Kultura komórek macierzystych

Stem cell culture is the process of growing stem cells in vitro under optimal conditions.

Stem cells have a wide range of medical applications like tissue and organ regeneration.

Stem cell samples are usually extracted from adult  or embryonic tissues.

These samples are then transferred to plastic culture dishes coated with a feeder layer consisting of inactivated mouse embryonic fibroblast cells, which provide necessary intercellular interactions and nutrients;  and prevent the spontaneous differentiation of stem cells.

The culture dish is supplemented with media primarily containing growth hormones and essential nutrients. It nourishes stem cells to divide and spread over the culture dish, forming colonies.

Once grown to the required size, these colonies are removed gently and subjected to the passage, where they are further replated or subcultured multiple times to establish a pure and continuously growing cell line.

An established cell line can give rise to millions of stem cells in vitro. So, a batch of these cultured stem cells can be frozen for future use.