Crispr/cas9 Gene Editing
Crispr/cas9 Gene Editing - Related Videos
02:30
A CRISPR-Cas9 Technique for Gene Editing in T Cells
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2025
This video demonstrates an assay for performing gene editing in human T cells using the CRISPR-Cas9 technology. A mixture of primary CD4+ and CD8+ T cells is combined with a CRISPR-Cas9 ribonucleoprotein complex, targeting specific genes for knockout. Upon electroporation, the sgRNA guides Cas9 to the target DNA sequence, creating precise cuts. These cuts are then repaired by the cell's non-homologous end-joining mechanism, leading to gene knockout.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
16:08
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
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Cited by 34 •
2018
A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
FAQs
Guide RNA sequence matching determines where Cas9 makes a double-strand break, so the selected DNA site directly shapes the experiment’s result. In cancer research, targeting a gene associated with tumor growth or drug resistance can reveal how that gene affects cellular behavior. The quality of this targeting step therefore influences whether the edited cells provide interpretable functional evidence.
The intended genetic change determines which repair outcome is valuable. Non-homologous end joining can disrupt a target gene, whereas homology-directed repair can support replacement or more precise modification of the sequence. This distinction allows investigators to choose between loss-of-function studies and experiments designed to recreate or alter specific cancer-associated mutations.
The break converts a selected DNA target into a testable genetic perturbation after cellular repair. If repair disrupts a gene, researchers can examine whether the resulting cells show changes linked to tumor growth, disease mechanisms, or drug response. This connection between a defined DNA alteration and a measurable cellular outcome supports functional analysis of cancer-related genes.
A typical workflow selects a cancer-relevant gene or mutation, designs a guide RNA matching the target DNA, and uses Cas9 to create the break. Researchers then rely on cellular repair to produce disruption, replacement, or modification and examine the resulting cells. Comparing edited outcomes with the original cellular state helps link the genetic change to cancer-related behavior.
Researchers apply CRISPR/Cas9 gene editing when they need to test whether particular genes contribute to tumor growth or broader cancer mechanisms. Targeted changes can alter candidate genes and provide a way to examine their functional effects in cells. These experiments help distinguish genes that are merely associated with cancer from those that actively influence the disease process.
In drug-resistance research, targeted editing helps investigators examine genes that may influence how cancer cells respond to treatment. The same flexibility supports engineering immune cells for potential cancer therapies by modifying selected genetic features. Together, these applications connect gene function with treatment response and therapeutic development, while supporting broader cancer functional genomics.