Technique to Target Microinjection to the Developing <em>Xenopus</em> Kidney

Bridget D. DeLay; Vanja Krneta-Stankic; Rachel K. Miller

doi:10.3791/53799

Dziennik

/

Biologia Rozwoju

/

Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing <em>Xenopus</em> Kidney

JoVE Journal
Biologia Rozwoju

Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE. Zaloguj się lub rozpocznij bezpłatny okres próbny.

JoVE Journal Biologia Rozwoju

Technique to Target Microinjection to the Developing Xenopus Kidney

Technique to Target Microinjection to the Developing Xenopus Kidney

11,184 Views

•

11:29 min

•

May 03, 2016

DOI:

10.3791/53799-v

DOI:

10.3791/53799-v

•

11:29 min

May 03, 2016

•

1 Views

Bridget D. DeLay¹, Vanja Krneta-Stankic^1,2, Rachel K. Miller^1,2,3,4

¹Department of Pediatrics, Pediatric Research Center,University of Texas McGovern Medical School, ²Program in Genes & Development,University of Texas Graduate School of Biomedical Sciences, ³Program in Cell & Regulatory Biology,University of Texas Graduate School of Biomedical Sciences, ⁴Department of Genetics,University of Texas MD Anderson Cancer Center

Transkrypcja

Podsumowanie

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

Przeglądaj więcej filmów

Microinjection Xenopus Kidney Biologia Rozwoju Testis Isolation Egg Collection Fertilization De-jelling Temperature Control Membrane-bound Red Fluorescent Protein MRNA

Powiązane Filmy

Read Article