Name: Hepatitis E Virus Fluorescentiereductie Neutralisatietest voor het meten van neutraliserende antilichaamtiters (Video) | JoVE Journal
Uploaded: 2026-05-22T13:30:00.000+00:00
Duration: 7 min 58 s
Description: Leer de fluorescentiereductie neutralisatietest (FRNT) voor het meten van neutraliserende antilichaamtiters tegen Hepatitis E-virus. Deze methode biedt een waardevol alternatief voor PRNT bij niet-cytopathische virussen.

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5 minutes

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<table><tbody><tr><td>1.5 mL microfuge tubes</td><td>Axygen</td><td>MCT-150-C</td><td></td></tr><tr><td>12-well cell culture dish</td><td>Corning</td><td>3512</td><td></td></tr><tr><td>37 degree incubator shaker</td><td>Thermo Scientific</td><td>50163013</td><td></td></tr><tr><td>4&prime;,6-diamidino-2-phenylindole</td><td>Abcam</td><td>AB104139</td><td>DAPI</td></tr><tr><td>50mL centrifuge tube</td><td>Falcon</td><td>352070</td><td></td></tr><tr><td>6-well cell culture dish</td><td>Corning&nbsp;</td><td>3516</td><td></td></tr><tr><td>Alexa flour 594 secondary IgG Antibody</td><td>Thermo Scientific</td><td>A-11012</td><td></td></tr><tr><td>Anti-ORF2 antibody</td><td>Genscript</td><td>NA</td><td>Op bestelling gesynthetiseerd door Genscript en gekenmerkt in ons laboratorium14</td></tr><tr><td>Anti-rabbit IgG antibody</td><td>Genscript</td><td>NA</td><td></td></tr><tr><td>Bovine Serum Albumin</td><td>HIMEDIA</td><td>MB083</td><td>BSA</td></tr><tr><td>Confocal microscope</td><td>Olympus</td><td>FV3000</td><td></td></tr><tr><td>DMEM, high glucose</td><td>HIMEDIA</td><td>AL007A</td><td></td></tr><tr><td>Fetal Bovine Serum</td><td>GIBCO</td><td>16000-044</td><td>FBS</td></tr><tr><td>ImageJ 1.54g</td><td>ImageJ.org (Wayne Rasband en medewerkers, NIH, USA)</td><td></td><td></td></tr><tr><td>Normal Goat Serum</td><td>HIMEDIA</td><td>RM-10701</td><td></td></tr><tr><td>Paraformaldehyde</td><td>HIMEDIA</td><td>GRN3660</td><td>PFA</td></tr><tr><td>Phosphate Buffer Saline pH 7.4</td><td>HIMEDIA</td><td>TL1006</td><td>PBS</td></tr><tr><td>Polyethyleneglycol 6000</td><td>SIGMA</td><td>81255</td><td>PEG6000</td></tr><tr><td>SOLIScript 1-step Probe Kit</td><td>SOLIS BIODYNE</td><td>08-57-00250</td><td></td></tr><tr><td>TRI reagent</td><td>Molecular Research Centre</td><td>TR118</td><td></td></tr><tr><td>Triton X100</td><td>SIGMA</td><td>X100</td><td></td></tr><tr><td>Trypsin-EDTA</td><td>GIBCO</td><td>25300-54</td><td></td></tr></tbody></table>

hepatitis e virus fluorescentiereductie neutralisatietest voor het meten van neutraliserende antilichaamtiters

Hepatitis E Virus Fluorescence Reduction Neutralization Test for Measuring Neutralizing Antibody Titers

Hepatitis E Virus Fluorescentiereductie Neutralisatietest voor het meten van neutraliserende antilichaamtiters

Virus neutralization assay measures the neutralization potential of an antibody or a mixture of antibodies against the corresponding virus. It is a ...

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Research

JoVE Journal

Immunology and Infection

Immunologie en Infectie

This video demonstrates Microneutralization assays to measure neutralizing antibodies using Madin-Darby Canine Kidney cells overexpressing α2,6-linked sialic acid in human sera. This assay is often used for influenza human...

A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum

This video demonstrates an assay measuring influenza neuraminidase (NA) inhibiting antibody titers in a serum sample. A serum sample containing antibodies against NA is added to a fetuin-coated microwell plate. Upon adding the virus, the antibodies prevent viral NA from cleaving fetuin and exposing its galactose moiety. A peroxidase-conjugated lectin is then added, which binds to the exposed galactose. The NA-inhibiting antibody titer is determined using a colorimetric test, where a peroxidase...

An Assay to Measure Influenza Neuraminidase Inhibition Antibody Titers

Source:Matthew J Murray1, Gary R McLean2,3, Matthew B Reeves11Institute of Immunity and Transplantation, University College London.2Cellular and Molecular Immunology Research Centre, London Metropolitan University.3National Heart and Lung Institute, Imperial College London.This video demonstrates an immunofluorescence assay designed to measure the neutralization of Human Cytomegalovirus (HCMV). Virus-specific antibodies are mixed with the virus and incubated with human fibroblast cells. The...

An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus

Source:Kelly Da Costa1, Diego Cantoni1,2, Martin Mayora-Neto1, Tobia Fantoni1,3, Nigel Temperton11Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent.2MRC-University of Glasgow Centre for Virus Research, University of Glasgow.3Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona.This video showcases a neutralization assay measuring serum efficacy in neutralizing SARS-CoV-2 viruses. It involves pseudotyped viruses, serum dilution, and...

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We beschrijven de enzym-gekoppelde lectin-assay (ELLA) voor het meten van influenza-neuraminidase (NA)-remmingsantilichaam-titers in sera. De assay gebruikt pinda-agglutinine om galactoseresiduen te kwantificeren die toegankelijk worden wanneer NA siaalzuur verwijdert van fetuïne-gecoate, 96-well platen.

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Hier beschreven is een effectieve methode voor het produceren van hoge virale titer voorraden van hepatitis E-virus (HEV) om efficiënt te infecteren hepatoma cellen. Met de gepresenteerde methode kunnen zowel niet-omhulde, als omhulde virale deeltjes worden geoogst en gebruikt voor het inenten van verschillende cellijnen.

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Leer de fluorescentiereductie neutralisatietest (FRNT) voor het meten van neutraliserende antilichaamtiters tegen Hepatitis E-virus. Deze methode biedt een waardevol alternatief voor PRNT bij niet-cytopathische virussen.

Hepatitis E Virus Fluorescentiereductie Neutralisatietest voor het meten van neutraliserende antilichaamtiters

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