Name: flame emission spectrometry basics
Uploaded: 2024-12-05T18:32:16.000Z
Duration: 1 min 2 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Earth and Space Science

Space Systems

3.3.9-12.C

Flame Photometry: Overview

Fotometria płomieniowa lub spektrometria emisji płomienia wykorzystuje płomień do wzbudzenia roztworu próbki.
Płomienie powinny posiadać energię wystarczającą do atomizacji, niską emisję i absorpcję przy odpowiednich długościach fal oraz zdolność do działania przy niskich prędkościach gazu.
Zwykle stosuje się płomienie propanowo-powietrzne, ponieważ płomienie butanowo-powietrzne i gazowo-powietrzne są mniej wrażliwe ze względu na niższe temperatury.
Fotometry płomieniowe wykorzystują system nebulizatora-palnika do przekształcania roztworu próbki w mgłę lub aerozol. Istnieją dwa główne typy palników.
Palniki z mieszanką wstępną lub przepływem laminarnym wytwarzają delikatną mgiełkę w komorze parowania, która jest następnie mieszana z paliwem i gazami utleniającymi przed dotarciem do głowicy palnika.
Palniki o całkowitym zużyciu lub przepływie turbulentnym składają się z trzech koncentrycznych rur, które oddzielnie przenoszą roztwór próbki, paliwo gazowe i gaz utleniający, umożliwiając ich mieszanie tylko na końcówce palnika.

fotometria płomieniowa: przegląd

Fotometria płomieniowa, znana również jako spektrometria emisyjna płomienia, to technika stosowana do jakościowej i ilościowej analizy pierwiastków obecnych w próbce, wykorzystująca płomień jako źródło energii wzbudzenia. Koncepcja fotometrii płomieniowej została zrealizowana na początku lat 60. XIX wieku przez Kirchhoffa i Bunsena, którzy odkryli, że określone pierwiastki emitują charakterystyczne promieniowanie, gdy są pobudzane w płomieniach. Pierwszy przyrząd opracowany w tym celu został użyty do pomiaru sodu (Na) w popiele roślinnym przy użyciu płomienia Bunsena. Jednak wyzwaniem było znalezienie najskuteczniejszego sposobu wprowadzenia próbki do płomienia. Dopiero w 1929 roku, kiedy Lundegardh wprowadził nebulizator, dokonano znaczącego przełomu, umożliwiającego powtarzalne wprowadzenie próbki do płomienia.

 W spektrometrii emisyjnej płomienia nebulizator przekształca próbkę cieczy w drobną mgiełkę lub aerozol. Osiąga się to poprzez przepuszczenie strumienia gazu pod wysokim ciśnieniem nad końcem rurki kapilarnej zawierającej próbkę i zasysanie jej do komory rozpyłowej. Aerozol wytworzony następnie trafia do palnika, gdzie ciepło płomienia usuwa rozpuszczalnik, tworząc suche cząstki, które ulegają lotnieniu i wytwarzają wolne atomy do analizy.

 Wczesne instrumenty wykorzystywały kwarcowe spektrografy pryzmatyczne i rejestrację fotograficzną do rozpraszania i wychwytywania linii emisyjnych atomów. Jednak postępy w zakresie filtrów optycznych i elektrycznych fotodetektorów zastąpiły te komponenty, poprawiając precyzję i wygodę.

 Fotometria płomieniowa polega na wprowadzeniu roztworu próbki do nebulizatora, który zamienia go w drobną mgiełkę lub aerozol. Rozpylona próbka wchodzi następnie do płomienia wraz z powietrzem lub tlenem i gazem paliwowym, takim jak propan lub acetylen. Płomień zapewnia wzbudzenie termiczne niezbędne do pobudzenia atomów w próbce. Gdy te wzbudzone atomy się rozluźniają, ich emitowane promieniowanie jest wykrywane przez fotokomórkę lub fotopowielacz.

 Fotometria płomieniowa jest szczególnie skuteczna w pomiarze pierwiastków sodu, potasu, litu i wapnia. Płomień używany w fotometrii płomieniowej to zazwyczaj płomień propan-powietrze o zakresie temperatur 1900-2000 °C, chociaż można również stosować alternatywne płomienie, takie jak butan-powietrze lub gaz ziemny-powietrze. Fotometria płomieniowa ma pewne ograniczenia, które można pokonać, stosując wyższe temperatury i bardziej redukujące płomienie, takie jak powietrze-acetylen, wraz z detekcją spektrometryczną o wyższej rozdzielczości. Jednak te podejścia nie są konkurencyjne cenowo w porównaniu z szerzej stosowaną techniką absorpcyjnej spektrometrii atomowej płomieniowej lub AAS.

Fotometria płomieniowa: Przegląd

Fotometria płomieniowa, znana również jako spektrometria emisji płomienia, jest techniką stosowaną do analizy jakościowej i ilościowej pierwiastków obecnych w ...

Education

JoVE Core

Analytical Chemistry

Chemia analityczna

Unit 1

Jednostka 1

Atomic Spectroscopy

Spektroskopia atomowa

KEY TERMS AND CONCEPTS

KLUCZOWE TERMINY I POJĘCIA

flame emission spectrometry basics

Flame emission spectrometry uses a flame to excite elements in a sample so their light can be measured. It is also called flame photometry. The method can be used for both qualitative and quantitative analysis of elements.

The idea became established in the early 1860s when Kirchhoff and Bunsen showed that specific elements give off characteristic radiation in a flame. The first instrument for this purpose measured sodium in plant ash with a Bunsen flame. A major problem was how to add the sample to the flame in a steady way.

That problem changed in 1929, when Lundegardh introduced a nebulizer. A nebulizer turns a liquid sample into a fine mist, or aerosol. A high-pressure gas stream passes over a capillary tube, draws the sample into a spray chamber, and sends the aerosol to the burner.

At the burner, flame heat removes the solvent and leaves dry particles. These particles volatilize and form free atoms for analysis. Early instruments used quartz prism spectrographs and photographic recording to separate and capture the atomic emission lines. Later, optical filters and electrical photodetectors replaced those parts and made the method more precise and easier to use.

In a flame photometry setup, the sample solution enters the nebulizer and then the flame with air or oxygen and a fuel gas such as propane or acetylene. The flame supplies the thermal energy needed to excite the atoms. When the atoms return to a lower energy state, they emit radiation that is measured by a photocell or photomultiplier.

This method is especially useful for sodium, potassium, lithium, and calcium. A propane-air flame is commonly used and reaches about 1900 to 2000 degrees Celsius. Butane-air and natural gas-air flames can also be used. Some limits can be reduced with higher temperatures and more reducing flames, such as air-acetylene, together with higher-resolution spectrometric detection. Even so, these options are not as cost-competitive as flame atomic absorption spectrometry, also called AAS.

Flame Emission Spectrometry Basics

atomic spectroscopy: absorption, emission, and fluorescence

atomic-spectroscopy-absorption-emission-and-fluorescence

Element Analysis with Atomic Light Spectra

Atomic spectroscopy is used to identify and measure elements by their light signals. It supports both qualitative analysis, which tells what is present, and quantitative analysis, which tells how much is present. The optical methods in this group are atomic absorption spectroscopy, atomic emission spectroscopy, and atomic fluorescence spectroscopy. These methods begin with atomization. In this step, a solid, liquid, or solution sample is changed into gas-phase atoms and ions. Once the sample...

atomic spectroscopy: effects of temperature

atomic-spectroscopy-effects-of-temperature

How Heat Changes Atomic Spectra

Heat changes atomic spectra by affecting atomization, excitation, ionization, and line shape. In atomic spectroscopy, atomization means converting a sample into gas-phase atoms and ions. The flame temperature needed for this step changes how efficiently the method works and shifts the balance between excited and ground-state atoms. At thermal equilibrium, the relative numbers of excited and ground-state atoms can be estimated with the Maxwell-Boltzmann distribution. A small temperature rise...

atomic absorption spectroscopy: overview

Atomic Absorption Spectroscopy for Trace Metals

Atomic absorption spectroscopy (AAS) measures trace metals by tracking how atoms absorb electromagnetic radiation (EMR). The absorbed light makes atoms move to a higher-energy orbit. In AAS, the key step is atomization, which converts the sample into gas-phase atoms. A flame or a furnace is typically used for this step. Inside the flame, some atoms become thermally excited. Most stay in the ground state, which is the lowest-energy state. These ground-state gas-phase atoms absorb EMR only when...

atomic absorption spectroscopy: instrumentation

atomic-absorption-spectroscopy-instrumentation

Atomic Absorption Spectroscopy: Key Parts

Atomic absorption spectrophotometry uses a set of key parts to measure light absorbed by atoms. The main components are a radiation source, an atomizer, a monochromator, and a detector. Each part has a specific job in the instrument. The radiation source is usually a hollow-cathode lamp, or HCL, or an electrodeless-discharge lamp, or EDL. Both produce a narrow emission line at the needed wavelength. Some instruments instead use continuum sources with high-resolution monochromators to narrow...

atomic absorption spectroscopy: radiation and light sources

atomic-absorption-spectroscopy-radiation-and-light-sources

Choosing Light Sources for AAS Measurements

Atomic absorption spectroscopy (AAS) uses a light source that emits a very narrow range of wavelengths. This is needed because the source must match the absorption behavior of the analyte atom. The choice of source also depends on giving a precise, intense emission at the right wavelength for accurate detection. Two common line sources in AAS are hollow-cathode lamps (HCLs) and electrodeless-discharge lamps (EDLs). These lamps are selected based on the element being analyzed and the level of...

atomic absorption spectroscopy: atomization methods

atomic-absorption-spectroscopy-atomization-methods

Atomic Absorption Spectroscopy: Sample Atomization

Atomic absorption spectroscopy uses sample atomization to turn a liquid specimen into free atoms for analysis. The two main approaches are flame atomization and electrothermal atomization. Both methods prepare the sample so its atoms can be measured by the instrument. Flame atomization uses a nebulizer and spray chamber to mix the sample with a fuel-oxidant mixture. This creates a fine aerosol mist that enters the burner. The fuel and oxidant are usually close to a stoichiometric ratio, but a...

atomic absorption spectroscopy: interference

atomic-absorption-spectroscopy-interference

Correcting Errors in Atomic Absorption Spectroscopy

Atomic absorption spectroscopy interference can create systematic error by making the analytical signal or the background look stronger or weaker than it should be. In atomic absorption measurements, these errors are usually grouped as spectral interference, chemical interference, and physical interference. Spectral interference happens when signals from other elements or molecules overlap with the analyte signal, the substance being measured. This overlap can falsely raise or hide the analyte...

atomic absorption spectroscopy: lab

AAS Sample Prep and Calibration

Atomic absorption spectroscopy (AAS) depends on careful sample preparation and calibration. Samples must be turned into clear solutions before measurement. That often means extra treatment to dissolve materials such as soils, animal tissues, and minerals. Common sample prep methods include hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, and fusion with reagents. These steps help make the sample suitable for AAS analysis. Solutions that contain organic...

atomic emission spectroscopy: overview

Atomic Emission Spectroscopy for Element Analysis

Atomic emission spectroscopy is used to identify the elements in a sample by measuring the light they emit. Atoms are first excited to higher energy levels by thermal energy from hot sources such as plasma, arcs, or sparks. When they fall back to lower energy levels, they give off light at specific wavelengths. Each element produces its own set of lines, so the emission spectrum can show both what is present and how much is there. AES instruments are similar to atomic absorption spectrometers,...

atomic emission spectroscopy: instrumentation

atomic-emission-spectroscopy-instrumentation

AES Sample Handling and Detection

Atomic emission spectrometry (AES) uses sample handling, atomization, and detection parts to measure emitted light from atoms and ions. The sample must first be changed into a gas-phase form so it can produce a measurable signal. AES uses two main atomizer types. Continuous atomizers, such as flames and plasmas, feed the sample in a steady stream. Discrete atomizers add one sample at a time with syringes or autosamplers. The electrothermal atomizer is the most common discrete type. Many...

atomic emission spectroscopy: interference

atomic-emission-spectroscopy-interference

Reducing Interference in AES Signals

Atomic emission spectroscopy (AES) can produce interfering signals when atoms and molecules in a flame or plasma emit overlapping light. High-temperature atomizers excite many species at once, including oxides, hydroxides, and flame combustion products. These extra emissions can make the analyte signal harder to read. Spectral interference happens when emission lines or bands overlap. One way to reduce it is to improve instrument resolution. Another is to choose a different emission line for...

inductively coupled plasma atomic emission spectroscopy: principle

inductively-coupled-plasma-atomic-emission-spectroscopy-principle

ICP-AES Torch and Sample Analysis

Inductively coupled plasma atomic emission spectroscopy uses a plasma torch to analyze elements in a sample. This method is also called inductively coupled plasma optical emission spectroscopy, or ICP-OES. It is widely used because the plasma creates a strong environment for elemental analysis. The ICP source, or torch, contains three concentric quartz tubes. Argon gas flows through the tubes. A spark from a Tesla coil starts the ionization of argon and creates a high-temperature plasma. The...

inductively coupled plasma atomic emission spectroscopy: instrumentation

inductively-coupled-plasma-atomic-emission-spectroscopy

ICP-AES Instrument Types and Detection

Inductively coupled plasma atomic emission spectroscopy, or ICP-AES, uses an inductively coupled plasma to detect and analyze elements in a sample. The method measures the light given off by atoms, which helps identify and compare different elements. It is a common approach in atomic emission spectroscopy. ICP-AES instruments are built in three main forms: sequential, simultaneous multichannel, and Fourier transform instruments. Fourier transform designs are less commonly used, but they are...

atomic emission spectroscopy: lab

Plasma Sources in Atomic Emission Spectroscopy

Atomic emission spectroscopy uses plasma sources to identify elements by their light. The method creates abundant spectra with characteristic emission lines. Inductively coupled plasma, or ICP, is especially useful because it gives strong quantitative data. It works well under stable conditions, with low noise, low background, and few interferences. Newer air-operated microwave sources are also being studied. These sources may be more cost-effective than conventional ICP systems. Atomic...

atomic fluorescence spectroscopy

Measuring Elements by Atomic Fluorescence

Atomic fluorescence spectroscopy measures elements by detecting the light atoms give off after they are excited by electromagnetic radiation. The atoms are first placed in a flame, furnace, or plasma. They absorb energy, become excited, and then release that energy as they return to their original state. The emitted light is called fluorescence. The fluorescence is observed at a right angle to the incoming beam. This setup helps separate the emitted light from the light used to excite the...

flame photometry: overview

Flame emission spectrometry uses a flame to excite elements in a sample so their light can be measured. It is also called flame photometry. The method can be used for both qualitative and quantitative analysis of elements. The idea became established in the early 1860s when Kirchhoff and Bunsen showed that specific elements give off characteristic radiation in a flame. The first instrument for this purpose measured sodium in plant ash with a Bunsen flame. A major problem was how to add the...

flame photometry: lab

Flame Photometry and Metal Detection

Flame photometry is a method for detecting metals by measuring the light they emit in a flame. A solution such as potassium chloride is aspirated into the flame. The solvent evaporates first. The remaining salt becomes dehydrated and then dissociates into free gaseous atoms in the ground state. Some of these atoms absorb energy from the flame and move to an excited state. When they return to the ground state, they emit photons at characteristic wavelengths. These emissions are very narrow...

key terms and concepts

Learn how flame emission spectrometry uses a nebulizer, flame excitation, and photodetectors to measure elements like sodium, potassium, lithium, and calcium.

Flame Emission Spectrometry Basics | JoVE K12

analytical chemistry

JoVE Core Analytical Chemistry simplifies complex chemical principles by breaking them down into clear, engaging animations, making topics from SI Units to NMR spectroscopy more accessible and straightforward. Additionally, the scientist-in-action videos highlight the application of these concepts in real-world research conducted in laboratories globally.

Arizona Science Standards

High School

Physical Science

HS+C.P1

HS+C.P1U1.2

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3.3.9-12.A

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3.3.9-12.B

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3.3.9-12.D

Flame Emission Spectrometry Basics

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