The overall goal of this protocol is to accurately align magnetic resonance imaging (MRI) image volumes with histology sections via the creation of customized 3D-printed brain holders and slicer boxes.
Luciano, N. J., Sati, P., Nair, G., Guy, J. R., Ha, S., Absinta, M., Chiang, W., Leibovitch, E. C., Jacobson, S., Silva, A. C., Reich, D. S. Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning. J. Vis. Exp. (118), e54780, doi:10.3791/54780 (2016).