Mouse Liver Isolation
Mouse Liver Isolation - Related Videos
03:42
Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
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2025
In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
07:39
Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion
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Cited by 22 •
2012
A technique for isolating portal fibroblasts from rat liver is described. Livers are perfused and digested in situ with collagenase, followed by ex vivo digestion of the liver slurry and size selection of cells. This method provides a pure population of portal fibroblasts without the need for passage in culture.
11:44
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Cited by 3 •
2016
Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.
FAQs
Centrifugation is used after enzymatic digestion, tissue dissociation, and filtration to separate cellular fractions. In this context, it helps obtain hepatocytes apart from nonparenchymal populations, including Kupffer cells and lymphocytes. Researchers can then examine parenchymal and immune-associated compartments independently using flow cytometry, cell culture, molecular assays, or functional studies.
Enzymatic digestion and tissue dissociation convert prepared liver tissue into a cell suspension suitable for downstream separation and analysis. This step is particularly important when researchers need individual hepatocytes or nonparenchymal cells rather than intact tissue. Subsequent filtration and centrifugation help produce preparations compatible with cellular assays and population-specific investigations.
Preserving liver structure maintains the tissue format needed for microscopy and other analyses that examine the organ as a whole. This perspective complements studies of isolated cells by connecting cellular findings with liver physiology, immune-cell recruitment, inflammatory signaling, and disease progression. It therefore supports investigation of host responses without relying only on dissociated-cell measurements.
A typical workflow begins with sterile dissection and vascular perfusion, followed by enzymatic digestion and tissue dissociation. Filtration helps prepare the resulting material, while centrifugation supports separation of hepatocytes from nonparenchymal cells. The sequence is important because it moves from tissue recovery to cellular preparation, enabling the resulting samples to be directed toward distinct analytical methods.
Intact tissue is most useful when the analysis depends on preserved structure, including microscopy-based investigation. Dissociated cells are better suited to applications requiring separated populations, such as flow cytometry, cell culture, molecular assays, or functional studies. Comparing these preparation types allows researchers to evaluate both tissue-level features and the properties of specific hepatocyte or immune-cell compartments.
Isolated liver tissue and cells provide complementary ways to study pathogen burden, immune-cell recruitment, inflammatory signaling, and host–pathogen interactions. In infection studies, these preparations can help relate local liver findings to systemic immune responses and disease progression. Their compatibility with microscopy, flow cytometry, culture, and molecular assays also allows investigators to examine these processes from multiple perspectives.