High-throughput Imaging
High-throughput Imaging - Related Videos
10:18
High-Throughput, Multi-Image Cryohistology of Mineralized Tissues
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Cited by 117 •
2016
In this manuscript, we present a high-throughput, semi-automated cryohistology platform to produce aligned composite images of multiple response measures from several rounds of fluorescent imaging on frozen sections of mineralized tissues.
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
06:19
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
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Cited by 16 •
2023
This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.
08:19
Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
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Cited by 24 •
2009
Lensfree on-chip imaging and characterization of cells is illustrated. This on-chip cell imaging approach provides a compact and cost-effective tool for medical diagnostics and high-throughput cell biology applications, making it especially suitable for resource poor settings.
FAQs
Standardization links image acquisition to computational analysis so that measurements remain comparable across many samples. Researchers can define consistent imaging conditions and apply a data pipeline to quantify selected cellular or tissue features rather than relying on individually chosen images. This supports systematic comparison across treatments, developmental stages, or disease models and strengthens reproducibility in large neuroscience experiments.
Robotic sample handling and rapid microscopy address different parts of the scale problem. Automation moves or presents samples consistently, while rapid imaging collects measurements efficiently; together, they reduce the need for manual selection of fields or specimens. In neuroscience, this consistency helps compare neuronal morphology, protein localization, synaptic markers, or activity-related fluorescence under controlled experimental conditions.
Computational image analysis converts captured images into comparable measurements by focusing on defined cellular or tissue features. In this setting, the pipeline can quantify neuronal morphology, protein localization, synaptic markers, or activity-related fluorescence across many samples. The resulting measurements provide a consistent basis for comparing experimental groups instead of depending primarily on visual inspection or manual selection.
A practical experiment starts by organizing biological samples and experimental conditions for robotic handling, followed by rapid microscopy under controlled conditions. Image data then pass through a computational analysis pipeline that extracts the chosen signals or structural features. Researchers can use this workflow across treatments, developmental stages, or disease models, allowing the same measurement strategy to be applied systematically.
High-throughput imaging is especially useful when a neuroscience study must compare many treatments or biological states. Researchers can screen treatments, examine developmental changes, or evaluate disease models while measuring neuronal morphology, synaptic markers, protein localization, or activity-related fluorescence. These readouts support phenotypic screening and can reveal patterns that guide later investigation of cellular or circuit-level mechanisms.
In neuroscience, the approach connects cell-level measurements with broader experimental questions. Imaging data can characterize neuronal morphology and molecular localization, assess synaptic features, or measure activity-related fluorescence across standardized conditions. Such comparisons may help identify candidate mechanisms or therapeutics, while also supporting circuit and cell biology studies. Its value comes from relating many quantified observations to defined experimental groups.