High-order Shimming

High-order shimming is a precision technique for improving the spatial uniformity of a magnetic field by correcting complex inhomogeneities that lower-order adjustments cannot remove. It uses additional shim-field components, often represented by higher-order spherical harmonics, whose strengths are optimized from measured field maps to compensate position-dependent deviations. In magnetic resonance physics, this process improves field homogeneity across an imaging or spectroscopy volume, producing narrower spectral lines, reduced artifacts, and more reliable quantitative measurements. High-order shimming is especially valuable for challenging sample geometries, large fields of view, and applications requiring high spatial or chemical-shift resolution.

High-order Shimming - Related Videos

Research

JoVE Journal - Medicine
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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

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Cited by 3 •

2020

We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.

Electrochemiluminescence Assays for Human Islet Autoantibodies

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Cited by 8 •

2018

Here, we presented the methods to detect human islet autoantibodies using electrochemiluminescence (ECL) assays. The protocol, used to predict type 1 diabetes, can be expanded upon to detect autoantibodies for other autoimmune diseases.

Research

JoVE EoE - Spectroscopy Techniques

Nuclear Magnetic Resonance Spectroscopy to Identify Multiple Phosphorylations in Proteins

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2025

This video demonstrates the use of nuclear magnetic resonance spectroscopy (NMR) techniques to identify multiple phosphorylations in a protein. The phosphorylation of a protein at specific amino acid causes the deshielding of the neighboring amide hydrogen, which generates the spectral difference.

Cardiac Magnetic Resonance Imaging at 7 Tesla

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Cited by 9 •

2019

The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Cited by 17 •

2017

We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.

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