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May 16, 2017
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This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.
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Moriya, T., Saur, M., Stabrin, M., Merino, F., Voicu, H., Huang, Z., Penczek, P. A., Raunser, S., Gatsogiannis, C. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J. Vis. Exp. (123), e55448, doi:10.3791/55448 (2017).
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