Fluorochrome Labeling
Fluorochrome Labeling - Related Videos
07:29
Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
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Cited by 32 •
2010
Undecalcified bone histology provides important information for a variety of clinical and research applications. It is technically challenging, particularly with large size specimens. This video illustrates the process of producing good quality sections and demonstrates the technical difficulties and methods with which to overcome them.
10:58
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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2019
Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
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Cited by 3 •
2025
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
03:59
Bromodeoxyuridine Pulse Labelling Assay: A Technique to Measure Cell Proliferation
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2023
The video describes the stepwise pulse labeling assay of mammalian cells with bromodeoxyuridine (BrdU) for measuring cell proliferation. The BrdU uptake permits the temporal tracking of cells that were in the synthesis phase at a specific point in time, without requiring the cells to be synchronized.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
A fluorochrome absorbs energy from light at a suitable excitation wavelength and then releases part of that energy as emitted light at a longer wavelength. This wavelength shift allows light-based instruments to separate the emitted signal from the incoming illumination, making labeled cells, biomolecules, or probes detectable and permitting their locations to be examined.
Target selectivity comes from two main strategies: chemical coupling attaches the dye to a chosen biomolecule, while affinity-based binding connects the label through a recognition interaction. These approaches allow fluorochrome labeling to mark antibodies, nucleic acids, proteins, cells, or molecular probes according to the biological question being investigated.
The excitation wavelength determines whether the fluorochrome can absorb light energy efficiently enough to produce an observable emission signal. Using an appropriate wavelength therefore supports reliable detection and localization of the labeled target. In practice, the selected illumination must match the properties of the dye and the capabilities of the light-based instrument used for observation.
Fluorochrome labeling converts the presence or location of selected targets into detectable light signals. Because labels can be associated with different cells, biomolecules, or probes, the method can reveal cellular structures, track molecular interactions, and help distinguish cell populations. Its multiplexing potential further supports examining multiple biological features within the same investigative framework.
A typical workflow identifies the biological target, selects a compatible fluorochrome, and associates the dye with the target through chemical coupling or affinity-based binding. The labeled material is then illuminated at an appropriate excitation wavelength, and the emitted light is recorded with a suitable instrument. The resulting signal supports target localization or population analysis.
The approach can be applied to cells, biomolecules, and molecular probes, including antibodies, nucleic acids, and proteins. Fluorescence microscopes detect and localize labeled structures, whereas flow cytometers analyze fluorescent signals from cells and help distinguish populations. These instrument choices connect the labeling strategy to either spatial imaging or cell-based measurement.
Researchers apply it when they need to visualize cellular structures, follow molecular interactions, or separate cell populations according to fluorescent signals. The technique contributes to fluorescence microscopy, flow cytometry, and immunofluorescence, while its sensitivity and multiplexing potential support diagnostics, cell biology, and quantitative research involving labeled biological targets.