16,358 Views
•
10:50 min
•
February 25, 2017
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.
Powiązane Filmy
10:23
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Powiązane Filmy
10895 Views
11:37
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Powiązane Filmy
17538 Views
09:25
Isolation and Characterization of Single Cells from Zebrafish Embryos
Powiązane Filmy
14463 Views
09:34
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Powiązane Filmy
6609 Views
07:30
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
Powiązane Filmy
11912 Views
06:26
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Powiązane Filmy
3096 Views
09:26
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Powiązane Filmy
15352 Views
09:13
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
Powiązane Filmy
8001 Views
08:23
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Powiązane Filmy
17325 Views
09:03
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Powiązane Filmy
11465 Views
Read Article
Cytuj ten artykuł
Porter, J. R., Telford, W. G., Batchelor, E. Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards. J. Vis. Exp. (120), e55219, doi:10.3791/55219 (2017).
Copy