Dziennik
/
Immunologia i Infekcje
/
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
<em>In Situ</em> Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
JoVE Journal
Immunologia i Infekcje
Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE.  Zaloguj się lub rozpocznij bezpłatny okres próbny.
JoVE Journal Immunologia i Infekcje
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

English

Automatycznie wygenerowane
Full Text

11,618 Views

•

13:10 min

•

August 01, 2014

DOI:

10.3791/51679-v

DOI:
10.3791/51679-v

•

13:10 min
August 01, 2014

•

5 Views
, , , , , ,
1School of Veterinary Medicine and Biomedical Sciences,University of Nebraska, Lincoln, 2Center for Biotechnology,University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences,University of Nebraska, Lincoln

Podsumowanie

Automatically generated

The protocol to detect self-reactive CD4 T cells in brain and heart by direct staining with major histocompatibility complex class II dextramers has been described in this report. For comprehensive analysis, a reliable method to enumerate the frequencies of antigen-specific CD4+ T cells in situ is also devised.

Przeglądaj więcej filmów

MHC Class II DextramersAutoreactive CD4 T CellsExperimental Autoimmune Encephalomyelitis (EAE)Experimental Autoimmune Myocarditis (EAM)Laser Scanning Confocal Microscope (LSCM)Myelin Proteolipid Protein (PLP) 139-151Cardiac Myosin Heavy Chain-α (Myhc) 334-352Theiler’s Murine Encephalomyelitis Virus (TMEV) 70-86Bovine Ribonuclease (RNase) 43-56In Situ DetectionQuantitative Analysis

Powiązane Filmy

Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients

09:58

Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients

Powiązane Filmy

40706 Views

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

15:42

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Powiązane Filmy

21663 Views

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

08:11

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Powiązane Filmy

8651 Views

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

10:19

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Powiązane Filmy

16210 Views

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

11:28

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Powiązane Filmy

10599 Views

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

09:20

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Powiązane Filmy

18133 Views

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Powiązane Filmy

13394 Views

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

08:37

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Powiązane Filmy

12196 Views

Isolation and Identification of Extravascular Immune Cells of the Heart

08:24

Isolation and Identification of Extravascular Immune Cells of the Heart

Powiązane Filmy

12579 Views

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

09:15

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Powiązane Filmy

851 Views

Read Article

Cytuj ten artykuł

Massilamany, C., Gangaplara, A., Jia, T., Elowsky, C., Li, Q., Zhou, Y., Reddy, J. In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers. J. Vis. Exp. (90), e51679, doi:10.3791/51679 (2014).

Download .ris file

Copy

Udostępnij film

.

Copy