Single-pass Perfusion
Single-pass Perfusion - Related Videos
01:25
Single-pass Transmembrane Proteins
0 Views •
2023
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
01:26
Insertion of Single-pass Transmembrane Proteins in the RER
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2023
Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it. Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
01:12
First Pass Effect
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2023
Presystemic elimination, or the first-pass effect, is the metabolism of drugs that reduces their effective concentration at the site of action. Apart from the first-pass effect, the systemic bioavailability of the drug is also reduced by other factors, including incomplete absorption or chemical degradation of drugs. Depending on the route of administration, drugs can be metabolized in the liver, intestine, lungs, and vasculature. Orally administered drugs are first absorbed through the...
07:22
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
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Cited by 5 •
2014
The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
FAQs
The one-way stream creates a defined input-output relationship between the perfusate and the tissue. Materials entering with the fresh stream can be compared with substances appearing in the collected outflow, without those released substances returning to the organ. This separation helps researchers examine exchange across the tissue and improves control over exposure during an experiment.
A pump regulates the perfusate’s movement through the preparation, while controlled pressure helps maintain defined delivery conditions. These variables influence how oxygen, nutrients, drugs, or diagnostic agents contact the tissue and how efficiently released substances leave it. Maintaining controlled conditions supports more consistent assessment of tissue function and makes comparisons between experimental settings more meaningful.
Oxygenated perfusate supplies the gases and nutrients needed while the isolated organ or tissue is being assessed. At the same time, the flowing fluid carries away metabolic products and other substances released by the preparation. This combined delivery and removal function allows investigators to study tissue behavior under defined conditions rather than relying only on an intact circulation.
The key distinction is what happens after the perfusate contacts the tissue. In a single-pass arrangement, the outflow is collected or disposed of instead of being returned to the circuit. That reduces recirculation, limits repeated exposure to previously released substances, and makes it simpler to relate collected outflow to the tissue’s exchanges during the defined perfusion period.
A typical workflow connects the organ, tissue, or vascular preparation to a pump, supplies oxygenated perfusate at controlled flow and pressure, and directs the resulting outflow into a collection pathway. The incoming stream provides the selected nutrients, gases, drugs, or diagnostic agents, while the collected outflow preserves substances released during contact for subsequent assessment.
The perfusate can carry nutrients and gases needed for the preparation, or it can introduce drugs and diagnostic agents for a defined exposure. Because the stream is fresh and its movement is controlled, researchers can examine how the tissue responds to the delivered material while collecting the outflow for analysis of substances exchanged or released.
Clinical researchers can apply the technique to isolated-organ assessment, regional drug delivery, and evaluation of tissue function under controlled conditions. It is particularly useful when investigators need to expose a defined organ or vascular region to selected agents and then examine what leaves the preparation, without the complexity introduced by returning the outflow to the circuit.
The outflow contains substances that the perfused organ, tissue, or vascular system releases during exposure, including metabolic products and other exchanged materials. Examining this collected stream can help researchers evaluate tissue function and characterize responses to delivered nutrients, gases, drugs, or diagnostic agents. Reduced recirculation also simplifies interpretation by limiting the contribution of previously collected material.