Nitrocellulose Slide Spotting
Nitrocellulose Slide Spotting - Related Videos
09:17
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
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Cited by 3 •
2017
We report and demonstrate an optimized nitrocellulose binding assay that can be used to quantify autophosphorylation of purified bacterial histidine kinases. Our method has several advantages over traditional SDS-PAGE based techniques, providing a valuable alternative for characterizing these important proteins.
10:45
Finding Your Blind Spot and Perceptual Filling-in
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University In the back of everyone's eye is a small piece of neural tissue called the retina. The retina has photosensitive cells that respond to stimulation by light. The responses of these cells are sent into the brain through the optic nerve, a bundle of neural fibers. In each retina there is a place somewhere in the periphery where the outputs from retinal cells collect and the bundled optic nerve exits to the brain. At that location,...
06:53
SpOT the Correct Tissue Every Time in Multi-tissue Blocks
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Cited by 5 •
2015
The purpose of the Specimen Orientation Tag (SpOT) is to function as an orientation tool to aid in individual tissue identification in multi-tissue paraffin blocks. These protocols demonstrate how it is constructed easily from common, low-cost histology materials and serves as a reliable visual marker in paraffin blocks and sections.
01:18
Design Example: Designing Water Slide
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2025
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed. Bernoulli's principle determines the water's velocity along the slide.
06:14
In vitro Biofilm Formation in an 8-well Chamber Slide
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Cited by 62 •
2011
This article describes the procedure for the formation and visualization of a bacterial biofilm grown within an 8-well chamber...
FAQs
These interactions help retain proteins, antibodies, lysates, and other analytes within localized regions of the porous nitrocellulose surface after droplets make contact. Immobilization keeps each sample associated with its original spot, allowing later probing and signal detection. This retention mechanism is important because it preserves spatial separation while supporting parallel examination of many biological samples.
Porosity provides a surface that can capture material from deposited droplets rather than allowing the analyte to remain entirely in a mobile liquid phase. The resulting localized retention supports defined spot formation and subsequent analysis. In cancer research, this is valuable when small amounts of proteins, antibodies, lysates, or tumor-associated material must be examined across many positions.
Miniaturization places many defined samples or experimental conditions on a single slide while using relatively small amounts of specimen and reagents. This increases the number of comparisons possible within one assay format and helps conserve valuable biological material. The approach therefore supports efficient screening and profiling when researchers need to evaluate numerous cancer-related samples or targets.
A sample containing the selected proteins, antibodies, lysates, or other analytes is deposited as a small droplet onto a nitrocellulose-coated microscope slide. Contact with the porous surface immobilizes the material in a defined spot. The prepared slide can then undergo probing and signal detection, enabling parallel analysis of the deposited samples.
The technique is useful for protein profiling, biomarker screening, antibody validation, and comparative analysis of tumor-associated samples. It can organize many biological specimens or experimental conditions in a compact format, making it suitable for studies that seek patterns across cancer-related materials. Its low-volume design is especially relevant when specimens or reagents are limited.
After immobilized samples are probed and signals are detected, researchers can compare the behavior or measured presence of analytes across defined spots. Such comparisons can support protein profiles, evaluation of candidate biomarkers, and assessment of antibody performance. In tumor-associated studies, the arrangement also enables parallel examination of differences among samples or experimental conditions.