9,491 Views
•
08:09 min
•
March 24, 2017
DOI:
Podsumowanie
Automatically generated
Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.
Powiązane Filmy
13:49
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Powiązane Filmy
11408 Views
08:07
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Powiązane Filmy
10078 Views
13:58
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Powiązane Filmy
17948 Views
08:04
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
Powiązane Filmy
6958 Views
13:14
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Powiązane Filmy
10365 Views
N/A
Tetrameric Fluorescent Antigen Arrays for Single-Step Identification of Antigen-Specific B Cells
Powiązane Filmy
1863 Views
00:09
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Powiązane Filmy
10649 Views
10:37
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Powiązane Filmy
2907 Views
07:59
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Powiązane Filmy
14928 Views
11:48
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
Powiązane Filmy
14179 Views
Read Article
Cytuj ten artykuł
Chen, C., Chang, C. Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope. J. Vis. Exp. (121), e55417, doi:10.3791/55417 (2017).
Copy