Photometer
Photometer - Related Videos
06:27
Measurement of Aerosols Optical Thickness of the Atmosphere using the GLOBE Handheld Sun Photometer
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Cited by 4 •
2019
The goal of the methods presented here is to measure aerosol optical thickness of the atmosphere. The sun photometer is pointed at the sun and the largest voltage reading obtained on an in-built digital voltmeter is recorded. Atmospheric measurements such as barometric pressure and relative humidity are also performed.
05:14
Minimum Erythematous Dose Assay: A Method for Measuring UV Sensitivity in Mouse Models
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2023
This video describes a method to measure UV sensitivity in a humanized mouse model—a mouse model that mimics human skin. We determine the minimal erythematous dose (MED) of UV radiation that can cause erythema or edema on the skin. The method can help to find out the efficacy of drugs used to reduce UV sensitivity.
05:16
Mass Photometry to Study Antigen-Antibody Interactions on a Single-Molecule Level
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2025
In this video, we demonstrate the use of mass photometry to study antigen-antibody interactions in a flow chamber. The focused light scatters differently based on the mass of the molecules. A detector collects the scattered light and generates the interference pattern as spots of varied intensities, which correlate with the molecular masses.
02:57
Bacterial Cell Staining and Preparation for Immunological Studies
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2025
This video demonstrates the staining and preparation of bacterial cells for immunological studies using red fluorescent dye. The stained bacteria are diluted and resuspended in a growth medium to achieve the desired bacterial concentration for host cell infection, representing the multiplicity of infection.
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Cited by 10 •
2017
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
FAQs
Under defined conditions, absorbance changes in relation to analyte concentration according to the Beer-Lambert relationship. This allows a measured optical signal to serve as a quantitative indicator rather than merely a visual color change. The relationship is most useful when researchers maintain consistent conditions so readings from comparable samples can be interpreted together.
These signal types represent different ways that a sample interacts with light. Measuring transmitted light assesses what passes through the sample, while absorbance reflects light removed from that path; emitted light captures radiation produced by the sample. Selecting the relevant signal helps match the measurement to the assay and the property being quantified.
Consistent conditions make optical readings more suitable for comparison across samples and experiments. When conditions vary, changes in the measured signal may be harder to attribute to differences in the biological substance itself. Standardized measurements therefore support quantitative experiments, including comparisons of assay results, protein levels, or infection-related biological changes.
In a colorimetric ELISA, the photometer measures the assay-generated color signal and converts differences in that signal into quantitative readings. Researchers can use those readings to estimate antibody or antigen levels rather than relying only on visual inspection. This supports comparisons among samples and helps standardize immunological measurements.
Photometer measurements in protein assays provide numerical signals that help quantify or compare protein-associated material in samples. Because the instrument detects changes in light interaction, researchers can use the resulting values to evaluate biological substances under consistent conditions. This makes the method useful for standardizing protein measurements across experimental samples.
In infection research, photometer readings can be used to monitor microbial growth or metabolic activity. Changes in the measured signal provide quantitative information about pathogen-associated behavior over experimental comparisons. This complements immunological measurements, such as estimating antibody or antigen levels, by linking optical analysis to biological changes associated with microbes.