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March 31, 2014
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We describe the use of styryl FM dyes to image synaptic vesicle recycling in functional nerve terminals. This protocol can be applied not only to evoked, but also spontaneous and miniature synaptic activities. The protocol expands the variety of synaptic events that can be effectively evaluated.
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Iwabuchi, S., Kakazu, Y., Koh, J., Goodman, K. M., Harata, N. C. Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities. J. Vis. Exp. (85), e50557, doi:10.3791/50557 (2014).
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