Name: Intravitale tweefotonbeeldvorming van aanrakingssensorische axonmorfologie in muizenhuid (Video) | JoVE Journal
Uploaded: 2025-12-30T13:50:00.000+00:00
Duration: 7 min 51 s
Description: Leer intravitale tweefotonbeeldvorming van aanrakingssensorische axonmorfologie in muizenhuid. Deze methode maakt hoogresolutiebeeldvorming van lage-drempelmechanoreceptoraxonterminals mogelijk voor onderzoek naar somatosensorische circuitontwikkeling.

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</ol>

<table><tbody><tr><td>20x Objective Lens &ndash; Super NA 1.0</td><td>Olympus</td><td>1-U2B965</td><td>Two-Photon Microscope Setup</td></tr><tr><td>22x60 Cover Slip</td><td>VWR</td><td>16004-330</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Air-Hardening Modeling Clay</td><td>DAS</td><td></td><td>Two-Photon Microscope Setup</td></tr><tr><td>Anesthesia Air Pump (HF), 110V</td><td>RWD Life Science Inc.</td><td>R510-30</td><td>Isoflurane System</td></tr><tr><td>Anesthesia Concentric Mask</td><td>RWD Life Science Inc.</td><td>R510-M</td><td>Isoflurane System</td></tr><tr><td>Anesthesia Induction Chamber &ndash; Mouse and Rat</td><td>RWD Life Science Inc.</td><td>V105</td><td>Isoflurane System</td></tr><tr><td>Bruker Ultima Investigator Plus Laser Scanning Microscope</td><td>Bruker Nano, Inc.&nbsp;</td><td></td><td>Two-Photon Microscope Setup</td></tr><tr><td>Butterfly Needles</td><td>Fisher Scientific</td><td>1484041</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Chameleon Discovery NX with TPC: Dual</td><td>Coherent</td><td>1385777</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Cone Mask with Tubing for Neonatal Mice</td><td>RWD Life Science Inc.</td><td>68680</td><td>Isoflurane System</td></tr><tr><td>Dual emission filters (et525/70m and et595/50m) and t565lpxr&nbsp; dichroic beam splitter</td><td>Nikon</td><td>INVP-01</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Epi-Fluorescence Filter Set ET-GFP ET470/40x and ET252/50m; dichroic T495LPXR&nbsp;</td><td>Nikon</td><td>518065</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Ethanol</td><td>Decon Laboratories Inc.</td><td></td><td>Two-Photon Microscope Setup</td></tr><tr><td>Far Infrared Warming Pad with Controller (AC power)</td><td>Kent Scientific</td><td>RT-0515</td><td>Two-Photon Microscope Setup</td></tr><tr><td>GaAsp Detectors (2)&nbsp;</td><td>Hamamatsu Photonics&nbsp;</td><td>HED-UP-2&nbsp;</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Gas Filter Canisters</td><td>RWD Life Science Inc.</td><td>R510-31-6</td><td>Isoflurane System</td></tr><tr><td>High Vacuum Grease</td><td>Sigma Aldrich</td><td>Z273554</td><td>Imaging System</td></tr><tr><td>Isoflurane, USP (250 mL)</td><td>Piramal Critical Care</td><td>6679-017-25</td><td>Isoflurane System</td></tr><tr><td>Laser Cover Box&nbsp;</td><td>Teco Technology</td><td></td><td>Imaging System</td></tr><tr><td>Mice: B6.129S6(Cg)-Ntrk2tm3.1(cre/ERT2)Ddg/J</td><td>JAX</td><td>27214</td><td></td></tr><tr><td>Mice: B6.Cg-Igs7tm140.1(tetO-EGFP,CAG-tTA2)Hze/J</td><td>JAX</td><td>30220</td><td></td></tr><tr><td>Mice: B6.Cg-Tg(Thy1-YFP)16Jrs/J</td><td>Charles River</td><td>3709</td><td></td></tr><tr><td>Nair Hair Remover Cream</td><td>Amazon</td><td>28001</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Raw Data Files</td><td>Zenodo</td><td></td><td>10.5281/zenodo.17370834</td></tr><tr><td>Rigid Stand with Large Rectangular Insert Holder</td><td>Thorlabs</td><td>MP15M</td><td>Two-Photon Microscope Setup</td></tr><tr><td>Small Animal Anesthesia Operation Platform</td><td>RWD Life Science Inc.</td><td>R510-PGL</td><td>Isoflurane System</td></tr><tr><td>Soothe Lubricant Eye Ointment</td><td>Bausch + Lomb</td><td></td><td>Two-Photon Microscope Setup</td></tr><tr><td>Superfrost Plus Microscope Slides</td><td>Fisher Scientific</td><td>12-550-15</td><td>Two-Photon Microscope Setup</td></tr></tbody></table>

intravitale tweefotonbeeldvorming van aanrakingssensorische axonmorfologie in muizenhuid

Intravital Two-Photon Imaging of Touch Sensory Axon Morphology in Mouse Skin

In dit werk werd tweefotonmicroscopie gebruikt om Aβ RA-LTMR en Aδ-LTMR axonale structuren in de huid tijdens de ontwikkeling en bij volwassenen te visualiseren. Volwassen lancetvormige uiteinden, die voorkomen bij muizen rond de postnatale dag 20 en ouder, zijn volledig ontwikkeld19,32. De algehele organisatie van axonterminalen in de harige huid van volwassen muizen werd afgebeeld, zoals weergegeven in de maximale intensiteitsprojectie (Fig...

Intravitale tweefotonbeeldvorming van aanrakingssensorische axonmorfologie in muizenhuid

Low-threshold mechanoreceptors (LTMRs) are somatosensory neurons that detect innocuous light touch stimuli such as vibration, hair deflection, and ...

intravital-two-photon-imaging-touch-sensory-axon-morphology-mouse

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Live-Imaging van de dorsale wortel Axonen na Rhizotomy

Source: Mizuno, H., et al. In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice. J. Vis. Exp. (2018)This video showcases two-photon microscopy being used to image neurons in the brain of an anesthetized transgenic mouse pup. The two-photon laser allows for deeper imaging with high spatial resolution and minimal light scattering, enabling the detailed capture of neuronal morphology, including dendritic projections.

Two-Photon Microscopy for Imaging Neuronal Morphology  in a Mouse Pup

Om de veranderingen van nociceptieve intraepidermal zenuwvezels (IENFs) in pijnlijke neuropathieën (PN) te bestuderen, ontwikkelden we protocollen die direct kon onderzoeken driedimensionale morfologische veranderingen waargenomen in nociceptieve IENFs. Drie-dimensionale analyse van IENFs heeft de potentie om de morfologische veranderingen van IENF in PN evalueren.

Driedimensionale beeldvorming van Nociceptieve Intraepidermal zenuwvezels in Menselijke huid Biopsieën

Het protocol voor dynamische longitudinale beeldvorming en selectieve laser laesie van zenuwuiteinden in reporter transgene muizen wordt gepresenteerd.

In vivo twee-foton microscopie van de Single zenuwuiteinden in de huid

Time-lapse beeldvorming in het levende dier levert waardevolle informatie over structurele reorganisatie in de intacte hersenen. Hier introduceren we een voorbereiding met een verdunde schedel die transcraniële beeldvorming mogelijk maakt van fluorescent gelabelde synaptische structuren in de levende muizencortex door middel van twee-foton microscopie.

Twee-Photon<em&gt; In vivo</em&gt; Imaging van vertakte stekels in de Mouse Cortex met een uitgedunde schedel Voorbereiding

Zoogdierhuid bevat een divers scala aan structuren - zoals haarfollikels en zenuwuiteinden - die onderscheidend patronen van ruimtelijke organisatie vertonen. Het analyseren van de huid als een platte berg maakt gebruik van de 2-dimensionale geometrie van dit weefsel tot volledige dikte van hoge-resolutie afbeeldingen van de huid structuren te produceren.

Flat Mount beeldvorming van de huid van muizen en de toepassing ervan op de Analyse van Haarzakje Patterning en Sensorische Axon morfologie

Twee-foton intravital beeldvorming kan worden gebruikt om interacties tussen verschillende celtypen in het ruggenmerg in hun oorspronkelijke weefselomgeving in een botmerg chimerisch dier met een traumatische ruggenmergverplettering in de dorsale kolom te onderzoeken.

Intravitale Imaging van Axonale Interacties met Microglia en macrofagen in een muis Dorsale Column Crush Injury

Deze video toont de craniotomieprocedure die chronische beeldvorming van neuronen in de retrospleniale cortex van muizen mogelijk maakt met in vivo twee-foton microscopie in de Thy1-GFP transgene lijn. Deze benadering wordt gecombineerd met injectie van mCherry-expresserend adeno-geassocieerd virus in de dorsale hippocampus. Deze technieken maken langdurige monitoring van ervaringsafhankelijke structurele plasticiteit in RSC mogelijk.

Simultane twee-foton<em&gt; In Vivo</em&gt; Beeldvorming van Synaptic In- en Postsynaptische Doelen op Mouse retrospleniale Cortex

We presenteren een geoptimaliseerde lokale ejectieprocedure met behulp van een glazen micro-pipet en een snelle twee-fotonen hyperstack-beeldvormings methode, die nauwkeurige meting van veranderingen in de capillaire diameter mogelijk maakt en het onderzoek van de verordening in drie dimensies.

In vivo drie-dimensionale twee-Fotonmicroscopie om uitgevoerde vasculaire responsen te bestuderen door lokale ATP-ejectie met behulp van een glazen micro pipet

Morfologische veranderingen optreden in immuun responsieve fibroblast cellen na activering en het bevorderen van veranderingen in cellulaire werving. Gebruik makend van 2-Photon Imaging in combinatie met een genetisch gemanipuleerde fibroblast-specifieke proteïne 1 (FSP1)-CRE; tdTomato floxed-stop-floxed (TB/TB) muis lijn en groene fluorescently gelabeld lipopolysaccharide-FITC, we kunnen zeer specifieke opname van lipopolysaccharide in dermale fibroblasten en morfologische veranderingen in...

In vivo tweekleurige 2-Fobe beeldvorming van genetisch gelabelde reporter cellen in de huid

Leer intravitale tweefotonbeeldvorming van aanrakingssensorische axonmorfologie in muizenhuid. Deze methode maakt hoogresolutiebeeldvorming van lage-drempelmechanoreceptoraxonterminals mogelijk voor onderzoek naar somatosensorische circuitontwikkeling.

Intravitale tweefotonbeeldvorming van aanrakingssensorische axonmorfologie in muizenhuid

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