Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-<em>O</em>-thiophenylmethyl Modification and Characterization via Circular Dichroism

Andrew J. Francis; Marino J. E. Resendiz

doi:10.3791/56189

/

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-<em>O</em>-thiophenylmethyl Modification and Characterization via Circular Dichroism

JoVE Journal
Chemia

Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE. Zaloguj się lub rozpocznij bezpłatny okres próbny.

JoVE Journal Chemia

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

18,866 Views

•

11:37 min

•

July 28, 2017

DOI:

10.3791/56189-v

DOI:

10.3791/56189-v

•

11:37 min

July 28, 2017

•

6 Views

Andrew J. Francis¹, Marino J. E. Resendiz¹

¹Department of Chemistry,University of Colorado Denver

Transkrypcja

Podsumowanie

This article provides a detailed procedure on the solid-phase synthesis, purification, and characterization of dodecamers of RNA modified at the C2'-O-position. UV-vis and circular dichroism photometric analyses are used to quantify and characterize structural aspects, i.e., single-strands or double-strands.