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July 24, 2014
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Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.
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Chen, K. G., Hamilton, R. S., Robey, P. G., Mallon, B. S. Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis. J. Vis. Exp. (89), e51519, doi:10.3791/51519 (2014).
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