Celem przedstawionej tutaj metody jest pokazanie, jak mikromacierze mikrośrodowiskowe (MEMA) mogą być wytwarzane i wykorzystywane do badania wpływu tysięcy prostych mikrośrodowisk kombinatorycznych na fenotyp hodowanych komórek.
Method Article
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| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| Aushon 2470 | Aushon BioSystems | Arrayer używany w protokole | |
| Nikon HCA | System obrazowania o wysokiej zawartości firmy Nikon zaprojektowany w oparciu o mikroskop odwrócony Nikon Eclipse Ti | ||
| EDTA | Invitrogen | 15575-038 | |
| Glicerol | Sigma | G5516 | |
| Triton X100 | Sigma | T9284 | |
| Tween 20 | Sigma | P7949 | |
| Kolliphor P338 | BASF | 50424591 | |
| 384-dołkowa płytka mikromatrycowa, cylindryczna Thermo | Fisher | ab1055 | |
| Nunc 8 dobrze naczynie | Thermo Fisher | 267062 | |
| Roztwór paraformaldehydu 16% | Mikroskopia elektronowa Science | 15710 | |
| BSA | Fisher | BP-1600 | |
| Sondy molekularne | maską sodową | ||
| DAPI | Promo Kine | PK-CA70740043 | |
| ALCAM | R & D Systems | 656-AL | ECM |
| Cadherin-20 (CDH20) | R & D Systems | 5604-CA | ECM |
| Cadherin-6 (CDH6) | R & D Systems | 2715-CA | ECM |
| Cadherin-8 (CDH8) | R & D Systems | 188-C8 | ECM |
| CD44 | R & D Systems | 3660-CD | ECM |
| CEACAM6 | R & D Systems | 3934-CM | |
| ECM Kolagen I | Cultrex | 3442-050-01 | ECM |
| Kolagen typu II | Millipore | CC052 | ECM |
| Kolagen typu III | Milipory | CC054 | ECM |
| Kolagen typu IV | Sigma | C5533 | ECM |
| Kolagen typu V | Millipore | CC077 | ECM |
| COL23A1 | R & D Systems | 4165-CL | ECM |
| Desmoglein 2 | R & D Systems | 947-DM | ECM |
| E-kadheryna (CDH1) | R & D Systems | 648-EC | ECM |
| ECM1 | R & D Systems | 3937-EC | ECM |
| Fibronektyna | R & D Systems | 1918-FN | ECM |
| GAP43 | Abcam | ab114188 | ECM |
| HyA-500K | R & D Systems | GLR002 | ECM |
| HyA-50K | R & D Systems | GLR001 | ECM |
| ICAM-1 | R & D Systems | 720-IC | ECM |
| Laminin | Sigma | L6274 | ECM |
| Laminin-5 | Abcam | ab42326 | ECM |
| Lumican | R & D Systems | 2846-LU | ECM |
| M-CAD (CDH15) | R & D Systems | 4096-MC | ECM |
| Nidogen-1 | R & D Systems | 2570-ND | ECM |
| Osteoadheryna/OSAD | R & D Systems | 2884-AD | ECM |
| Osteopontyna (SPP) | R & D Systems | 1433-OP ECM | |
| P-Kadheryna (CDH3) | R & D Systems | 861-PC | ECM |
| PECAM1 | R & D Systems | ADP6 | ECM |
| Tenascin C | R & D Systems | 3358-TC | ECM |
| VCAM1 | R & D Systems | ADP5 | ECM |
| Witronektyna | R & D Systems | 2308-VN | ECM |
| Biglycan | R & D Systems | 2667-CM | ECM |
| Decorin | R & D Systems | 143-DE | ECM |
| Periostin | R & D Systems | 3548-F2 | ECM |
| SPARC/osteonektyna | R & D Systems | 941-SP | ECM |
| Trombospondyna-1/2 | R & D Systems | 3074-TH | ECM |
| Brevican | R & D Systems | 4009-BC | ECM |
| Elastyna | BioMatrix | 5052 | ECM |
| Fibrylina | Lynn Sakai Lab OHSU | NIE DOTYCZY | ECM |
| ANGPT2 | RnD_Systems_Own | 623-AN-025 | Ligand |
| IL1B | RnD_Systems_Own | 201-LB-005 | Ligand |
| CXCL8 | RnD_Systems_Own | 208-IL-010 | Ligand |
| IGF1 | RnD_Systems_Own | 291-G1-200 | Ligand |
| TNFRSF11B | RnD_Systems_Own | 185-OS | Ligand |
| BMP6 | RnD_Systems_Own | 507-BP-020 | Ligand |
| FLT3LG | RnD_Systems_Own | 308-FK-005 | Ligand |
| CXCL1 | RnD_Systems_Own | 275-GR-010 | Ligand |
| DLL4 | RnD_Systems_Own | 1506-D4-050 | Ligand |
| HGF | RnD_Systems_Own | 294-HGN-005 | Ligand |
| Wnt5a | RnD_Systems_Own | 645-WN-010 | Ligand |
| CTGF | Life_Technologies_Own | PHG0286 | Ligand |
| LEP | RnD_Systems_Own | 398-LP-01M | Ligand |
| FGF2 | Sigma_Aldrich_Own SRP4037-50UG | Ligand | |
| FGF6 | RnD_Systems_Own | 238-F6 | Ligand |
| IL7 | RnD_Systems_Own | 207-IL-005 | Ligand |
| TGFB1 | RnD_Systems_Own | 246-LP-025 | Ligand |
| PDGFB | RnD_Systems_Own | 220-BB-010 | Ligand |
| WNT10A | Genemed_Own | 90009 | Ligand |
| PTN | RnD_Systems_Own | 252-PL-050 | Ligand |
| BMP3 | RnD_Systems_Own | 113-BP-100 | Ligand |
| BMP4 | RnD_Systems_Own | 314-BP-010 | Ligand |
| TNFSF11 | RnD_Systems_Own | 390-TN-010 | Ligand |
| CSF2 | RnD_Systems_Own | 215-GM-010 | Ligand |
| BMP5 | RnD_Systems_Own | 615-BMC-020 | Ligand |
| DLL1 | RnD_Systems_Own | 1818-DL-050 | Ligand |
| NRG1 | RnD_Systems_Own | 296-HR-050 | Ligand |
| KNG1 | RnD_Systems_Own | 1569-PI-010 | Ligand |
| GPNMB | RnD_Systems_Own | 2550-AC-050 | Ligand |
| CXCL12 | RnD_Systems_Own | 350-NS-010 | Ligand |
| IL15 | RnD_Systems_Own | 247-ILB-005 | Ligand |
| TNF | RnD_ Systems_Own | 210-TA-020 | Ligand |
| IGFBP3 | RnD_Systems_Own | 675-B3-025 | Ligand |
| WNT3A | RnD_Systems_Own | 5036-WNP-010 | Ligand |
| PDGFAB | RnD_Systems_Own | 222-AB | Ligand |
| AREG | RnD_Systems_Own | 262-AR-100 | Ligand |
| JAG1 | RnD_Systems_Own | 1277-JG-050 | Ligand |
| BMP7 | RnD_Systems_Own | 354-BP-010 | Ligand |
| TGFB2 | RnD_Systems_Own | 302-B2-010 | Ligand |
| VEGFA | RnD_Systems_Own | 293-VE-010 | Ligand |
| IL6 | RnD_Systems_Own | 206-IL-010 | Ligand |
| CXCL12 | RnD_Systems_Own | 351-FS-010 | Ligand |
| NRG1 | RnD_Systems_Own | 378-SM | Ligand |
| IGFBP2 | RnD_Systems_Own | 674-B2-025 | Ligand |
| SHH | RnD_Systems_Own | 1314-SH-025 | Ligand |
| FASLG | RnD_Systems_Own | 126-FL-010 | Ligand |
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