We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.
Kim, H. J., Lee, J., Choi, J., Bahinski, A., Ingber, D. E. Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device. J. Vis. Exp. (114), e54344, doi:10.3791/54344 (2016).