Name: Cervicale lymfebuis-gecoruleerde rat-model voor het beoordelen van lymfetransport vanuit het hoofd en de hersenen (Video) | JoVE Journal
Uploaded: 2026-03-10T13:10:00.000+00:00
Duration: 8 min 3 s
Description: Leer hoe u de diepe cervicale lymfeduct kunt canuleren bij ratten om lymfe afkomstig van het centrale zenuwstelsel direct te verzamelen en te analyseren. Deze methode ondersteunt onderzoek naar lymfatische klaring en ziekteprocessen.

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<li>Bachmann, S. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+effects+of+anaesthesia+on+the+contractility+of+lymphatic+vessels+in+vivo%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">Differential effects of anaesthesia on the contractility of lymphatic vessels in vivo.</a> J Physiol. 597 (11), 2841-2852 (2019).</li>
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</ol>

<table><tbody><tr><td>30 gauge needle</td><td>Yushou Health</td><td></td><td>Gebruikt voor het verbinden van het proximale uiteinde van canule (0503) met de heparine spuit</td></tr><tr><td>Anaesthesiedispenser met verdamper</td><td>YYUN Instrument Company</td><td></td><td></td></tr><tr><td>Blunt forceps</td><td>RWD Life Science</td><td>F22002-10&nbsp;</td><td></td></tr><tr><td>Wattenstaafjes</td><td>Qingdao Shultz Biotechnology</td><td>MQ-02</td><td>Wordt gebruikt om spierweefsel tijdelijk op zijn plaats te houden</td></tr><tr><td>Verwarmde chirurgische pad</td><td>Shanghai Yiheng Instruments</td><td></td><td>Afmeting 15 &times; 20 cm</td></tr><tr><td>Heparine natrium</td><td>Shanghai Aladdin Biochemical Technology</td><td>H123383</td><td>Verdunning vereist voor gebruik</td></tr><tr><td>Insulien spuit (1 mL)</td><td>Yushou Health</td><td>U100-1ML</td><td>Alleen gebruikt voor heparine pre-coating</td></tr><tr><td>Isofluraanoplossing voor inhalatie</td><td>RWD Life Science</td><td>R510-22-10</td><td>Gebruikt voor anesthesie via verdamper</td></tr><tr><td>Markeerstiften</td><td>Elk merk</td><td>N/A</td><td></td></tr><tr><td>Micro-centrifugebuis (1,5 mL)</td><td>Nantong Hairui Experimental Equipment</td><td>HR10003</td><td>Gebruikt voor het verzamelen van lymfe</td></tr><tr><td>Micro-schaar</td><td>RWD Life Science</td><td>GD234BA0007925</td><td></td></tr><tr><td>Nylon chirurgische hechting&nbsp;</td><td>Shanghai Yiheng Instruments</td><td>4-0 35CM</td><td></td></tr><tr><td>Pipet (volume instelbaar, meetbereik 5 tot 50 &mu;L)</td><td>Dragon Laboratory Instruments Limited</td><td>7030301006</td><td>Gebruikt alleen voor het meten van weefselkleefstof</td></tr><tr><td>Pipet tips&nbsp;</td><td>Biosharp Life Sciences</td><td>BS-10-T</td><td>Gebruikt alleen voor het overbrengen van weefselkleefstof, geen specifiek vereist volume</td></tr><tr><td>Polyethyleen (PE) canule 0,5 mm OD, 0,3 mm ID (0503)</td><td>AniLab Scientific Instruments(Ningbo)</td><td>PE-0503</td><td></td></tr><tr><td>Polyethyleen (PE) canule 0,6 mm OD, 0,5 mm ID (0605)</td><td>AniLab Scientific Instruments(Ningbo)</td><td>PE-0605</td><td></td></tr><tr><td>Veiligheidsschaafmesjes</td><td>Gillette Shanghai</td><td>Q31/0115000301C004</td><td>Gebruikt alleen voor het scheren van vacht</td></tr><tr><td>Elastiek</td><td>Elk merk</td><td>N/A</td><td>Gebruikt voor het vasthouden van de voorpoten van ratten</td></tr><tr><td>Liniaal</td><td>Elk merk</td><td>N/A</td><td></td></tr><tr><td>Scapel mesjes (#23)</td><td>RWD Life Science</td><td>S31010-01</td><td></td></tr><tr><td>Natriumchloride (0,9%) oplossing voor infusie</td><td>Shijiazhuang No. 4 Pharmaceutical</td><td>H20066533 (goedkeuringsnummer)</td><td>Gebruikt alleen voor het spoelen van vacht</td></tr><tr><td>Sprague-Dawley ratten</td><td>Beijing Vital River Laboratory Animal Technology</td><td>101</td><td>Gewicht 220 tot 250 gram, geen leeftijdsvereiste</td></tr><tr><td>Chirurgische lamp</td><td>Beijing Heo Bio-Tech</td><td>XHA011-1</td><td></td></tr><tr><td>Chirurgische microscoop</td><td>Shanghai Cewei Optoelectronic Technology</td><td>SMZ755T</td><td>Dissectiemicroscoop zonder mechanische standaard, kan worden uitgerust met een intrekbare standaard</td></tr><tr><td>Weefselkleefstof</td><td>3M Vetbond</td><td>1469SB</td><td>Hoofdbestanddeel cyanoacrylaat&nbsp;</td></tr><tr><td>Weefselexpander (Colibri)</td><td>RWD Life Science</td><td>R22029-04</td><td>Uitbreidingsbereik 20mm/4cm</td></tr></tbody></table>

cervicale lymfebuis-gecoruleerde rat-model voor het beoordelen van lymfetransport vanuit het hoofd en de hersenen

Cervical Lymph Duct-Cannulated Rat Model for Assessing Lymphatic Transport from the Head and Brain

Cervicale lymfebuis-gecoruleerde rat-model voor het beoordelen van lymfetransport vanuit het hoofd en de hersenen

Lymph flows from the central nervous system (CNS) via a series of lymphatic vessels (LVs) and lymph nodes that converge at the cervical lymph ducts. In ...

cervical-lymph-duct-cannulated-rat-model-for-assessing-lymphatic

Research

JoVE Journal

Immunology and Infection

Immunologie en Infectie

This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.

Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid

This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.

Intraduodenal Cannulation in a Rat Model for Immunopathology Studies

In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.

Cannulation of the Carotid Artery in a Rat Model

Hier beschrijven we een techniek om de mesenteriale lymfevat te canuleren bij ratten, wat de kwantificering van lipiden- en geneesmiddeltransport via het lymfestelsel mogelijk maakt na intestinale toediening. De techniek kan worden aangepast om mesenteriale lymfeconcentraties en/of transport van vocht, immuuncellen, peptiden, eiwitten en lipofiele moleculen te beoordelen.

De Mesenteriale Lymfe Duct gecanuleerde Rat Model: Toepassing op de beoordeling van Intestinal Lymfatische Drug Transport

Hier beschrijven we een protocol voor het uitvoeren van de cisterna magna cannulation (CMc), een minimaal invasieve manier om verklikstoffen, substraten en signalering moleculen in de cerebrospinale vloeistof (CSF). In combinatie met de verschillende beeldvormende modaliteiten, kan CMc glymphatic systeem en CSF dynamics beoordeling, zowel als hersenen bestrijkende levering van verschillende verbindingen.

Canule implantatie in de Cisterna Magna van knaagdieren

Hier beschrijven we een nauwkeurige methode voor het verzamelen van thoracale kanaallymfocyten en het observeren van de migratie van darm-tropenlymfocyten in de patches van rat Peyer gedurende 3 uur met behulp van time-lapse fotografie. Deze techniek kan verduidelijken hoe de dynamiek van lymfocyten wordt beïnvloed onder inflammatoire omstandigheden.

Microscopische observatie van lymfocytendynamiek in rat Peyer's patches

Hier presenteren we een protocol om de stappen voor het anastomoseren van de diepe cervicale lymfeklier of het afferente lymfevaat met de achterste gezichtsader bij de rat duidelijk te definiëren, om de invloed van andere variabelen te minimaliseren en de uitvoering van chirurgisch onderzoek te standaardiseren.

Chirurgische protocollen voor diepe cervicale lymfoveneuze anastomose in een rattenmodel: lymfeklier- en lymfevatenanastomosen

Dit experimentele protocol beschrijft een leverlymfebuis-gecanuleerd rattenmodel dat directe en kwantitatieve beoordeling van leverlymfatische en vaatdrainage mogelijk maakt. Het model maakt gelijktijdige bemonstering van leverlymfe en systemisch bloed mogelijk om levertransport, metabolisme en immuunsignalering te onderzoeken, ter ondersteuning van studies naar geneesmiddelfarmacokinetiek en leverspecifieke biologie.

Een uitgebreid rattenmodel voor het beoordelen van levertransportroutes door gelijktijdige lymfatische en bloedvaatbemonstering

Een techniek voor het uitvoeren van intravitale microscopie van de lies lymfeklier (LN) is geschetst. Een dergelijke techniek maakt het mogelijk voor...

Intravitale Microscopie van de lies lymfkliertest

Recente ontwikkelingen in de 2-foton microscopie hebben real-time...

Intravitale Imaging van de muis knieholte lymfkliertest

Leer hoe u de diepe cervicale lymfeduct kunt canuleren bij ratten om lymfe afkomstig van het centrale zenuwstelsel direct te verzamelen en te analyseren. Deze methode ondersteunt onderzoek naar lymfatische klaring en ziekteprocessen.

Cervicale lymfebuis-gecoruleerde rat-model voor het beoordelen van lymfetransport vanuit het hoofd en de hersenen

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