure voor de opsporing van chemische elementen aanwezig in situ in menselijke cellen, alsmede hun kwantificering in vitro . De methode is geschikt voor elk celtype en is vooral nuttig voor de kwantitatieve chemische analyses in afzonderlijke cellen na in vitro metaaloxide nanodeeltjes blootstelling.

In Situ Detectie en eencellige kwantificering van metaaloxide nanodeeltjes met behulp van nucleaire Microprobe analyse

Dit artikel geeft een gedetailleerde werkwijze om de microstructuur van ultrafijne korrel en nanokristallijne materialen met een scanning elektronenmicroscoop uitgerust met een standaard elektronen backscatter diffractie systeem karakteriseren. Metaallegeringen en mineralen presenteren verfijnde microstructuren worden geanalyseerd met deze techniek, die de diversiteit van de toepassingsmogelijkheden.

Karakterisering van Ultra-fijnkorrelige en nanokristallijne materialen met behulp van Transmission Kikuchi Diffraction

Deze paper biedt een beknopt overzicht van de inspanningen bij de Army Research Laboratory op de verwerking van bulk nanocrystalline metalen met een nadruk op de methoden voor de productie van de roman metaalpoeders.

Verwerking van Bulk Nanocrystalline metalen bij de US Army Research Laboratory

We beschrijven de instelling van een beamline bedoeld voor het uitvoeren van snelle twee-dimensionale röntgenfoto fluorescentie en x-ray microdiffraction toewijzing van kristallen of poeder monsters met Laue (polychromatische straling) of poeder (monochromatische straling) diffractie. De resulterende kaarten geven informatie over stam, oriëntatie, fase distributie en plastische vervorming.

Synchrotron X-ray Microdiffraction en fluorescentie beeldvorming van minerale en Rock monsters

We presenteren een assay voor eenvoudige kwantificering van metalen geïntroduceerd voor monsters bereid met behulp van geïmmobiliseerde metaal affiniteit chromatografie. De methode maakt gebruik van hydroxynaphthol blauw als de colorimetrische metalen indicator en een UV-VIS-spectrofotometer als de detector.

Kwantificering van metaal uitspoeling in geïmmobiliseerde metaal Affiniteits chromatografie

Dit protocol presenteert een gedetailleerde procedure om biologische cryosamples voor synchrotron-gebaseerde röntgenabsorptiespectroscopie-experimenten voor te bereiden. We beschrijven alle stappen die nodig zijn om de monstervoorbereiding en cryopreservatie te optimaliseren met voorbeelden van het protocol met kanker- en fytoplanktoncellen. Deze methode biedt een universele standaard voor cryo-voorbereiding van monsters.

Biologische monsters voorbereiding voor soortvorming bij cryogene temperatuur met behulp van hoge-resolutie röntgenabsorptiespectroscopie

Deze ijzerrorox speciatie methode is gebaseerd op capillaire elektroforese-inductief gekoppeld plasma massaspectrometrie met monster stapelen in combinatie met korte analyse in een run. De methode analyseert en biedt snel lage limieten van kwantificering voor ijzerredoxsoorten in een breed scala van weefsels en biofluïde monsters.

Opstelling van capillaire elektroforese-inductief gekoppelde plasmamassaspectrometrie (CE-ICP-MS) voor kwantificering van ijzerrederoxsoorten (Fe(II), Fe(III))

Dit protocol presenteert een workflow voor sub-mm 2D visualisatie van meerdere labiele anorganische nutriënten- en verontreinigingsolute soorten met behulp van diffusieve gradiënten in dunne films (DGT) in combinatie met massaspectrometrie beeldvorming. Solutebemonstering en chemische analyse in hoge resolutie worden in detail beschreven voor het kwantitatief in kaart brengen van soluten in de rhizosfeer van terrestrische planten.

Tweedimensionale visualisatie en kwantificering van labiele, anorganische plantennutriënten en verontreinigingen in de bodem

We bieden een algemeen overzicht van kwantitatieve microanalysemethoden voor het schatten van de locatiebezetting van onzuiverheden en hun chemische toestanden door gebruik te maken van elektronengeangaliseerde verschijnselen onder incidentele elektronenstraal-schommelomstandigheden, die betrouwbaar informatie extraheren uit minderheidssoorten, lichtelementen, zuurstofvacatures en andere punt/lijn/planaire defecten.

Kwantitatieve atomaire-site analyse van functionele dopants/puntdefecten in kristallijne materialen door elektronengeankan-verbeterde microanalyse

Hier presenteren we een protocol om machine-vision-software te gebruiken om dynamische processen tijdens TEM-beeldvorming te stabiliseren, terwijl tegelijkertijd meerdere stromen metadata naar elke afbeelding worden geïndexeerd in een navigeerbare tijdlijn. We laten zien hoe dit platform geautomatiseerde kalibratie en mapping van de elektronendosis in de loop van een experiment mogelijk maakt.

Een machine-vision benadering van transmissie-elektronenmicroscopie workflows, resultatenanalyse en gegevensbeheer

Leer de e-Eye-sensortechniek voor kwantificering van Fe, Cr, F en As in milieu-, biologische en voedselmonsters. Dit draagbare apparaat detecteert toxische stoffen met resultaten die overeenkomen met goudstandaardmethoden.

TD-DFT Geleide geavanceerde E-Eye Sensing-techniek voor kwantificering ter plaatse van Fe, Cr, F en As in de milieu-, biologische en voedselmonsters (Video) | JoVE Journal

TD-DFT Geleide geavanceerde E-Eye Sensing-techniek voor kwantificering ter plaatse van Fe, Cr, F en As in de milieu-, biologische en voedselmonsters

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