Name: Nieuw chirurgisch knaagdiermodel voor het bestuderen van opties voor de behandeling van neuroompijn met behulp van gerichte spierreïnnervatie door de nervus saphenos (Video) | JoVE Journal
Uploaded: 2025-11-14T13:55:00.000+00:00
Duration: 7 min
Description: Leer een reproduceerbaar rattenmodel voor neuroompijnonderzoek met behulp van microchirurgie en zenuwstimulatie. Dit protocol verbetert de translationele relevantie van preklinisch onderzoek naar klinische toepassingen.

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</ol>

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nieuw chirurgisch knaagdiermodel voor het bestuderen van opties voor de behandeling van neuroompijn met behulp van gerichte spierreïnnervatie door de nervus saphenos

Novel Surgical Rodent Model for Studying Neuroma Pain Treatment Options using Targeted Muscle Reinnervation Through the Saphenous Nerve

Nieuw chirurgisch knaagdiermodel voor het bestuderen van opties voor de behandeling van neuroompijn met behulp van gerichte spierreïnnervatie door de nervus saphenos

This protocol describes a novel surgical model to study nerve pain and surgical treatment in rats. Traditional neuroma models use mixed nerves, although ...

novel-surgical-rodent-model-for-studying-neuroma-pain-treatment

Research

JoVE Journal

Neuroscience

Neurowetenschap

De Gespaard zenuwletsel diermodel is hier beschreven als een muis model van perifere neuropathische pijn na een gedeeltelijke denervatie van de nervus ischiadicus door lesioning het scheenbeen en de gemeenschappelijke peroneus takken, waardoor de resterende Sural zenuw intact. Gedragsverandering als gevolg van mechanische allodynie wordt gekwantificeerd door von Frey filamenten.

De Gespaard Nerve Injury (SNI) Model van Induced mechanische allodynie in Muizen

Source: Brakkee, E. M., et al. Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats. J. Vis. Exp. (2023).This video demonstrates a procedure to generate a rat tibial neuroma transposition (TNT) model. After exposing the tibial nerve in the hind leg of an anesthetized rat, transect the nerve. Next, create a subcutaneous tunnel, thread the severed nerve through the tunnel, and suture it in place. In the absence of a target for reinnervation, disorganized axonal...

Generating a Rat Model of Tibial Neuroma Transposition

Neuropathische pijn is het gevolg van een letsel of ziekte die het somatosensorische systeem. De "cuff model" van neuropathische pijn bij muizen bestaat uit de inplanting van een polyethyleen manchet rond de belangrijkste tak van de nervus ischiadicus. Mechanische allodynie wordt getest met behulp van von Frey filamenten.

De heupzenuw handboeien Model van neuropathische pijn in Muizen

De aangepaste chirurgie is een vereenvoudigde methode voor een model voor gespaard zenuwletsel bij muizen of ratten dat slechts één ligatie en één snede vereist om zowel de gemeenschappelijke peroneale als de surale zenuwen te verwonden.

Gemodificeerd model voor gespaard zenuwletselchirurgie van neuropathische pijn bij muizen

Partiële heupzenuwligatie induceert langdurige chronische neuropathische pijn, gekenmerkt door overdreven reacties op thermische en mechanische stimuli. Dit muismodel van neuropathische pijn wordt vaak gebruikt om innovatieve therapieën voor pijnbestrijding te bestuderen. Dit artikel beschrijft in detail de chirurgische procedure om de standaardisatie en reproduceerbaarheid te verbeteren.

Partiële heupzenuwligatie: een muismodel van chronische neuropathische pijn om het antinociceptieve effect van nieuwe therapieën te bestuderen

Dit protocol beschrijft het tibiale neuroomtranspositiemodel, dat een laesie van de tibiale zenuw inhoudt met daaropvolgende transpositie van het proximale zenuwuiteinde naar een subcutane pretibiale of laterale positie. Gedragstesten van neuroompijn en plantaire hyperalgesie worden gekwantificeerd met behulp van Von Frey monofilamenten.

Chirurgie en gedragstesten in het tibiale neuroomtranspositiemodel bij ratten

Hier beschrijven we de chirurgische procedure om regeneratieve perifere zenuwinterface (RPNI)-chirurgie uit te voeren voor de behandeling van neuropathische pijn na amputatie in de context van een internationale, gerandomiseerde gecontroleerde studie (RCT) (ClinicalTrials.gov, NCT05009394). De RCT vergelijkt RPNI met twee andere chirurgische technieken, namelijk gerichte spierreïnnervatie (TMR) en neuroomexcisie in combinatie met intramusculaire transpositie.

Regeneratieve perifere zenuwinterface: chirurgisch protocol voor een gerandomiseerde gecontroleerde studie bij pijn na amputatie

Het protocol schetst de chirurgische procedure voor de behandeling van pijn na amputatie met behulp van gerichte spierreïnnervatie (TMR). TMR zal worden vergeleken met twee andere chirurgische technieken, met name regeneratieve perifere zenuwinterface (RPNI) en excisie van neuroom, gevolgd door onmiddellijke begraving in de spier in de context van een internationale, gerandomiseerde gecontroleerde studie.

Gerichte spierreïnnervatie: chirurgisch protocol voor een gerandomiseerde gecontroleerde studie bij pijn na amputatie

Dit protocol toont aan hoe een onderknie-amputatie bij ratten kan worden uitgevoerd en direct gerichte spierrenervatie (iTMR) kan worden uitgevoerd om strategieën te onderzoeken om amputatiegerelateerde pijn te behandelen en te voorkomen en om de pijnmechanismen die met amputatie samenhangen beter te begrijpen.

Rat achterpootamputatiemodel en directe gerichte spierrenervatie

Met behulp van functionele MRI en gedragsmatige methoden om de neurale representatie van de persoonlijke waarde van risicovolle en dubbelzinnige opties in het menselijk brein te bepalen.

Het meten van de subjectieve waarde van Risky en ambigue Opties met behulp van experimentele economie en functionele MRI methoden

Leer een reproduceerbaar rattenmodel voor neuroompijnonderzoek met behulp van microchirurgie en zenuwstimulatie. Dit protocol verbetert de translationele relevantie van preklinisch onderzoek naar klinische toepassingen.

Nieuw chirurgisch knaagdiermodel voor het bestuderen van opties voor de behandeling van neuroompijn met behulp van gerichte spierreïnnervatie door de nervus saphenos (Video) | JoVE Journal

Nieuw chirurgisch knaagdiermodel voor het bestuderen van opties voor de behandeling van neuroompijn met behulp van gerichte spierreïnnervatie door de nervus saphenos

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