Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Frank J. Jenkins; Charles M. Kerr; Elise Fouquerel; Dana H. Bovbjerg; Patricia L. Opresko

doi:10.3791/56001

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Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

JoVE Journal
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Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

12,790 Views

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11:29 min

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July 10, 2017

DOI:

10.3791/56001-v

DOI:

10.3791/56001-v

•

11:29 min

July 10, 2017

•

5 Views

Frank J. Jenkins^1,2, Charles M. Kerr², Elise Fouquerel^2,3, Dana H. Bovbjerg^2,4, Patricia L. Opresko^2,3

¹Departments of Pathology and Infectious Diseases and Microbiology,University of Pittsburgh, ²University of Pittsburgh Cancer Institute, ³Department of Environmental and Occupational Health,University of Pittsburgh, ⁴Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences,University of Pittsburgh

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Podsumowanie

In this article, a detailed protocol for quantifying telomere length using a modified terminal restriction fragment analysis is discussed that provides fast and efficient direct measurement of telomere length. This technique can be applied to a variety of cell sources of DNA for quantifying telomere length.