Name: Formulering van vitamine A-tracerdoses voor toediening aan menselijke proefpersonen met behulp van de retinolisotoopverdunningstechniek (Video) | JoVE Journal
Uploaded: 2026-03-31T12:35:00.000+00:00
Duration: 8 min 45 s
Description: Leer hoe je vitamine A-isotooptracers bereidt voor menselijke studies met de retinolisotoopverdunningstechniek. Dit protocol beschrijft oplossen in plantaardige olie, kwaliteitscontrole en veilige toediening.

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<table><tbody><tr><td>2ml barnstenen glazen injectieflacons voor het verdelen van doses</td><td>Fisher</td><td>11573690</td><td>Afhankelijk van de monsterschaal van het onderzoek en het aantal vrijwilligers dat dagelijks wordt gedoseerd, kan het efficiënter zijn om grotere, bijv. 4ml, barnstenen injectieflacons te gebruiken</td></tr><tr><td>100ml volumetrische kolven</td><td>Fisher</td><td>11323644</td><td>Pyrex Borosilicaat Glas Klasse A Gecertificeerde Volumetrische Kolf</td></tr><tr><td>50ml volumetrische kolven</td><td>Fisher</td><td>11313644</td><td>Pyrex Borosilicaat Glas Klasse A Gecertificeerde Volumetrische Kolf</td></tr><tr><td>Analytische weegschalen&nbsp;</td><td>Fisher</td><td>13592970</td><td></td></tr><tr><td>Capillaire plunjerpompen (pipettips) CP100 10x96 TIPACK</td><td>Gilson</td><td>FD148314</td><td></td></tr><tr><td>Capillaire plunjerpompen CP1000ST 2x91</td><td>Gilson</td><td>F148180</td><td></td></tr><tr><td>Capillaire plunjerpompen CP250ST 6x96</td><td>Gilson</td><td>F148714</td><td></td></tr><tr><td>Karton cryobox</td><td>Starlab</td><td>A9623-8181</td><td></td></tr><tr><td>Hellma cuvet UV kwarts</td><td>SLS</td><td>CEL1600</td><td></td></tr><tr><td>Hexaan 95% n-Hexaan voor HPLC Gecertificeerd HPLC</td><td>Fisher</td><td>H/0406/17</td><td></td></tr><tr><td>Decklidjes voor 2ml barnstenen glazen injectieflacons</td><td>Fisher</td><td>11573542</td><td></td></tr><tr><td>Microman E M1000E Pipet (positieve verplaatsing)</td><td>Gilson</td><td>FD10006</td><td></td></tr><tr><td>Microman E M100E Pipet (positieve verplaatsing)</td><td>Gilson</td><td>FD10004</td><td></td></tr><tr><td>Microman E M250E Pipet (positieve verplaatsing)</td><td>Gilson</td><td>FD10005</td><td></td></tr><tr><td>Microscoop</td><td>Nikon</td><td>Eclipse E400</td><td></td></tr><tr><td>Microscoop glazen slides&nbsp;</td><td>Sigma</td><td>41122601</td><td></td></tr><tr><td>Sonicerende waterbad</td><td>VWR</td><td>USC100T</td><td></td></tr><tr><td>Spectrofotometer</td><td>Thermo Fisher Scientific</td><td>A51119600C</td><td></td></tr><tr><td>Stabiel isotoop gelabelde retinylacetaat tracer</td><td>Buchem BV</td><td>N/A</td><td>Een reeks stabiele isotopen kan worden gebruikt voor de RID-methode. Een lijst van verschillende commercieel verkrijgbare isotopen wordt gegeven in Tabel 1.&nbsp;</td></tr><tr><td>Ongeroerde waterbad</td><td>SLS</td><td>SLS1176</td><td></td></tr><tr><td>Plantaardige olie</td><td>Elke supermarkt</td><td>N/A</td><td>Het is belangrijk om verse plantaardige olie met voldoende houdbaarheid te gebruiken voor het bereiden van isotoopdoses.</td></tr><tr><td>Volac ongeplugde glazen pasteurpipet 150mm</td><td>SLS</td><td>PIP4150</td><td></td></tr></tbody></table>

formulering van vitamine a-tracerdoses voor toediening aan menselijke proefpersonen met behulp van de retinolisotoopverdunningstechniek

Formulation of Vitamin A Tracer Doses for Administration to Human Subjects using Retinol Isotope Dilution Technique

Formulering van vitamine A-tracerdoses voor toediening aan menselijke proefpersonen met behulp van de retinolisotoopverdunningstechniek

The retinol isotope dilution (RID) technique relies on the administration of isotopically labeled vitamin A tracers to human subjects. Here, we present a ...

formulation-vitamin-tracer-doses-for-administration-to-human-subjects

Research

JoVE Journal

Biochemistry

Biochemie

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Kwantitatieve analyse van vitamine A-metabolieten in oculaire en niet-oculaire weefsels van muizen met behulp van hoogwaardige vloeistofchromatografie

De adsorptie van peptiden op plastic tijdens traditionele seriële verdunningen met pipetpunten kan de bepaling van de potentie aanzienlijk beïnvloeden en het begrip van structuur-activiteitsrelaties verwarren die worden gebruikt voor fasen van loodidentificatie en loodoptimalisatie in de ontdekking van geneesmiddelen. Hier worden methoden beschreven voor geautomatiseerde akoestische non-contact seriële verdunning van peptidemonsters.

Geautomatiseerde Acoustic Afgifte voor de seriële verdunning van Peptide agonisten in potentie Bepaling Testen

Dit artikel beschrijft een methode voor het beoordelen van de interacties en assemblages van integrale membraaneiwitten in vitro met verschillende partnerfactoren in een lipid-proximaal milieu.

Gebruik Steiger Liposomen lipide-proximale eiwit-eiwit interacties reconstitueren In Vitro

Dit artikel beschrijft een methode voor het bestuderen van cellulair metabolisme in complexe gemeenschappen van meerdere celtypen, met behulp van een combinatie van stabiel isotooptracering, celsortering om specifieke celtypen te isoleren en massaspectrometrie.

Een methode voor het meten Metabolisme in gesorteerde subpopulaties van Complex Cell Gemeenschappen Met behulp van stabiele isotoop Tracing

Dit protocol beschrijft de reconstructie van nucleosomen die differentieel-isotoop gemerkte zus histonen. Tegelijkertijd asymmetrisch post-translationeel gemodificeerde nucleosomen worden gegenereerd na het gebruik van een histon premodified kopie. Deze preparaten kunnen gemakkelijk worden gebruikt om wijzigingen crosstalk mechanismen bestuderen, gelijktijdig op beide zus histonen, met behulp van hoge-resolutie NMR-spectroscopie.

Reconstructie van Nucleosomen met Differentieel Isotopen-gelabelde Sister Histon

Het menselijke netvlies is samengesteld uit functioneel en moleculair verschillende regio's, met inbegrip van de fovea, macula en perifere retina. Hier beschrijven we een methode met behulp van punch biopsieën en handmatig verwijderen van de lagen weefsel van een menselijk oog te ontleden en deze verschillende retinale regio's voor downstream Proteoom analyse te verzamelen.

Dissectie van menselijke netvlies en RPE-vaatvlies voor proteoom-analyse

Wij beschreven twee chemische-gebaseerde protocollen voor het kweken van muis embryonale stamcellen in detail. Deze nieuwe methode maakt gebruik van synergetische mechanismen ter bevordering van Tet-gemedieerde oxidatie (door vitamine C) en onderdrukken van DOVO synthese van 5-methylcytosine (door PD0325901) om DNA hypomethylation en pluripotent van muis embryonale stamcellen te onderhouden.

Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C

Hier presenteren we een protocol van geheel-cel elektrochemische experimenten te bestuderen van de bijdrage van proton vervoer aan het tarief van extracellulaire elektronentransport via de buitenmembraan cytochromes complex in Shewanella oneidensis heer-1.

Elektrochemische detectie van Deuterium kinetisch-isotoopeffect op extracellulaire elektronentransport in Shewanella oneidensis heer-1

Stabiele koolstof en zuurstof isotoop analyse van mens en dier tandglazuur carbonaat is gebruikt als een proxy voor afzonderlijke voeding en milieu wederopbouw. Hier, wij bieden een gedetailleerde beschrijving en visuele documentatie van bulk en sequentiële tooth glazuur bemonstering alsmede voorbehandeling van archeologisch en paleontologisch monsters.

Monstername en voorbehandeling van tandglazuur carbonaat voor stabiele koolstof en zuurstof isotoop analyse

Dit protocol beschrijft een kalibratie-vrije aanpak voor het kwantificeren van eiwit homo-oligomerisatie in vitro op basis van fluctuatiemarges Fluorescentiespectroscopie met behulp van commerciële licht scanning microscopie. De juiste overname-instellingen en de analysemethoden worden weergegeven.

Kalibratie-gratis In Vitro kwantificering van eiwit Homo-oligomerisatie met behulp van commerciële instrumentatie en Software van de analyse van de helderheid van gratis, Open Source

Leer hoe je vitamine A-isotooptracers bereidt voor menselijke studies met de retinolisotoopverdunningstechniek. Dit protocol beschrijft oplossen in plantaardige olie, kwaliteitscontrole en veilige toediening.

Formulering van vitamine A-tracerdoses voor toediening aan menselijke proefpersonen met behulp van de retinolisotoopverdunningstechniek (Video) | JoVE Journal

Formulering van vitamine A-tracerdoses voor toediening aan menselijke proefpersonen met behulp van de retinolisotoopverdunningstechniek

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