Name: On-site DNA-detectie van trypanosomatide parasieten en Nosema ceranae door middel van alkalische lysis gekoppeld aan RPA/CRISPR/Cas12a-systeem (Video) | JoVE Journal
Uploaded: 2025-07-18T14:50:00.000+00:00
Duration: 7 min 46 s
Description: Leer een snelle DNA-detectiemethode voor bijenpathogenen zoals Lotmaria passim en Nosema ceranae met alkalische lysis gekoppeld aan RPA/CRISPR/Cas12a. Dit veldgeschikte systeem biedt hogere gevoeligheid dan QPCR.

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</ol>

<a href="https://app.jove.com/t/6641">This corrects</a> the article <a href="https://dx.doi.org/10.3791/68871">10.3791/68874</a>

An erratum was issued for:&#160;<a href="https://www.jove.com/t/68874">On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System</a>. The Authors&#160;section was&#160;updated.

Erratum: On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

<table><tbody><tr><td>0.2 mL DNAse- en RNAse-vrije Eppendorf buisjes</td><td>Axygen</td><td>MTC-02-C</td><td></td></tr><tr><td>1.5 mL DNAse- en RNAse-vrije Eppendorf buisjes</td><td>Axygen</td><td>MTC-150-C</td><td></td></tr><tr><td>10 μL lagedempering gefilterde tips</td><td>LabClinics</td><td>5-063-P5-0</td><td></td></tr><tr><td>100 μL lagedempering gefilterde tips</td><td>LabClinics</td><td>5-120-P5-0</td><td></td></tr><tr><td>1000 μL lagedempering gefilterde tips</td><td>LabClinics</td><td>5-203-P4-0</td><td></td></tr><tr><td>BH-200 Series Stuart Dry Block Heater</td><td>Cole-Parmer</td><td>EW-36610-00</td><td></td></tr><tr><td>DEPC-behandeld water</td><td>Ambicon</td><td>AM9906</td><td></td></tr><tr><td>EDTA</td><td>Sigma-Aldrich</td><td>798681</td><td></td></tr><tr><td>EnGen Lba Cas12a (Cpf1)</td><td>New England Biolabs</td><td>M0653T</td><td></td></tr><tr><td>Sigma-Aldrich</td><td>Sigma-Aldrich</td><td>64-17-5</td><td></td></tr><tr><td>HCl</td><td>Sigma-Aldrich</td><td>7647-01-0</td><td></td></tr><tr><td>Microtube stampers</td><td>Fisherbrand</td><td>12141364</td><td></td></tr><tr><td>Milenia GenLine HybriDetect</td><td>Milenia</td><td>MGHD 1</td><td></td></tr><tr><td>NaOH</td><td>Sigma-Aldrich</td><td>221465</td><td></td></tr><tr><td>RPA kit TwistAmp Liquid Basic</td><td>TwistAmp</td><td>&nbsp;TALQBAS01</td><td></td></tr><tr><td>Scalpel messen</td><td>Swann-Morton</td><td>201</td><td></td></tr><tr><td>Single kanaal Pipet PIPETMAN P1000L Micropipet Metal eject</td><td>Gilson</td><td>F144059M</td><td></td></tr><tr><td>Single kanaal Pipet PIPETMAN P100L Micropipet Metal eject</td><td>Gilson</td><td>F144057M</td><td></td></tr><tr><td>Single kanaal Pipet PIPETMAN P10L Micropipet Metal eject</td><td>Gilson</td><td>F144055M</td><td></td></tr><tr><td>Natriumhypochloriet</td><td></td><td></td><td></td></tr><tr><td>Touch Real-Time PCR Detectiesysteem</td><td>BioRad</td><td>CFX96</td><td></td></tr><tr><td>Tris-HCl</td><td>Sigma-Aldrich</td><td>T1503</td><td></td></tr><tr><td>TwistAmp Liquid Basic</td><td>Twist</td><td>TALQBAS01</td><td></td></tr></tbody></table>

on-site dna-detectie van trypanosomatide parasieten en nosema ceranae door middel van alkalische lysis gekoppeld aan rpa/crispr/cas12a-systeem

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

On-site DNA-detectie van trypanosomatide parasieten en Nosema ceranae door middel van alkalische lysis gekoppeld aan RPA/CRISPR/Cas12a-systeem

Early on-site detection of microscopic pathogens is essential for the effective management and health of honeybee colonies. Currently, the gold standard ...

on-site-dna-detection-trypanosomatid-parasites-nosema-ceranae-through

Research

JoVE Journal

Biology

Biologie

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De bijbehorende chromosoom val (ACT) assay is een nieuwe onpartijdige methode voor het identificeren van lange-afstands-DNA interacties. De karakterisering van de lange reeks DNA-interacties zal ons toelaten om de relatie van de nucleaire architectuur vast te stellen om genexpressie in zowel de normale fysiologie en in zieke staten.

Associated Chromosoom Trap voor het vaststellen van lange afstand DNA-interacties

Leer een snelle DNA-detectiemethode voor bijenpathogenen zoals Lotmaria passim en Nosema ceranae met alkalische lysis gekoppeld aan RPA/CRISPR/Cas12a. Dit veldgeschikte systeem biedt hogere gevoeligheid dan QPCR.

On-site DNA-detectie van trypanosomatide parasieten en Nosema ceranae door middel van alkalische lysis gekoppeld aan RPA/CRISPR/Cas12a-systeem (Video) | JoVE Journal

On-site DNA-detectie van trypanosomatide parasieten en Nosema ceranae door middel van alkalische lysis gekoppeld aan RPA/CRISPR/Cas12a-systeem

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