Name: Isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten (Video) | JoVE Journal
Uploaded: 2026-04-03T13:08:00.000+00:00
Duration: 9 min 9 s
Description: Leer een protocol voor het isoleren van enkele kernen uit coronaire endarterectomieweefsel van CABG-patiënten. Deze methode behoudt nucleaire integriteit en ondersteunt transcriptoomanalyse van instabiele atherosclerotische plaques.

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5 minutes

<ol><li>Roth, G. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Global+burden+of+cardiovascular+diseases+and+risk+factors-+1990-2019%5BTitle%5D)+AND+%22J.+Am.+Coll.+Cardiol%22%5BJournal%5D)">Global burden of cardiovascular diseases and risk factors- 1990-2019.</a> J. Am. Coll. Cardiol. 76 (25), 2982-3021 (2020).</li>
<li>de Winther, M. P. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Translational+opportunities+of+single-cell+biology+in+atherosclerosis%5BTitle%5D)+AND+%22Eur.+Heart+J%22%5BJournal%5D)">Translational opportunities of single-cell biology in atherosclerosis.</a> Eur. Heart J. 44 (14), 1216-1230 (2023).</li>
<li>Mohebbati, R., Momeni-Moghaddam, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+role+of+red+blood+cells+in+cholesterol+accumulation+and+atherosclerotic+plaque+instability%3A+a+perspective+on+atherosclerosis%5BTitle%5D)+AND+%22Curr.+Cardiol.+Rev%22%5BJournal%5D)">The role of red blood cells in cholesterol accumulation and atherosclerotic plaque instability: a perspective on atherosclerosis.</a> Curr. Cardiol. Rev. 21 (5), (2025).</li>
<li>Ajoolabady, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inflammation+in+atherosclerosis%3A+pathophysiology+and+mechanisms%5BTitle%5D)+AND+%22Cell+Death+Dis%22%5BJournal%5D)">Inflammation in atherosclerosis: pathophysiology and mechanisms.</a> Cell Death Dis. 15 (11), 817(2024).</li>
<li>Dawson, L. P., Layland, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-risk+coronary+plaque+features%3A+a+narrative+review%5BTitle%5D)+AND+%22Cardiol.+Ther%22%5BJournal%5D)">High-risk coronary plaque features: a narrative review.</a> Cardiol. Ther. 11 (3), 319-335 (2022).</li>
<li>Tong, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Highly+sensitive+magnetic+particle+imaging+of+vulnerable+atherosclerotic+plaque+with+active+myeloperoxidase-targeted+nanoparticles%5BTitle%5D)+AND+%22Theranostics%22%5BJournal%5D)">Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque with active myeloperoxidase-targeted nanoparticles.</a> Theranostics. 12 (17), 7641-7641 (2022).</li>
<li>Lu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronary+computed+tomography+angiography+assessment+of+high-risk+plaques+in+predicting+acute+coronary+syndrome%5BTitle%5D)+AND+%22Front.+Cardiovasc.+Med%22%5BJournal%5D)">Coronary computed tomography angiography assessment of high-risk plaques in predicting acute coronary syndrome.</a> Front. Cardiovasc. Med. 8, (2021).</li>
<li>Kinoshita, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-risk+plaques+on+coronary+computed+tomography+angiography%5BTitle%5D)+AND+%22JACC+Cardiovasc.+Imaging%22%5BJournal%5D)">High-risk plaques on coronary computed tomography angiography.</a> JACC Cardiovasc. Imaging. 17 (4), 382-391 (2024).</li>
<li>Canu, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-invasive+multimodality+imaging+of+coronary+vulnerable+patient%5BTitle%5D)+AND+%22Front.+Cardiovasc.+Med%22%5BJournal%5D)">Non-invasive multimodality imaging of coronary vulnerable patient.</a> Front. Cardiovasc. Med. 9, (2022).</li>
<li>Liu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+coronary+computed+tomography+angiography-derived+plaque+features+in+the+diagnosis+of+optical+coherence+tomography-defined+vulnerable+plaques%5BTitle%5D)+AND+%22Quant.+Imaging+Med.+Surg%22%5BJournal%5D)">Assessment of coronary computed tomography angiography-derived plaque features in the diagnosis of optical coherence tomography-defined vulnerable plaques.</a> Quant. Imaging Med. Surg. 15 (3), 2029-2041 (2025).</li>
<li>Chiorescu, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vulnerable+atherosclerotic+plaque%3A+is+there+a+molecular+signature%5BTitle%5D)+AND+%22Int.+J.+Mol.+Sci%22%5BJournal%5D)">Vulnerable atherosclerotic plaque: is there a molecular signature.</a> Int. J. Mol. Sci. 23 (21), 13638(2022).</li>
<li>The applications of single-cell RNA sequencing in atherosclerotic disease. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Front.+Cardiovasc.+Med%22">Front. Cardiovasc. Med</a>. Slenders, L., Tessels, D. E., van der Laan, S. W., Pasterkamp, G., Mokry, M. 9, (2022).</li>
<li>Adam, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Novel+biomarkers+of+atherosclerotic+vascular+disease-latest+insights+in+the+research+field%5BTitle%5D)+AND+%22Int.+J.+Mol.+Sci%22%5BJournal%5D)">Novel biomarkers of atherosclerotic vascular disease-latest insights in the research field.</a> Int. J. Mol. Sci. 23 (9), 4998(2022).</li>
<li>Ye, W., Chen, Z., Xia, Z., Li, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+RNA+sequencing+in+human+atherosclerotic+plaques+reveals+a+novel+smooth+muscle+cell+subtype+that+possesses+multi+differentiation+potential+and+shapes+the+microenvironment%5BTitle%5D)+AND+%22Clin.+Exp.+Med%22%5BJournal%5D)">Single-cell RNA sequencing in human atherosclerotic plaques reveals a novel smooth muscle cell subtype that possesses multi differentiation potential and shapes the microenvironment.</a> Clin. Exp. Med. 25 (1), 251(2025).</li>
<li>Kelly, J. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronary+endarterectomy%3A+analysis+of+the+society+of+thoracic+surgeons+adult+cardiac+surgery+database%5BTitle%5D)+AND+%22Ann.+Thorac.+Surg%22%5BJournal%5D)">Coronary endarterectomy: analysis of the society of thoracic surgeons adult cardiac surgery database.</a> Ann. Thorac. Surg. 114 (3), 667-674 (2022).</li>
<li>Ghatanatti, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronary+endarterectomy%3A+recent+trends%5BTitle%5D)+AND+%22J.+Clin.+DIAGNOSTIC+Res%22%5BJournal%5D)">Coronary endarterectomy: recent trends.</a> J. Clin. DIAGNOSTIC Res. , (2017).</li>
<li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+transcriptome+analysis+reveals+dynamic+cell+populations+and+differential+gene+expression+patterns+in+control+and+aneurysmal+human+aortic+tissue%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Single-cell transcriptome analysis reveals dynamic cell populations and differential gene expression patterns in control and aneurysmal human aortic tissue.</a> Circulation. 142 (14), 1374-1388 (2020).</li>
<li>Alsaigh, T., Evans, D., Frankel, D., Torkamani, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Decoding+the+transcriptome+of+calcified+atherosclerotic+plaque+at+single-cell+resolution%5BTitle%5D)+AND+%22Commun.+Biol%22%5BJournal%5D)">Decoding the transcriptome of calcified atherosclerotic plaque at single-cell resolution.</a> Commun. Biol. 5 (1), 1084(2022).</li>
<li>Iqbal, F., Lupieri, A., Aikawa, M., Aikawa, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Harnessing+single-cell+RNA+sequencing+to+better+understand+how+diseased+cells+behave+the+way+they+do+in+cardiovascular+disease%5BTitle%5D)+AND+%22Arterioscler.+Thromb.+Vasc.+Biol%22%5BJournal%5D)">Harnessing single-cell RNA sequencing to better understand how diseased cells behave the way they do in cardiovascular disease.</a> Arterioscler. Thromb. Vasc. Biol. 41 (2), 585-600 (2021).</li>
<li>Ahmad, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single+cell+RNA+sequencing+of+haematopoietic+cells+in+fresh+and+frozen+human+atheroma+tissue%5BTitle%5D)+AND+%22Cardiovasc.+Res%22%5BJournal%5D)">Single cell RNA sequencing of haematopoietic cells in fresh and frozen human atheroma tissue.</a> Cardiovasc. Res. 121 (3), 396-404 (2025).</li>
<li>Depuydt, M. A. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microanatomy+of+the+human+atherosclerotic+plaque+by+single-cell+transcriptomics%5BTitle%5D)+AND+%22Circ.+Res%22%5BJournal%5D)">Microanatomy of the human atherosclerotic plaque by single-cell transcriptomics.</a> Circ. Res. 127 (11), 1437-1455 (2020).</li>
<li>Williams, J. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single+cell+RNA+sequencing+in+atherosclerosis+research%5BTitle%5D)+AND+%22Circ.+Res%22%5BJournal%5D)">Single cell RNA sequencing in atherosclerosis research.</a> Circ. Res. 126 (9), 1112-1126 (2020).</li>
<li>Kim, N., Kang, H., Jo, A., Yoo, S. -A., Lee, H. -O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perspectives+on+single-nucleus+RNA+sequencing+in+different+cell+types+and+tissues%5BTitle%5D)+AND+%22J.+Pathol.+Transl.+Med%22%5BJournal%5D)">Perspectives on single-nucleus RNA sequencing in different cell types and tissues.</a> J. Pathol. Transl. Med. 57 (1), 52-59 (2023).</li>
<li>Gruenbaum, Y., Foisner, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lamins%3A+Nuclear+intermediate+filament+proteins+with+fundamental+functions+in+nuclear+mechanics+and+genome+regulation%5BTitle%5D)+AND+%22Annu.+Rev.+Biochem%22%5BJournal%5D)">Lamins: Nuclear intermediate filament proteins with fundamental functions in nuclear mechanics and genome regulation.</a> Annu. Rev. Biochem. 84 (1), 131-164 (2015).</li>
<li>Zwerger, M., Ho, C. Y., Lammerding, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nuclear+mechanics+in+disease%5BTitle%5D)+AND+%22Annu.+Rev.+Biomed.+Eng%22%5BJournal%5D)">Nuclear mechanics in disease.</a> Annu. Rev. Biomed. Eng. 13 (1), 397-428 (2011).</li>
<li>Dechat, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nuclear+lamins%3A+major+factors+in+the+structural+organization+and+function+of+the+nucleus+and+chromatin%5BTitle%5D)+AND+%22Genes+Dev%22%5BJournal%5D)">Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin.</a> Genes Dev. 22 (7), 832-853 (2008).</li>
<li>Oh, J. -M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+cell+type+distribution+between+single-cell+and+single-nucleus+RNA+sequencing%3A+enrichment+of+adherent+cell+types+in+single-nucleus+RNA+sequencing%5BTitle%5D)+AND+%22Exp.+Mol.+Med%22%5BJournal%5D)">Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing.</a> Exp. Mol. Med. 54 (12), 2128-2134 (2022).</li>
<li>Nott, A., Schlachetzki, J. C. M., Fixsen, B. R., Glass, C. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nuclei+isolation+of+multiple+brain+cell+types+for+omics+interrogation%5BTitle%5D)+AND+%22Nat.+Protoc%22%5BJournal%5D)">Nuclei isolation of multiple brain cell types for omics interrogation.</a> Nat. Protoc. 16 (3), 1629-1646 (2021).</li>
<li>Gulko, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protocol+for+flow+cytometry-assisted+single-nucleus+RNA+sequencing+of+human+and+mouse+adipose+tissue+with+sample+multiplexing%5BTitle%5D)+AND+%22STAR+Protoc%22%5BJournal%5D)">Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing.</a> STAR Protoc. 5 (1), 102893(2024).</li>
<li>Fernandez, D. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+immune+landscape+of+human+atherosclerotic+plaques%5BTitle%5D)+AND+%22Nat.+Med%22%5BJournal%5D)">Single-cell immune landscape of human atherosclerotic plaques.</a> Nat. Med. 25 (10), 1576-1588 (2019).</li>
<li>Galvis, A. E., Fisher, H., Camerini, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((NP-40+fractionation+and+nucleic+acid+extraction+in+mammalian+cells%5BTitle%5D)+AND+%22BIO-PROTOCOL%22%5BJournal%5D)">NP-40 fractionation and nucleic acid extraction in mammalian cells.</a> BIO-PROTOCOL. 7 (20), (2017).</li>
<li>Wirka, R. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Atheroprotective+roles+of+smooth+muscle+cell+phenotypic+modulation+and+the+TCF21+disease+gene+as+revealed+by+single-cell+analysis%5BTitle%5D)+AND+%22Nat.+Med%22%5BJournal%5D)">Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis.</a> Nat. Med. 25 (8), 1280-1289 (2019).</li>
<li>Pan, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+genomics+reveals+a+novel+cell+state+during+smooth+muscle+cell+phenotypic+switching+and+potential+therapeutic+targets+for+atherosclerosis+in+mouse+and+human%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Single-cell genomics reveals a novel cell state during smooth muscle cell phenotypic switching and potential therapeutic targets for atherosclerosis in mouse and human.</a> Circulation. 142 (21), 2060-2075 (2020).</li>
<li>Bakken, T. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-nucleus+and+single-cell+transcriptomes+compared+in+matched+cortical+cell+types%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Single-nucleus and single-cell transcriptomes compared in matched cortical cell types.</a> PLoS One. 13 (12), e0209648(2018).</li>
<li>Litviňuková, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cells+of+the+adult+human+heart%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Cells of the adult human heart.</a> Nature. 588 (7838), 466-472 (2020).</li>
<li>Hu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-nucleus+transcriptomic+survey+of+cell+diversity+and+functional+maturation+in+postnatal+mammalian+hearts%5BTitle%5D)+AND+%22Genes+Dev%22%5BJournal%5D)">Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts.</a> Genes Dev. 32 (19-20), 1344-1357 (2018).</li>
<li>Rani, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+use+of+RPMI+Medium+to+preserve+cell+morphology+for+pleural%2Fperitoneal+fluid+cytology%5BTitle%5D)+AND+%22J.+Cytol%22%5BJournal%5D)">Evaluation of use of RPMI Medium to preserve cell morphology for pleural/peritoneal fluid cytology.</a> J. Cytol. 39 (1), 26-29 (2022).</li>
<li>Machado, L., Relaix, F., Mourikis, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Stress+relief%3A+emerging+methods+to+mitigate+dissociation-induced+artefacts%5BTitle%5D)+AND+%22Trends+Cell+Biol%22%5BJournal%5D)">Stress relief: emerging methods to mitigate dissociation-induced artefacts.</a> Trends Cell Biol. 31 (11), 888-897 (2021).</li>
<li>Slyper, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+single-cell+and+single-nucleus+RNA-seq+toolbox+for+fresh+and+frozen+human+tumors%5BTitle%5D)+AND+%22Nat.+Med%22%5BJournal%5D)">A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors.</a> Nat. Med. 26 (5), 792-802 (2020).</li>
<li>Krishnaswami, S. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+single+nuclei+for+RNA-seq+to+capture+the+transcriptome+of+postmortem+neurons%5BTitle%5D)+AND+%22Nat.+Protoc%22%5BJournal%5D)">Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons.</a> Nat. Protoc. 11 (3), 499-524 (2016).</li>
</ol>

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<table><tbody><tr><td>100 &mu;m strainer</td><td>Miltenyi Biotech&nbsp;</td><td>130-098-463</td><td></td></tr><tr><td>30 &mu;m strainer</td><td>Miltenyi Biotech&nbsp;</td><td>130-098-458</td><td></td></tr><tr><td>Bovine Serum Albumin</td><td>HiMedia</td><td>TC546-25G</td><td></td></tr><tr><td>Centrifuge</td><td>MPW</td><td>NA</td><td>https://mpw.pl/en/offer/mpw-352r</td></tr><tr><td>Compound Microscope</td><td>Nikon</td><td>NA&nbsp;</td><td>Nikon Eclipse Ts2 inverted microscope (https://www.microscope.healthcare.nikon.com/en_AOM/products/inverted-microscopes/eclipse-ts2)</td></tr><tr><td>Confocal Microscope&nbsp;</td><td>Nikon</td><td>NA</td><td>Nikon Ti2-E inverted confocal microscope (https://www.microscope.healthcare.nikon.com/en_AOM/products/inverted-microscopes/eclipse-ti2-series)</td></tr><tr><td>Coverslips</td><td>HiMedia</td><td>BG011C</td><td></td></tr><tr><td>CRYOCHIL Wide Mouth Specimen Vial</td><td>Tarsons</td><td>883191</td><td></td></tr><tr><td>DAPI</td><td>Sigma</td><td>D9542-5MG</td><td></td></tr><tr><td>DPBS</td><td>Gibco (Thermofischer)</td><td>10010-023</td><td></td></tr><tr><td>Centrifuge Tube (15 mL)</td><td>Tarsons</td><td>546021</td><td></td></tr><tr><td>Flow cytometer&nbsp;</td><td>BD Biosciences</td><td>663029</td><td>BD FACSLyric (3L12C Instrument CEIVD)</td></tr><tr><td>Hemocytometer</td><td>Axibio</td><td>CCB 100</td><td></td></tr><tr><td>Microcentrifuge Tube (1.5 mL)</td><td>Tarsons</td><td>500010C</td><td></td></tr><tr><td>Microcentrifuge Tube (5 mL)</td><td>Tarsons</td><td>500050</td><td></td></tr><tr><td>MgCl2</td><td>Sigma</td><td>M1028-100ML</td><td></td></tr><tr><td>Micro pestle</td><td>Tarsons</td><td>160020</td><td></td></tr><tr><td>NaCl</td><td>Sigma</td><td>S9888-500G</td><td></td></tr><tr><td>NP-40</td><td>HiMedia</td><td>MB143-100ML</td><td></td></tr><tr><td>Nuclease free water</td><td>Gibco (Thermofischer)</td><td>W4502-1L</td><td></td></tr><tr><td>RNase inhibitor</td><td>Sigma</td><td>333540201</td><td></td></tr><tr><td>RPMI 1640</td><td>Gibco (Thermofischer)</td><td>11875093</td><td></td></tr><tr><td>Slide&nbsp;</td><td>Riviera</td><td>72910135</td><td></td></tr><tr><td>Tris-HCl</td><td>Sigma</td><td>T2194-100ML</td><td></td></tr><tr><td>Trypan blue&nbsp;</td><td>Sigma</td><td>302643-25G</td><td></td></tr></tbody></table>
```

isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten

Single Nuclei Isolation from Coronary Endarterectomy Tissue of Coronary Artery Bypass Graft Patients

Isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten

Atherosclerosis is a chronic, progressive inflammatory condition characterized by the accumulation of plaque in the walls of arteries. During the ...

single-nuclei-isolation-from-coronary-endarterectomy-tissue-coronary

Research

JoVE Journal

Biology

Biologie

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Isolatie van cardiomyocyten Kernen van Post-mortem weefsel

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Isolatie van Murine Valve endotheelcellen

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Isolatie van muis coronaire endotheelcellen

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We presenteren een protocol voor de isolatie van endocardiale en coronaire endotheelcellen uit rattenharten door sequentiële weefselvertering in een spijsverteringsbuffer, celverzameling uit terugkerende centrifugecycli en celzuivering met behulp van antiratten-CD31 microbeads.

Isolatie van endocardiale en coronaire endotheelcellen van de Ventriculaire Vrije Muur van het Hart van de Rat

Hier presenteren we een protocol dat de voorbereiding van celkernen beschrijft. Na microdissectie en enzymatische dissociatie van hartweefsel in afzonderlijke cellen, werden de voorlopercellen bevroren, gevolgd door isolatie van zuivere levensvatbare cellen, die werden gebruikt voor single-nucleus RNA-sequencing en de single-nucleus assay voor transposase-toegankelijk chromatine met high-throughput sequencing-analyses.

Kernisolatie van hartvoorlopercellen van muizen voor epigenoom- en genexpressieprofilering met eencellige resolutie

Deze studie presenteert een chirurgisch varkensmodel van chronische myocardischemie als gevolg van progressieve kransslagaderstenose, resulterend in een verminderde hartfunctie zonder infarct. Na ischemie ondergaan dieren een off-pump coronaire bypass-transplantatie met epicardiale plaatsing van van stamcellen afgeleide met exosomen beladen collageenpleister. Deze aanvullende therapie verbetert de myocardfunctie en het herstel.

Chirurgisch varkensmodel van chronische myocardischemie behandeld door met exosomen beladen collageenpleister en off-pump coronaire bypasstransplantaat

Leer een protocol voor het isoleren van enkele kernen uit coronaire endarterectomieweefsel van CABG-patiënten. Deze methode behoudt nucleaire integriteit en ondersteunt transcriptoomanalyse van instabiele atherosclerotische plaques.

Isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten

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