Maternal Obesity Model
Maternal Obesity Model - Related Videos
11:44
A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
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Cited by 11 •
2017
Presented here is a protocol for sampling of human placental villous tissue followed by isolation of cytotrophoblasts for primary cell culture. Treatment of trophoblasts with TNFα recapitulates inflammation in the obese intrauterine environment and facilitates the discovery of molecular targets regulated by inflammation in placentas with maternal obesity.
09:17
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
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2025
Here, we describe a protocol for generating maternal mutant that couples a stable zpc:cas9 knock-in line with Tol2-mediated delivery of sgRNA expression cassettes.
13:09
Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography
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Cited by 26 •
2012
Fat content analysis is routinely conducted in studies utilizing murine obesity models. Emerging methods in small animal CT imaging and analysis are providing for longitudinal detail rich fat content analysis. Here we detail step by step procedures for performing small animal CT imaging, analysis, and visualization.
09:49
Assessment of Perigenital Sensitivity and Prostatic Mast Cell Activation in a Mouse Model of Neonatal Maternal Separation
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Cited by 25 •
2015
We are measuring perigenital mechanical sensitivity and mast cell activation in the prostate of male C57BL/6 mice that underwent an early life stress paradigm – neonatal maternal separation, in order to induce a preclinical model of chronic prostatitis/chronic pelvic pain syndrome.
07:51
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Cited by 44 •
2015
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
FAQs
It allows researchers to connect maternal adiposity during pregnancy with changes in the intrauterine environment and later offspring health. By examining fetal development alongside postnatal outcomes, investigators can study how prenatal exposure may increase susceptibility to metabolic disease. This approach links events during gestation with health patterns that emerge after birth.
Key pathways include insulin resistance, inflammation, placental function, and nutrient transfer. Studying these processes helps clarify how excess maternal adiposity may alter the conditions supporting fetal growth. Measuring them together can reveal whether pregnancy complications or offspring metabolic risk are associated with disrupted maternal metabolism, placental activity, or the movement of nutrients to the fetus.
The placenta is examined because it contributes to the exchange of nutrients between mother and fetus. Changes in placental function or nutrient transfer may help explain how maternal metabolic changes influence fetal development and growth. Including placental assessments therefore connects maternal physiology with fetal outcomes rather than evaluating maternal obesity only through postnatal measurements.
These approaches reproduce maternal obesity through different experimental routes, either by altering diet, using genetic factors, or applying metabolic approaches. Comparing them can help determine whether observed pregnancy and offspring outcomes are consistent across model types or depend on how excess adiposity was produced. The choice of approach therefore affects interpretation of the experimental findings.
A study may assess maternal insulin resistance and inflammation, then examine placental function, nutrient transfer, fetal development, and growth. Follow-up after birth can evaluate offspring health and susceptibility to metabolic disease. Collecting measurements across pregnancy and after delivery helps researchers relate maternal conditions to both immediate developmental outcomes and longer-term effects.
Researchers use this model to investigate pregnancy complications, fetal growth, and offspring metabolic risk, while also exploring how the intrauterine environment shapes later health. It can support evaluation of preventive strategies and interventions intended to improve maternal and child health. The model is especially useful when studies need to connect maternal physiology with outcomes spanning pregnancy and postnatal life.