Name: Isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten (Video) | JoVE Journal
Uploaded: 2026-04-03T13:08:00.000+00:00
Duration: 9 min 9 s
Description: Leer een protocol voor het isoleren van enkele kernen uit coronaire endarterectomieweefsel van CABG-patiënten. Deze methode behoudt nucleaire integriteit en ondersteunt transcriptoomanalyse van instabiele atherosclerotische plaques.

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</ol>

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<table><tbody><tr><td>100 &mu;m strainer</td><td>Miltenyi Biotech&nbsp;</td><td>130-098-463</td><td></td></tr><tr><td>30 &mu;m strainer</td><td>Miltenyi Biotech&nbsp;</td><td>130-098-458</td><td></td></tr><tr><td>Bovine Serum Albumin</td><td>HiMedia</td><td>TC546-25G</td><td></td></tr><tr><td>Centrifuge</td><td>MPW</td><td>NA</td><td>https://mpw.pl/en/offer/mpw-352r</td></tr><tr><td>Compound Microscope</td><td>Nikon</td><td>NA&nbsp;</td><td>Nikon Eclipse Ts2 inverted microscope (https://www.microscope.healthcare.nikon.com/en_AOM/products/inverted-microscopes/eclipse-ts2)</td></tr><tr><td>Confocal Microscope&nbsp;</td><td>Nikon</td><td>NA</td><td>Nikon Ti2-E inverted confocal microscope (https://www.microscope.healthcare.nikon.com/en_AOM/products/inverted-microscopes/eclipse-ti2-series)</td></tr><tr><td>Coverslips</td><td>HiMedia</td><td>BG011C</td><td></td></tr><tr><td>CRYOCHIL Wide Mouth Specimen Vial</td><td>Tarsons</td><td>883191</td><td></td></tr><tr><td>DAPI</td><td>Sigma</td><td>D9542-5MG</td><td></td></tr><tr><td>DPBS</td><td>Gibco (Thermofischer)</td><td>10010-023</td><td></td></tr><tr><td>Centrifuge Tube (15 mL)</td><td>Tarsons</td><td>546021</td><td></td></tr><tr><td>Flow cytometer&nbsp;</td><td>BD Biosciences</td><td>663029</td><td>BD FACSLyric (3L12C Instrument CEIVD)</td></tr><tr><td>Hemocytometer</td><td>Axibio</td><td>CCB 100</td><td></td></tr><tr><td>Microcentrifuge Tube (1.5 mL)</td><td>Tarsons</td><td>500010C</td><td></td></tr><tr><td>Microcentrifuge Tube (5 mL)</td><td>Tarsons</td><td>500050</td><td></td></tr><tr><td>MgCl2</td><td>Sigma</td><td>M1028-100ML</td><td></td></tr><tr><td>Micro pestle</td><td>Tarsons</td><td>160020</td><td></td></tr><tr><td>NaCl</td><td>Sigma</td><td>S9888-500G</td><td></td></tr><tr><td>NP-40</td><td>HiMedia</td><td>MB143-100ML</td><td></td></tr><tr><td>Nuclease free water</td><td>Gibco (Thermofischer)</td><td>W4502-1L</td><td></td></tr><tr><td>RNase inhibitor</td><td>Sigma</td><td>333540201</td><td></td></tr><tr><td>RPMI 1640</td><td>Gibco (Thermofischer)</td><td>11875093</td><td></td></tr><tr><td>Slide&nbsp;</td><td>Riviera</td><td>72910135</td><td></td></tr><tr><td>Tris-HCl</td><td>Sigma</td><td>T2194-100ML</td><td></td></tr><tr><td>Trypan blue&nbsp;</td><td>Sigma</td><td>302643-25G</td><td></td></tr></tbody></table>
```

isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten

Single Nuclei Isolation from Coronary Endarterectomy Tissue of Coronary Artery Bypass Graft Patients

Isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten

Atherosclerosis is a chronic, progressive inflammatory condition characterized by the accumulation of plaque in the walls of arteries. During the ...

single-nuclei-isolation-from-coronary-endarterectomy-tissue-coronary

Research

JoVE Journal

Biology

Biologie

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Isolatie van cardiomyocyten Kernen van Post-mortem weefsel

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Isolatie en functionele karakterisering van Human ventriculaire cardiomyocyten van Fresh Chirurgische Samples

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Isolatie van muis coronaire endotheelcellen

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Isolatie van Perivasculaire Multipotent Precursor Cell Populaties van de Mens Cardiac Tissue

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Hoewel uitdagend, is de isolatie van pulmonale endotheelcellen essentieel voor studies naar longontsteking. Dit protocol beschrijft een procedure voor de hoogproductieve, zeer zuivere isolatie van macrovasculaire en microvasculaire endotheelcellen.

Microvasculaire en macrovasculaire endotheliale celisolatie en zuivering van longmonsters

Hier presenteren we een protocol dat de voorbereiding van celkernen beschrijft. Na microdissectie en enzymatische dissociatie van hartweefsel in afzonderlijke cellen, werden de voorlopercellen bevroren, gevolgd door isolatie van zuivere levensvatbare cellen, die werden gebruikt voor single-nucleus RNA-sequencing en de single-nucleus assay voor transposase-toegankelijk chromatine met high-throughput sequencing-analyses.

Kernisolatie van hartvoorlopercellen van muizen voor epigenoom- en genexpressieprofilering met eencellige resolutie

Leer een protocol voor het isoleren van enkele kernen uit coronaire endarterectomieweefsel van CABG-patiënten. Deze methode behoudt nucleaire integriteit en ondersteunt transcriptoomanalyse van instabiele atherosclerotische plaques.

Isolatie van enkele kernen uit het coronaire endarterectomieweefsel van coronaire bypass-transplantatiepatiënten

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