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November 30, 2016
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We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.
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Fourie, N. H., Peace, R. M., Abey, S. K., Sherwin, L. B., Wiley, J. W., Henderson, W. A. Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform. J. Vis. Exp. (117), e54693, doi:10.3791/54693 (2016).
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