Name: Amikacine Beschermingsassay voor Kwantificering en Visualisatie van Escherichia coli-celinvasie (Video) | JoVE Journal
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Description: Leer een amikacine-gebaseerde beschermingsassay voor kwantificering en visualisatie van Escherichia coli-invasie in epitheelcellen. Deze methode biedt een betrouwbaar alternatief voor gentamicine bij antibiotica-resistente isolaten.

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<ol>
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<table><tbody><tr><td>75 cm2 Tissue culture flask</td><td>Fisher Scientific&nbsp;</td><td>FB012937</td><td></td></tr><tr><td>Amikacin disulfate&nbsp;</td><td>Research Products International&nbsp;</td><td>A20540-25.0</td><td></td></tr><tr><td>Antibiotic-Antimycotic (100x)</td><td>Gibco</td><td>15240062</td><td></td></tr><tr><td>Anti-E.coli antibody</td><td>Abcam</td><td>ab137967</td><td></td></tr><tr><td>BioLite microwell plates - 6 well</td><td>Fisher Scientific&nbsp;</td><td>12-556-004</td><td></td></tr><tr><td>Bovine serum albumin</td><td>Sigma-Aldrich</td><td>A7906</td><td></td></tr><tr><td>Collagen I, Rat tail, 100 mg</td><td>Corning</td><td>354236</td><td></td></tr><tr><td>Countess cell counting chamber slide</td><td>Invitrogen</td><td>C10312</td><td></td></tr><tr><td>DMEM, high glucose</td><td>Gibco</td><td>11965118</td><td></td></tr><tr><td>Fetal bovine serum</td><td>Corning</td><td>35-011-CV</td><td></td></tr><tr><td>Galcial acetic acid</td><td>Sigma-Aldrich</td><td>A6283</td><td></td></tr><tr><td>Glycine ultrapure</td><td>USBiological</td><td>G8165</td><td></td></tr><tr><td>Goat anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488</td><td>Invitrogen</td><td>A-11008</td><td></td></tr><tr><td>Hoechst 33342, trihydrochloride trihydrate</td><td>Invitrogen</td><td>H3570</td><td></td></tr><tr><td>Liofilchem&nbsp;MTS amikacin [AK] 0.016-256 &mu;g/mL</td><td>Fisher Scientific&nbsp;</td><td>22-777-704</td><td>Amikacin MIC Test Strips</td></tr><tr><td>Paraformaldehyde solution, 4% in PBS</td><td>Fisher Scientific&nbsp;</td><td>J19943.K2</td><td></td></tr><tr><td>PBS, 1x</td><td>Corning</td><td>21-040-CV</td><td></td></tr><tr><td>Poly-L-lysine coated sterile German glass coverslips for cell culture</td><td>Neuvitro</td><td>NC1327375</td><td></td></tr><tr><td>ProLong gold antifade mountant&nbsp;</td><td>Invitrogen</td><td>P36934</td><td></td></tr><tr><td>Rhodamine-Phalloidin</td><td>Invitrogen</td><td>R415</td><td>Red-orange 540/565 nm</td></tr><tr><td>Surface treated sterile tissue culture plates</td><td>Fisher Scientific&nbsp;</td><td>FB012929</td><td>24-well plates</td></tr><tr><td>Triton X-100 (Electrophoresis)</td><td>Fisher Scientific&nbsp;</td><td>BP151-100</td><td></td></tr><tr><td>Trypan blue</td><td>Invitrogen</td><td>T10282</td><td></td></tr><tr><td>Trypsin-EDTA (0.25%), phenol red</td><td>Gibco</td><td>25200056</td><td></td></tr><tr><td>Tween-20</td><td>Fisher Scientific&nbsp;</td><td>BP337-100</td><td></td></tr></tbody></table>

amikacine beschermingsassay voor kwantificering en visualisatie van escherichia coli-celinvasie

Amikacin Protection Assay for Quantification and Visualization of Escherichia coli Cell Invasion

Amikacine Beschermingsassay voor Kwantificering en Visualisatie van Escherichia coli-celinvasie

Epithelial cell invasion is a key phenotype for many enteric pathogens, including Salmonella, Shigella, and Vibrio. An emerging invasive pathogen is ...

amikacin-protection-assay-for-quantification-visualization

Research

JoVE Journal

Immunology and Infection

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Amikacine Beschermingsassay voor Kwantificering en Visualisatie van Escherichia coli-celinvasie

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