Name: Detectie van aggregatiegevoelig gedrag in mutante P53 V157F borstkankercellen met behulp van multipoint Thioflavine T-fluorescentie (Video) | JoVE Journal
Uploaded: 2025-12-30T14:57:00.000+00:00
Duration: 4 min 56 s
Description: Leer aggregatiegedrag detecteren in mutante p53 V157F borstkankercellen met multipoint Thioflavine T-fluorescentie. Deze methode verbetert de nauwkeurigheid van eiwitaggregatie-metingen in kankeronderzoek.

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<table><tbody><tr><td>96-well culture plate</td><td>Simply</td><td>PC396-0100</td><td></td></tr><tr><td>counter slide</td><td>Invitrogen</td><td>C10228</td><td></td></tr><tr><td>Countess II FL Automated Cell Counter</td><td>Thermo Fisher Scientific&nbsp;</td><td>AMQAF1000</td><td></td></tr><tr><td>dPBS</td><td>Simply</td><td>CC704-0500</td><td></td></tr><tr><td>Eppendorf tube</td><td>Simply</td><td>PC101-5000</td><td></td></tr><tr><td>Hoechst 33342</td><td>AAT Bioquest</td><td>17530</td><td></td></tr><tr><td>SpectraMax iD3</td><td>Molecular Devices</td><td>735-0391&nbsp;</td><td></td></tr><tr><td>Thioflavin T</td><td>Sigma-Aldrich</td><td>T3516</td><td></td></tr><tr><td>Trypan Blue</td><td>Invitrogen</td><td>T10282</td><td></td></tr></tbody></table>

detectie van aggregatiegevoelig gedrag in mutante p53 v157f borstkankercellen met behulp van multipoint thioflavine t-fluorescentie

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Detectie van aggregatiegevoelig gedrag in mutante P53 V157F borstkankercellen met behulp van multipoint Thioflavine T-fluorescentie

The tumor suppressor p53, encoded by the TP53 gene, plays a central role in maintaining genomic stability. TP53 mutations, particularly hotspot variants, ...

detection-aggregation-prone-behavior-mutant-p53-v157f-breast-cancer

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Kankeronderzoek

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Leer aggregatiegedrag detecteren in mutante p53 V157F borstkankercellen met multipoint Thioflavine T-fluorescentie. Deze methode verbetert de nauwkeurigheid van eiwitaggregatie-metingen in kankeronderzoek.

Detectie van aggregatiegevoelig gedrag in mutante P53 V157F borstkankercellen met behulp van multipoint Thioflavine T-fluorescentie (Video) | JoVE Journal

Detectie van aggregatiegevoelig gedrag in mutante P53 V157F borstkankercellen met behulp van multipoint Thioflavine T-fluorescentie

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