Myograph Chamber
Myograph Chamber - Related Videos
07:25
Assessing Murine Resistance Artery Function Using Pressure Myography
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Cited by 18 •
2013
In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
09:47
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
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Cited by 1 •
2022
The present protocol describes the concepts and technical application of the tensometric myograph technique using a multi-chamber myograph system in the experimental ex vivo assessment of mouse aortic endothelial function.
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
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Cited by 8 •
2012
Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
07:51
Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
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Cited by 37 •
2011
An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.
10:48
Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
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Cited by 24 •
2015
Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.
FAQs
An isometric setup records changes in tension while the tissue remains mounted between a fixed support and a movable support. The force transducer converts generated force into a measurable signal. This arrangement lets investigators compare contractile responses while maintaining controlled experimental conditions and using force change as the primary experimental endpoint.
Resting tension, temperature, and physiological-solution composition can alter the response observed after stimulation. Researchers therefore adjust these conditions deliberately rather than treating them as incidental details. Holding them under control helps distinguish changes caused by an applied drug or electrical stimulus from changes associated with the experimental environment.
Contraction and relaxation provide complementary information about vascular reactivity. A contraction response shows how the tissue develops force, whereas relaxation shows how that force changes after an intervention. Examining both patterns helps characterize smooth muscle function and pharmacological responses, including whether a tested condition produces different effects on vessel tissue.
A typical workflow begins by mounting a vessel ring or other tissue segment between fixed and movable supports. The preparation is placed in oxygenated physiological solution, then resting tension, temperature, and solution composition are adjusted. Researchers apply a drug or electrical stimulation and use the transducer recording to evaluate the resulting tension change.
The core arrangement combines a tissue support system, an oxygenated physiological solution, and a force transducer. Fixed and movable supports hold the preparation, while the transducer records changes in isometric tension. Together, these components allow researchers to expose the tissue to controlled drugs or electrical stimulation and observe force responses.
In medicine-related research, myograph chamber studies can characterize vascular reactivity and smooth muscle function, compare pharmacological responses, and evaluate potential therapeutic agents. The resulting force measurements are relevant to investigations of hypertension and cardiovascular disease because they show how isolated blood-vessel tissue responds under controlled conditions.