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<table><tbody><tr><td>Benzalkonium Chloride&nbsp;</td><td>Merck, Spain</td><td>8.14363.0100</td><td>Biocide</td></tr><tr><td>Centrifuged Mega Star 600R</td><td>VWR, USA</td><td>VWR Mega Star 600R</td><td>-</td></tr><tr><td>Combenefit - Version 2.021</td><td>Cancer Research UK Cambridge Institute, Cambridge, United kingdom</td><td>-</td><td>Software voor synergiebeoordeling</td></tr><tr><td>Cuvettes</td><td>VWR, Germany</td><td>VWRI634-0678</td><td>1,5 mL cuvettes voor optische dichtheidsaanpassing</td></tr><tr><td>Destilatted water</td><td>-</td><td>-</td><td>Voor media-bereiding</td></tr><tr><td>Dimethyl Sulphoxide (DMSO)</td><td>VWR, France</td><td>23500.322</td><td>Organische oplosmiddel</td></tr><tr><td>Disposable inoculation loops</td><td>VWR, Italy</td><td>VWRI612-9357</td><td>10 μL inoculatielussen</td></tr><tr><td>Eppendorf tubes</td><td>VWR, China</td><td>VWRI525-1164</td><td>1,5 mL eppendorf safe-lock tubes&nbsp;</td></tr><tr><td>Escherichia coli</td><td>Spanish Type Culture Collection (CECT)</td><td>434</td><td>Referentiestam gebruikt in dit onderzoek</td></tr><tr><td>Ethanol</td><td>VWR, France</td><td>83813.36</td><td>Voor ontsmetting van werkplek, materiaal en handen</td></tr><tr><td>Eugenol 99%</td><td>Sigma-Aldrich, USA</td><td>163333</td><td>Fytochemisch</td></tr><tr><td>Falcon tubes</td><td>VWR, USA</td><td>VWRI525-1085</td><td>15 mL falcon tubes</td></tr><tr><td>Falcon tubes</td><td>VWR, Belgium</td><td>VWRI525-1109</td><td>50 mL falcon tubes</td></tr><tr><td>GraphPad Prism 9.0 for Windows 11</td><td>La Jolla, California, USA</td><td>-</td><td>Software voor wiskundige en statistische analyse</td></tr><tr><td>Lecithin</td><td>Alfa Aesar, Germany</td><td>Q04A010</td><td>-</td></tr><tr><td>L-histidine</td><td>Merck, USA</td><td>1.04351.0025</td><td>-</td></tr><tr><td>Orbital Incubator</td><td>VWR, USA</td><td>VWR 5000I Incubating shaker 230V</td><td>-</td></tr><tr><td>Peracetic acid 15%</td><td>AppliChem, Germany</td><td>143495-1211</td><td>Biocide</td></tr><tr><td>Petri dishes</td><td>VWR, Italy</td><td>VWRI391-0582</td><td>-</td></tr><tr><td>Pipette 1 - 10 mL</td><td>VWR, USA</td><td></td><td>-</td></tr><tr><td>Pipette 100 - 1000 μL</td><td>VWR, USA</td><td></td><td>-</td></tr><tr><td>Pipette tips 100 - 1000 μL</td><td>Frilabo, Portugal</td><td>1-202-50-0</td><td>-</td></tr><tr><td>Pippette 20 - 200 μL</td><td>VWR, USA</td><td></td><td>-</td></tr><tr><td>Pippette tips 1 - 10 mL</td><td>VWR, Belgium</td><td>vwri613-7094</td><td>-</td></tr><tr><td>Pippette tips 2 - 200 μL</td><td>VWR, Mexico</td><td>vwri613-6418</td><td>-</td></tr><tr><td>Plate Count Agar</td><td>VWR, Belgium</td><td>84608-0500</td><td>Isolatie en cultivatie van micro-organismen</td></tr><tr><td>Polysorbate 80</td><td>VWR, USA</td><td>20E2756726</td><td>-</td></tr><tr><td>Potassium Chloride - KCl&nbsp;</td><td>VWR, Belgium</td><td>11D110008</td><td>-</td></tr><tr><td>Potassium Dihydrogen Phosphate - KH2PO4</td><td>VWR, Germany</td><td>26931.263</td><td>-</td></tr><tr><td>Sacilicic acid</td><td>Sigma-Aldrich, USA</td><td>84210-500G</td><td>Fytochemisch</td></tr><tr><td>Saponin</td><td>VWR, Germany</td><td>21A124103</td><td>-</td></tr><tr><td>Sodium Chloride - NaCl</td><td>VWR, Belgium</td><td>27788.297</td><td>-</td></tr><tr><td>Sodium Hydrogen Phosphate - Na2HPO4</td><td>VWR, Belgium</td><td>28026.26</td><td>-</td></tr><tr><td>Sodium Thiosulphate - Na2S2O3</td><td>VWR, USA</td><td>18D2056589</td><td>-</td></tr><tr><td>Spectrophotometer</td><td>VWR, USA</td><td>UV-1600PC Spectrophotometer</td><td>Opticale dichtheidsaanpassing</td></tr><tr><td>Staphylococcus aureus</td><td>Spanish Type Culture Collection (CECT)</td><td>976</td><td>Referentiestam gebruikt in dit onderzoek</td></tr><tr><td>Tryptic Soy Broth&nbsp;</td><td>Merck, Germany</td><td>1.05459.0500</td><td>Isolatie en cultivatie van micro-organismen</td></tr><tr><td>Ultrapure water</td><td>-</td><td>-</td><td>Voor monstervoorbereiding</td></tr></tbody></table>

in vitro evaluatie van de versterkende effecten van plantaardige moleculen op conventionele biociden

In Vitro Evaluation of the Potentiating Effects of Plant-Derived Molecules on Conventional Biocides

In vitro Evaluatie van de versterkende effecten van plantaardige moleculen op conventionele biociden

Developing innovative and efficient approaches to enhance the antimicrobial activity of conventional biocides is a promising strategy for improving ...

in-vitro-evaluation-potentiating-effects-plant-derived-molecules-on

Research

JoVE Journal

Biology

Biologie

University of Porto.  Dit protocol biedt een systematisch kader om te beoordelen hoe fytochemicaliën de bactericide effecten van traditionele biociden kunnen versterken met behulp van bactericide activiteitsmodellering en combinatorische interactie-assays, die gericht zijn op het identificeren van synergetische mengsels die de microbiële controle verbeteren en het totale biocidegebruik verminderen.

In vitro Evaluatie van de versterkende effecten van plantaardige moleculen op conventionele biociden

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