9,191 Views
•
10:23 min
•
April 20, 2017
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.
Powiązane Filmy
15:02
Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
Powiązane Filmy
10710 Views
07:20
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
Powiązane Filmy
2484 Views
07:47
Microscopy of Fission Yeast Sexual Lifecycle
Powiązane Filmy
14432 Views
10:25
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
Powiązane Filmy
11028 Views
10:52
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Powiązane Filmy
9693 Views
11:19
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Powiązane Filmy
7320 Views
12:00
4D Microscopy of Yeast
Powiązane Filmy
8465 Views
12:04
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Powiązane Filmy
9440 Views
08:31
Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
Powiązane Filmy
6153 Views
06:50
Super-Resolution Live Cell Imaging of Subcellular Structures
Powiązane Filmy
4673 Views
Read Article
Cytuj ten artykuł
Fees, C. P., Estrem, C., Moore, J. K. High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast. J. Vis. Exp. (122), e55610, doi:10.3791/55610 (2017).
Copy