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April 14, 2015
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Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.
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Moorthy, B. S., Iyer, L. K., Topp, E. M. Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders. J. Vis. Exp. (98), e52503, doi:10.3791/52503 (2015).
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