Surgical Model Preparation
Surgical Model Preparation - Related Videos
01:32
Pig Surgical Preparation and Postoperative Care
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Surgical Preparation and Physiological Monitoring This video provides an introductory overview of surgical preparation, intraoperative monitoring, and postoperative care in swine. It is intended to familiarize learners with key...
04:36
Vacuum-Sealed Hot Water Bath Immersion for the Preparation of Anatomical and Surgical Cadaveric Bone Models
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Cited by 1 •
2022
The present protocol describes the maceration and cleaning of cadaveric bone with a vacuum-sealed, hot water bath immersion technique. This is a low-cost, safe, and effective method to produce anatomical specimens for surgical planning and medical education as an alternative to three-dimensional (3D) printed models.
05:19
Surgical Models of Gastroesophageal Reflux with Mice
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Cited by 8 •
2015
This article demonstrates surgical procedures of gastroesophageal reflux with mice. These models are useful tools for research on mechanisms and treatment of gastroesophageal reflux disease and potentially Barrett’s esophagus and esophageal adenocarcinoma.
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
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2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
01:36
Rabbit Preoperative Preparation, Surgical Procedures, and Postoperative Care
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Preoperative Preparation The initial assessment should include observation of alertness, posture, gait, and grooming behavior, as changes in these parameters may indicate underlying health issues. Body weight should be recorded...
FAQs
Model selection determines which physiological or disease process the experiment can examine and how immune or infectious outcomes should be interpreted. A localized infection model can support analysis of site-specific inflammation and immune-cell recruitment, whereas a systemic-response model can address pathogen dissemination and broader host reactions. Matching the model to the research question improves comparability.
Anesthesia and aseptic handling help separate intended experimental effects from avoidable procedural variation. Anesthesia is part of the controlled operative conditions, while aseptic practice limits unintended microbial exposure during tissue access. Together, they make tissue injury and exposure conditions more consistent, helping investigators attribute inflammation, immune-cell recruitment, or treatment responses to the study design.
Postoperative monitoring is important because the host response continues after the operative intervention. Observing animals after surgery helps maintain standardized conditions and supports assessment of responses associated with tissue injury, microbial exposure, or treatment. This step also connects experimental control with animal welfare, which is essential when interpreting changes in inflammation or systemic immunity.
Planning should establish the model, the intended tissue or infection context, the anesthesia approach, aseptic handling, surgical access, and postoperative monitoring. These elements should be aligned before the operation so that tissue injury, microbial exposure, and host responses remain controlled. A consistent plan also supports reliable comparisons between experimental groups and across studies.
The resulting model can be used to examine pathogen dissemination, inflammation, immune-cell recruitment, and treatment effects. The most informative outcome depends on whether the design emphasizes a localized infection site or a systemic immune response. Linking these measurements to the planned operative conditions helps researchers interpret whether observed changes reflect infection, host immunity, or intervention.
Standardization reduces experimental variability by keeping operative conditions comparable across animals and experiments. That consistency strengthens interpretation of differences in pathogen spread, inflammatory responses, immune-cell recruitment, or treatment effects. It also enables comparisons between localized and systemic study designs while supporting animal welfare through planned anesthesia, aseptic handling, and postoperative monitoring.