Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Xianming Yang; Pengjun Xu; Robert I. Graham; He Yuan; Kongming Wu

doi:10.3791/55534

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Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

JoVE Journal
Immunologia i Infekcje

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JoVE Journal Immunologia i Infekcje

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

8,477 Views

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11:12 min

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April 12, 2017

DOI:

10.3791/55534-v

DOI:

10.3791/55534-v

•

11:12 min

April 12, 2017

•

8449 Views

Xianming Yang¹, Pengjun Xu², Robert I. Graham³, He Yuan¹, Kongming Wu¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences ²Tobacco Research Institute,Chinese Academy of Agricultural Sciences ³Crop and Environment Sciences,Harper Adams University

Podsumowanie

Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.