De Novo Lipid Synthesis
De Novo Lipid Synthesis - Related Videos
06:27
Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
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Cited by 5 •
2020
Here, we introduce and describe a nonradioactive and noninvasive method to assess de novo protein synthesis in vivo, utilizing the nematode Caenorhabditis elegans and fluorescence recovery after photobleaching (FRAP). This method can be combined with genetic and/or pharmacological screens to identify novel modulators of protein synthesis.
05:51
A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
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Cited by 11 •
2011
Molecular genetic strategy for finding de novo mutations causing common disorders such as autism and schizophrenia.
07:12
Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
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2024
Presented here is a method for screening lipid structures through stable isotope labeling by using their retention time, mobility, and fragmentation.
07:34
Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
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2023
Here, we present an inexpensive quantitative method utilizing deuterium oxide and gas chromatography mass spectrometry (GCMS) for the analysis of total fatty acid de novo lipogenesis in brown adipose tissue in vivo.
10:41
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms
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Cited by 7 •
2017
This protocol outlines a comparative de novo transcriptome assembly and annotation workflow for novice bioinformaticians. The workflow is available for free entirely through CyVerse and connected by the Data Store. Command line and graphical user interfaces are used, but all code needed is available to copy and paste.
FAQs
Acetyl-CoA is first converted to malonyl-CoA, creating the carbon-building unit used by fatty acid synthase. This conversion prepares the precursor for repeated condensation reactions that extend the growing fatty-acid chain. Examining this step helps researchers connect central carbon metabolism with lipid production and identify where regulation may influence the amount of newly formed fatty acid.
Fatty acid synthase builds the fatty-acid chain through repeated cycles of condensation and reduction. NADPH supplies the reducing power required for these transformations, while malonyl-CoA contributes successive carbon units. The coordinated cycle produces a saturated fatty acid, making fatty acid synthase and NADPH central chemical components of the pathway’s carbon assembly and reduction process.
The initial saturated fatty-acid product can be chemically diversified after its formation. Elongation increases chain length, desaturation introduces additional structural variation, and esterification incorporates fatty acids into larger lipid molecules. Together, these reactions generate a broader lipid set than the central synthesis cycle alone, supporting the formation of membrane components, energy stores, and signaling compounds.
The key distinction is the source of the lipid carbon. De novo production builds fatty acids and other lipids from small carbon precursors, whereas direct uptake obtains lipid material from the surrounding environment. Comparing these routes helps clarify whether cellular lipid composition reflects internal metabolic production, environmental supply, or the balance between both processes.
A pathway-focused analysis follows carbon from acetyl-CoA to malonyl-CoA, through fatty acid synthase, and then into the reactions that modify the resulting saturated fatty acid. Researchers can assess the subsequent roles of elongation, desaturation, and esterification to understand how relatively simple precursors lead to chemically diverse lipid products and cellular functions.
Studying newly produced lipids links reaction chemistry with biological outcomes. The pathway informs investigations of metabolic disease, cancer biology, and nutrition because its regulation affects lipid availability and composition. It also supports research on lipid-based therapeutic strategies, while the resulting molecules provide material for membranes, energy storage, and signaling processes.