Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Podsumowanie

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Streszczenie

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Protokół

Opublikowano sprostowanie do artykułu Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

Zapasy ludzkich komórek RCTEC/RCTE użyte tutaj oraz w pracach Caralt et al., 2015, Uzarski et al., 2015 zostały pierwotnie przekazane przez dr. Loghmana-Adhama (wówczas z Uniwersytetu w St. Louis) współautorowi, dr. Wandinger-Ness (Uniwersytet Nowego Meksyku), na mocy umowy MTA w 2001 roku. Unieśmielone SV40 ludzkie komórki RCTEC/RCTE zostały scharakteryzowane jako linia komórkowa cewek dystalnych, zgodnie z opisem w Loghman-Adham et al., 2003. Na podstawie niedawnego sekwencjonowania DNA metodą krótkich tandemowych powtórzeń (STR) (wykonanego przez IDEXX BioResearch) wczesnych pasaży zapasów komórek RCTEC/RCTE okazało się, że komórki te mają pochodzenie mieszane. Dalsze analizy produktów PCR przy użyciu kapilarnej elektroforezy QIAxcel wykazały obecność produktu psiego. Produkt ten został zsekwencjonowany i zidentyfikowany jako pochodzący od psa. Zestaw markerów STR specyficznych dla psów został porównany z próbką, co wykazało, że próbka posiada profil genetyczny z 92% tożsamością z linią komórkową MDCK.  Późniejsze zapasy komórek wykorzystane w niniejszej publikacji zostały poddane profilowaniu STR, które wykazało dryf do 100% linii MDCK. Pomimo psiego pochodzenia, a nie ludzkiego, jak wcześniej sądzono, MDCK jest również linią nabłonkowych komórek cewek dystalnych. Z tego powodu wcześniejsze interpretacje i wnioski sformułowane z wykorzystaniem tych komórek tutaj oraz w Caralt et al., 2015, Uzarski et al., 2015 pozostają aktualne, jednak w imię rygoru, powtarzalności i walidacji eksperymentalnej przez innych badaczy, informujemy o tej błędnej identyfikacji. Informacja ta zostanie również zgłoszona do bazy danych Międzynarodowego Komitetu Uwierzytelniania Linii Komórkowych (ICLAC) (http://iclac.org).