Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

Yaneri A. Ayala; David P&#233;rez-Gonz&#225;lez; Daniel Duque; Alan R. Palmer; Manuel S. Malmierca

doi:10.3791/53914

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Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

JoVE Journal
Neurologia

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JoVE Journal Neurologia

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

9,961 Views

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11:19 min

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May 21, 2016

DOI:

10.3791/53914-v

DOI:

10.3791/53914-v

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11:19 min

May 21, 2016

•

3 Views

Yaneri A. Ayala¹, David Pérez-González¹, Daniel Duque^1,2, Alan R. Palmer³, Manuel S. Malmierca^1,4

¹Auditory Neuroscience Laboratory, Institute of Neuroscience of Castilla y León,University of Salamanca, ²Neural Systems Laboratory, Institute for Systems Research,University of Maryland, ³Medical Research Council Institute of Hearing Research, ⁴Department of Cell Biology and Pathology, Faculty of Medicine,University of Salamanca

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Podsumowanie

We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of interest.