13,388 Views
•
11:22 min
•
February 17, 2015
DOI:
Podsumowanie
Automatically generated
A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.
Powiązane Filmy
08:59
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Powiązane Filmy
8130 Views
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Powiązane Filmy
22501 Views
07:13
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
Powiązane Filmy
12546 Views
09:53
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
Powiązane Filmy
6614 Views
08:46
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Powiązane Filmy
11648 Views
07:50
An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues
Powiązane Filmy
6786 Views
09:24
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
Powiązane Filmy
9709 Views
08:06
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
Powiązane Filmy
5977 Views
11:02
An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
Powiązane Filmy
2500 Views
05:28
Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
Powiązane Filmy
2841 Views
Read Article
Cytuj ten artykuł
Su, C. T. E., Yoon, S., Marcy, G., Chin, E. W. M., Augustine, G. J., Goh, E. L. K. An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System. J. Vis. Exp. (96), e52408, doi:10.3791/52408 (2015).
Copy