Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of <em>Arabidopsis thaliana</em> Suspension Cell Cultures

Witold G. Szymanski; Sylwia Kierszniowska; Waltraud X. Schulze

doi:10.3791/50535

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Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of <em>Arabidopsis thaliana</em> Suspension Cell Cultures

JoVE Journal
Biologia

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JoVE Journal Biologia

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

14,019 Views

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11:44 min

•

September 28, 2013

DOI:

10.3791/50535-v

DOI:

10.3791/50535-v

•

11:44 min

September 28, 2013

•

6 Views

Witold G. Szymanski¹, Sylwia Kierszniowska¹, Waltraud X. Schulze^1,2

¹Signaling Proteomics,Max Plank Institute of Molecular Plant Physiology, ²Department of Plant Systems Biology,University of Hohenheim

Podsumowanie

Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully ¹⁵N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.