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Isolation of Myofibroblasts from Mouse and Human Esophagus
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Myofibroblasts from Mouse and Human Esophagus

Isolation of Myofibroblasts from Mouse and Human Esophagus

English

Automatycznie wygenerowane

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11:18 min

January 18, 2015

DOI:

11:18 min
January 18, 2015

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Podsumowanie

Automatically generated

We present a protocol to generate primary cultures of murine and human esophageal stromal cells with a myofibroblast phenotype. Cultured cells have spindle shaped morphology, express α-SMA and vimentin, and lack epithelial, hematopoietic and endothelial cell surface markers. Characterized stromal cells can be used in functional studies of epithelial-stromal interactions.

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